Microchannel-patterned and heparin micro-contact-printed biodegradable composite membranes for tissue-engineering applications
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/20665 |
Resumo: | Microchannel-patterned starch–poly(capro-lactone)/hydydroxyapatite (SPCL–HA) and starch– poly(lactic acid) (SPLA) composite membranes were produced for use as a laminated tissueengineering scaffold that incorporates both physical and biochemical patterns. For this purpose, SPCL (30% starch) blended with inorganic hydroxyl apatite (50%) and SPLA (50% starch) membranes were made with compressive moulding. Consequently, the microchannel structures (width 102 μm, 174 μm intervals) were developed on the composite membranes by means of micro-patterned metal mould(s) and hydraulic pressing. An elastomer poly(dimetylsiloxane) stamp was used to transfer heparin as a biochemical cue over the microchannel surfaces by micro-contact printing (μCP). Toluidine blue staining of developed capillaries and heparin μCP-coated membranes showed that heparin was transferred predominantly over the microchannel surfaces. Fibroblast cell culture over the microchannel-formed and heparin μCP-modified SPCL–HA and SPLA membranes showed distinct growth patterns. In contrast to the uniform cell layer formed on unmodified microchannels, the cells were bridging across the grooves of heparin-printed microchannels. At extended culture periods, the heparin-printed microchannels were covered with a layer of fibroblast cells without cellular ingrowths inside. This study indicated that the topographical pattern could induce an organization of fibroblasts only with the biochemical cue and the cells’ functions can be controlled spatially over the microchannels by using both cues. |
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Microchannel-patterned and heparin micro-contact-printed biodegradable composite membranes for tissue-engineering applicationsMicrochannelMicropatterningComposite biomaterialsMembranesFibroblastTissue engineeringScience & TechnologyMicrochannel-patterned starch–poly(capro-lactone)/hydydroxyapatite (SPCL–HA) and starch– poly(lactic acid) (SPLA) composite membranes were produced for use as a laminated tissueengineering scaffold that incorporates both physical and biochemical patterns. For this purpose, SPCL (30% starch) blended with inorganic hydroxyl apatite (50%) and SPLA (50% starch) membranes were made with compressive moulding. Consequently, the microchannel structures (width 102 μm, 174 μm intervals) were developed on the composite membranes by means of micro-patterned metal mould(s) and hydraulic pressing. An elastomer poly(dimetylsiloxane) stamp was used to transfer heparin as a biochemical cue over the microchannel surfaces by micro-contact printing (μCP). Toluidine blue staining of developed capillaries and heparin μCP-coated membranes showed that heparin was transferred predominantly over the microchannel surfaces. Fibroblast cell culture over the microchannel-formed and heparin μCP-modified SPCL–HA and SPLA membranes showed distinct growth patterns. In contrast to the uniform cell layer formed on unmodified microchannels, the cells were bridging across the grooves of heparin-printed microchannels. At extended culture periods, the heparin-printed microchannels were covered with a layer of fibroblast cells without cellular ingrowths inside. This study indicated that the topographical pattern could induce an organization of fibroblasts only with the biochemical cue and the cells’ functions can be controlled spatially over the microchannels by using both cues.E. T. Baran thanks the Portuguese Foundation for Science and Technology (FCT) for providing a post-doctoral scholarship (No. SFRH/BPD/30768/2006). This work was partially supported by the FCT through funds from the POCTI and/or FEDER programmes and also by the European Union-funded STREP Project HIPPOCRATES (Grant No. NMP3-CT-2003-505758).WileyUniversidade do MinhoBaran, Erkan TürkerTuzlakoglu, K.Salgado, A. J.Reis, R. L.2011-062011-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/20665eng1932-700510.1002/term.36821604378http://onlinelibrary.wiley.com/doi/10.1002/term.368/pdfinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:14:53Zoai:repositorium.sdum.uminho.pt:1822/20665Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:07:14.431844Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Microchannel-patterned and heparin micro-contact-printed biodegradable composite membranes for tissue-engineering applications |
title |
Microchannel-patterned and heparin micro-contact-printed biodegradable composite membranes for tissue-engineering applications |
spellingShingle |
Microchannel-patterned and heparin micro-contact-printed biodegradable composite membranes for tissue-engineering applications Baran, Erkan Türker Microchannel Micropatterning Composite biomaterials Membranes Fibroblast Tissue engineering Science & Technology |
title_short |
Microchannel-patterned and heparin micro-contact-printed biodegradable composite membranes for tissue-engineering applications |
title_full |
Microchannel-patterned and heparin micro-contact-printed biodegradable composite membranes for tissue-engineering applications |
title_fullStr |
Microchannel-patterned and heparin micro-contact-printed biodegradable composite membranes for tissue-engineering applications |
title_full_unstemmed |
Microchannel-patterned and heparin micro-contact-printed biodegradable composite membranes for tissue-engineering applications |
title_sort |
Microchannel-patterned and heparin micro-contact-printed biodegradable composite membranes for tissue-engineering applications |
author |
Baran, Erkan Türker |
author_facet |
Baran, Erkan Türker Tuzlakoglu, K. Salgado, A. J. Reis, R. L. |
author_role |
author |
author2 |
Tuzlakoglu, K. Salgado, A. J. Reis, R. L. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Baran, Erkan Türker Tuzlakoglu, K. Salgado, A. J. Reis, R. L. |
dc.subject.por.fl_str_mv |
Microchannel Micropatterning Composite biomaterials Membranes Fibroblast Tissue engineering Science & Technology |
topic |
Microchannel Micropatterning Composite biomaterials Membranes Fibroblast Tissue engineering Science & Technology |
description |
Microchannel-patterned starch–poly(capro-lactone)/hydydroxyapatite (SPCL–HA) and starch– poly(lactic acid) (SPLA) composite membranes were produced for use as a laminated tissueengineering scaffold that incorporates both physical and biochemical patterns. For this purpose, SPCL (30% starch) blended with inorganic hydroxyl apatite (50%) and SPLA (50% starch) membranes were made with compressive moulding. Consequently, the microchannel structures (width 102 μm, 174 μm intervals) were developed on the composite membranes by means of micro-patterned metal mould(s) and hydraulic pressing. An elastomer poly(dimetylsiloxane) stamp was used to transfer heparin as a biochemical cue over the microchannel surfaces by micro-contact printing (μCP). Toluidine blue staining of developed capillaries and heparin μCP-coated membranes showed that heparin was transferred predominantly over the microchannel surfaces. Fibroblast cell culture over the microchannel-formed and heparin μCP-modified SPCL–HA and SPLA membranes showed distinct growth patterns. In contrast to the uniform cell layer formed on unmodified microchannels, the cells were bridging across the grooves of heparin-printed microchannels. At extended culture periods, the heparin-printed microchannels were covered with a layer of fibroblast cells without cellular ingrowths inside. This study indicated that the topographical pattern could induce an organization of fibroblasts only with the biochemical cue and the cells’ functions can be controlled spatially over the microchannels by using both cues. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06 2011-06-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/20665 |
url |
http://hdl.handle.net/1822/20665 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1932-7005 10.1002/term.368 21604378 http://onlinelibrary.wiley.com/doi/10.1002/term.368/pdf |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132490029334528 |