Development of a canine epidermis equivalent model for evaluation of sensitization

Detalhes bibliográficos
Autor(a) principal: Marques, M
Data de Publicação: 2022
Outros Autores: Nunes, J, Ustymenko, B, Lagoa, Tânia, Fialho, Luísa, Martins, Luís, Burke, Anthony, Souza, E, Filho, César, Craveiro, Alexandre, Costa, Ana, Branco, Sandra, Antunes, Célia
Tipo de documento: Artigo de conferência
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/33739
Resumo: Background Canine atopic dermatitis (cAD) is a genetic-predisposed allergic and pruritic inflammatory skin condition, associated with sensitization to environmental allergens. Keratinocytes can produce several inflammatory mediators, in response to several other host mediators, antigens, and pathogens by virtue of their wide range of surface receptors, some of them dysregulated in cAD individuals. A histotypical cell cultured-derived tissue may be used to replace animal testing and are imperative to avoid armful, drawn-out tests to assess chemicals for their capacity to disrupt skin or cause sensitization. The aim of our study was to develop a histotypical canine epidermis equivalent, that could be used for the assessment of skin irritation and sensitization, useful in studies of cAD pathogeny and pharmacology. Methods Canine keratinocytes progenitor cells were seeded in air-lift culture, using an adapted version of the CELLnTECTM protocol. Irritation and sensitization protocols were adapted from human equivalent validated tests. For histological analysis, samples fixed in neutral-buffered formalin and paraffin sections were routinely processed for biopsies and stained with hematoxylin and eosin. Results A multilayer (3-4 cell-layer thick) of canine keratinocytes was developed in air-lift culture, originating a stratified epidermal-like tissue, confirmed by histological analysis. This epidermal-like tissue exhibited functional characteristics of the normal epidermis, showing an adequate impermeabilization after 0.1% Triton X-100 exposure for 4h. Additionally, the epidermis model responded adequately to the positive [5% SDS, 20% salicylic acid in AOO (acetone and olive oil 4:1)] and negative (PBS and AOO) controls of the irritation and sensitization tests, assessed by the quantification of cellular viability and of the secreted IL-18. Conclusion As predicted, a canine epidermis analog was developed. This is a promising canine epidermis model that can be used for the study of skin-derived cAD triggering factors, and in the development and evaluation of new drugs for topical applications. Acknowledgements: Project co-financed by the European Union Fund – Portugal 2020, Alentejo 2020 – Ref. 03/SI/2017 (ALT20-03-247-FEDER-033578).
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spelling Development of a canine epidermis equivalent model for evaluation of sensitizationDogskinepidermiswound healingchitosanhydrogelsBackground Canine atopic dermatitis (cAD) is a genetic-predisposed allergic and pruritic inflammatory skin condition, associated with sensitization to environmental allergens. Keratinocytes can produce several inflammatory mediators, in response to several other host mediators, antigens, and pathogens by virtue of their wide range of surface receptors, some of them dysregulated in cAD individuals. A histotypical cell cultured-derived tissue may be used to replace animal testing and are imperative to avoid armful, drawn-out tests to assess chemicals for their capacity to disrupt skin or cause sensitization. The aim of our study was to develop a histotypical canine epidermis equivalent, that could be used for the assessment of skin irritation and sensitization, useful in studies of cAD pathogeny and pharmacology. Methods Canine keratinocytes progenitor cells were seeded in air-lift culture, using an adapted version of the CELLnTECTM protocol. Irritation and sensitization protocols were adapted from human equivalent validated tests. For histological analysis, samples fixed in neutral-buffered formalin and paraffin sections were routinely processed for biopsies and stained with hematoxylin and eosin. Results A multilayer (3-4 cell-layer thick) of canine keratinocytes was developed in air-lift culture, originating a stratified epidermal-like tissue, confirmed by histological analysis. This epidermal-like tissue exhibited functional characteristics of the normal epidermis, showing an adequate impermeabilization after 0.1% Triton X-100 exposure for 4h. Additionally, the epidermis model responded adequately to the positive [5% SDS, 20% salicylic acid in AOO (acetone and olive oil 4:1)] and negative (PBS and AOO) controls of the irritation and sensitization tests, assessed by the quantification of cellular viability and of the secreted IL-18. Conclusion As predicted, a canine epidermis analog was developed. This is a promising canine epidermis model that can be used for the study of skin-derived cAD triggering factors, and in the development and evaluation of new drugs for topical applications. Acknowledgements: Project co-financed by the European Union Fund – Portugal 2020, Alentejo 2020 – Ref. 03/SI/2017 (ALT20-03-247-FEDER-033578).European Academy of Allergy and Clinical Immunology2023-01-30T17:11:04Z2023-01-302022-07-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/33739http://hdl.handle.net/10174/33739porMarques M, Nunes J, Ustymenko B, Lagoa, T, Fialho L, Martins L, Burke A, Souza EF, Filho CMC, Craveiro AC, Costa AR, Branco S, Antunes CM. Development of a canine epidermis equivalent model for evaluation of sensitization. P1693. ePoster Dermatology. EAACI Hybrid Congress 2022, 1-3 July 2022.https://eaaci.org/events_congress/eaaci-hybrid-congress-2022/simnaosimndndndtsdlagoa@gmail.comlfialho@uevora.ptlmlm@uevora.ptuc46859@uc.ptesmar.idt@brinova.eucesarfilho.idt@gmail.comaccraveiro@hotmail.comacrc@uevora.ptsmbb@uevora.ptcmma@uevora.ptMarques, MNunes, JUstymenko, BLagoa, TâniaFialho, LuísaMartins, LuísBurke, AnthonySouza, EFilho, CésarCraveiro, AlexandreCosta, AnaBranco, SandraAntunes, Céliainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:35:42Zoai:dspace.uevora.pt:10174/33739Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:22:29.060136Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Development of a canine epidermis equivalent model for evaluation of sensitization
title Development of a canine epidermis equivalent model for evaluation of sensitization
spellingShingle Development of a canine epidermis equivalent model for evaluation of sensitization
Marques, M
Dog
skin
epidermis
wound healing
chitosan
hydrogels
title_short Development of a canine epidermis equivalent model for evaluation of sensitization
title_full Development of a canine epidermis equivalent model for evaluation of sensitization
title_fullStr Development of a canine epidermis equivalent model for evaluation of sensitization
title_full_unstemmed Development of a canine epidermis equivalent model for evaluation of sensitization
title_sort Development of a canine epidermis equivalent model for evaluation of sensitization
author Marques, M
author_facet Marques, M
Nunes, J
Ustymenko, B
Lagoa, Tânia
Fialho, Luísa
Martins, Luís
Burke, Anthony
Souza, E
Filho, César
Craveiro, Alexandre
Costa, Ana
Branco, Sandra
Antunes, Célia
author_role author
author2 Nunes, J
Ustymenko, B
Lagoa, Tânia
Fialho, Luísa
Martins, Luís
Burke, Anthony
Souza, E
Filho, César
Craveiro, Alexandre
Costa, Ana
Branco, Sandra
Antunes, Célia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Marques, M
Nunes, J
Ustymenko, B
Lagoa, Tânia
Fialho, Luísa
Martins, Luís
Burke, Anthony
Souza, E
Filho, César
Craveiro, Alexandre
Costa, Ana
Branco, Sandra
Antunes, Célia
dc.subject.por.fl_str_mv Dog
skin
epidermis
wound healing
chitosan
hydrogels
topic Dog
skin
epidermis
wound healing
chitosan
hydrogels
description Background Canine atopic dermatitis (cAD) is a genetic-predisposed allergic and pruritic inflammatory skin condition, associated with sensitization to environmental allergens. Keratinocytes can produce several inflammatory mediators, in response to several other host mediators, antigens, and pathogens by virtue of their wide range of surface receptors, some of them dysregulated in cAD individuals. A histotypical cell cultured-derived tissue may be used to replace animal testing and are imperative to avoid armful, drawn-out tests to assess chemicals for their capacity to disrupt skin or cause sensitization. The aim of our study was to develop a histotypical canine epidermis equivalent, that could be used for the assessment of skin irritation and sensitization, useful in studies of cAD pathogeny and pharmacology. Methods Canine keratinocytes progenitor cells were seeded in air-lift culture, using an adapted version of the CELLnTECTM protocol. Irritation and sensitization protocols were adapted from human equivalent validated tests. For histological analysis, samples fixed in neutral-buffered formalin and paraffin sections were routinely processed for biopsies and stained with hematoxylin and eosin. Results A multilayer (3-4 cell-layer thick) of canine keratinocytes was developed in air-lift culture, originating a stratified epidermal-like tissue, confirmed by histological analysis. This epidermal-like tissue exhibited functional characteristics of the normal epidermis, showing an adequate impermeabilization after 0.1% Triton X-100 exposure for 4h. Additionally, the epidermis model responded adequately to the positive [5% SDS, 20% salicylic acid in AOO (acetone and olive oil 4:1)] and negative (PBS and AOO) controls of the irritation and sensitization tests, assessed by the quantification of cellular viability and of the secreted IL-18. Conclusion As predicted, a canine epidermis analog was developed. This is a promising canine epidermis model that can be used for the study of skin-derived cAD triggering factors, and in the development and evaluation of new drugs for topical applications. Acknowledgements: Project co-financed by the European Union Fund – Portugal 2020, Alentejo 2020 – Ref. 03/SI/2017 (ALT20-03-247-FEDER-033578).
publishDate 2022
dc.date.none.fl_str_mv 2022-07-02T00:00:00Z
2023-01-30T17:11:04Z
2023-01-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/conferenceObject
format conferenceObject
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/33739
http://hdl.handle.net/10174/33739
url http://hdl.handle.net/10174/33739
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Marques M, Nunes J, Ustymenko B, Lagoa, T, Fialho L, Martins L, Burke A, Souza EF, Filho CMC, Craveiro AC, Costa AR, Branco S, Antunes CM. Development of a canine epidermis equivalent model for evaluation of sensitization. P1693. ePoster Dermatology. EAACI Hybrid Congress 2022, 1-3 July 2022.
https://eaaci.org/events_congress/eaaci-hybrid-congress-2022/
sim
nao
sim
nd
nd
nd
tsdlagoa@gmail.com
lfialho@uevora.pt
lmlm@uevora.pt
uc46859@uc.pt
esmar.idt@brinova.eu
cesarfilho.idt@gmail.com
accraveiro@hotmail.com
acrc@uevora.pt
smbb@uevora.pt
cmma@uevora.pt
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv European Academy of Allergy and Clinical Immunology
publisher.none.fl_str_mv European Academy of Allergy and Clinical Immunology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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