COVID-19 salivary protein profile: unravelling molecular aspects of SARS-CoV-2 infection
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/39023 |
Resumo: | COVID-19 is the most impacting global pandemic of all time, with over 600 million infected and 6.5 million deaths worldwide, in addition to an unprecedented economic impact. Despite the many advances in scientific knowledge about the disease, much remains to be clarified about the molecular alterations induced by SARS-CoV-2 infection. In this work, we present a hybrid proteomics and in silico interactomics strategy to establish a COVID-19 salivary protein profile. Data are available via ProteomeXchange with identifier PXD036571. The differential proteome was narrowed down by the Partial Least-Squares Discriminant Analysis and enrichment analysis was performed with FunRich. In parallel, OralInt was used to determine interspecies Protein-Protein Interactions between humans and SARS-CoV-2. Five dysregulated biological processes were identified in the COVID-19 proteome profile: Apoptosis, Energy Pathways, Immune Response, Protein Metabolism and Transport. We identified 10 proteins (KLK 11, IMPA2, ANXA7, PLP2, IGLV2-11, IGHV3-43D, IGKV2-24, TMEM165, VSIG10 and PHB2) that had never been associated with SARS-CoV-2 infection, representing new evidence of the impact of COVID-19. Interactomics analysis showed viral influence on the host immune response, mainly through interaction with the degranulation of neutrophils. The virus alters the host’s energy metabolism and interferes with apoptosis mechanisms. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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COVID-19 salivary protein profile: unravelling molecular aspects of SARS-CoV-2 infectionCOVID-19SalivaProteomicsInteractomicsOralintCOVID-19 is the most impacting global pandemic of all time, with over 600 million infected and 6.5 million deaths worldwide, in addition to an unprecedented economic impact. Despite the many advances in scientific knowledge about the disease, much remains to be clarified about the molecular alterations induced by SARS-CoV-2 infection. In this work, we present a hybrid proteomics and in silico interactomics strategy to establish a COVID-19 salivary protein profile. Data are available via ProteomeXchange with identifier PXD036571. The differential proteome was narrowed down by the Partial Least-Squares Discriminant Analysis and enrichment analysis was performed with FunRich. In parallel, OralInt was used to determine interspecies Protein-Protein Interactions between humans and SARS-CoV-2. Five dysregulated biological processes were identified in the COVID-19 proteome profile: Apoptosis, Energy Pathways, Immune Response, Protein Metabolism and Transport. We identified 10 proteins (KLK 11, IMPA2, ANXA7, PLP2, IGLV2-11, IGHV3-43D, IGKV2-24, TMEM165, VSIG10 and PHB2) that had never been associated with SARS-CoV-2 infection, representing new evidence of the impact of COVID-19. Interactomics analysis showed viral influence on the host immune response, mainly through interaction with the degranulation of neutrophils. The virus alters the host’s energy metabolism and interferes with apoptosis mechanisms.Veritati - Repositório Institucional da Universidade Católica PortuguesaEsteves, EduardoMendes, Vera M.Manadas, BrunoLopes, RafaelaBernardino, LilianaCorreia, Maria JoséBarros, MarleneEsteves, Ana CristinaRosa, Nuno2022-09-28T10:15:56Z2022-09-222022-09-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/39023eng2077-038310.3390/jcm111955718513976742736233441000868033900001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:44:30ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
COVID-19 salivary protein profile: unravelling molecular aspects of SARS-CoV-2 infection |
title |
COVID-19 salivary protein profile: unravelling molecular aspects of SARS-CoV-2 infection |
spellingShingle |
COVID-19 salivary protein profile: unravelling molecular aspects of SARS-CoV-2 infection Esteves, Eduardo COVID-19 Saliva Proteomics Interactomics Oralint |
title_short |
COVID-19 salivary protein profile: unravelling molecular aspects of SARS-CoV-2 infection |
title_full |
COVID-19 salivary protein profile: unravelling molecular aspects of SARS-CoV-2 infection |
title_fullStr |
COVID-19 salivary protein profile: unravelling molecular aspects of SARS-CoV-2 infection |
title_full_unstemmed |
COVID-19 salivary protein profile: unravelling molecular aspects of SARS-CoV-2 infection |
title_sort |
COVID-19 salivary protein profile: unravelling molecular aspects of SARS-CoV-2 infection |
author |
Esteves, Eduardo |
author_facet |
Esteves, Eduardo Mendes, Vera M. Manadas, Bruno Lopes, Rafaela Bernardino, Liliana Correia, Maria José Barros, Marlene Esteves, Ana Cristina Rosa, Nuno |
author_role |
author |
author2 |
Mendes, Vera M. Manadas, Bruno Lopes, Rafaela Bernardino, Liliana Correia, Maria José Barros, Marlene Esteves, Ana Cristina Rosa, Nuno |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Esteves, Eduardo Mendes, Vera M. Manadas, Bruno Lopes, Rafaela Bernardino, Liliana Correia, Maria José Barros, Marlene Esteves, Ana Cristina Rosa, Nuno |
dc.subject.por.fl_str_mv |
COVID-19 Saliva Proteomics Interactomics Oralint |
topic |
COVID-19 Saliva Proteomics Interactomics Oralint |
description |
COVID-19 is the most impacting global pandemic of all time, with over 600 million infected and 6.5 million deaths worldwide, in addition to an unprecedented economic impact. Despite the many advances in scientific knowledge about the disease, much remains to be clarified about the molecular alterations induced by SARS-CoV-2 infection. In this work, we present a hybrid proteomics and in silico interactomics strategy to establish a COVID-19 salivary protein profile. Data are available via ProteomeXchange with identifier PXD036571. The differential proteome was narrowed down by the Partial Least-Squares Discriminant Analysis and enrichment analysis was performed with FunRich. In parallel, OralInt was used to determine interspecies Protein-Protein Interactions between humans and SARS-CoV-2. Five dysregulated biological processes were identified in the COVID-19 proteome profile: Apoptosis, Energy Pathways, Immune Response, Protein Metabolism and Transport. We identified 10 proteins (KLK 11, IMPA2, ANXA7, PLP2, IGLV2-11, IGHV3-43D, IGKV2-24, TMEM165, VSIG10 and PHB2) that had never been associated with SARS-CoV-2 infection, representing new evidence of the impact of COVID-19. Interactomics analysis showed viral influence on the host immune response, mainly through interaction with the degranulation of neutrophils. The virus alters the host’s energy metabolism and interferes with apoptosis mechanisms. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-09-28T10:15:56Z 2022-09-22 2022-09-22T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/39023 |
url |
http://hdl.handle.net/10400.14/39023 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2077-0383 10.3390/jcm11195571 85139767427 36233441 000868033900001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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1777303226231554048 |