Microencapsulation of antitubercular drugs in a matrix of partially hydrolyzed guar gum, for application in tuberculosis treatment

Detalhes bibliográficos
Autor(a) principal: Martins, Tatiana dos Reis
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/43588
Resumo: Trabalho realizado na Universidade do Algarve
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spelling Microencapsulation of antitubercular drugs in a matrix of partially hydrolyzed guar gum, for application in tuberculosis treatmentGuar gummacrophage targetingmicroparticlesspray-dryingTrabalho realizado na Universidade do AlgarveTuberculosis (TB), despite being a completely curable disease, has reemerged due to drug resistance and deadly synergism with HIV infection, which limit the success of its management. Lung tuberculosis is the main manifestation of TB. Thus, exploring the inhalable route for a local delivery of antitubercular drugs seems a promising therapeutic approach. Partially hydrolyzed guar gum (PHGG) is a strong candidate as matrix material for antitubercular drug carriers. This is mainly due to its affinity for macrophages, the hosts of mycobacteria, which is mediated by the binding of sugar units to macrophage surface receptors. In this work, PHGG-based microparticle formulations were produced by spray-drying, evaluated for cristallinity pattern (X-ray diffraction) and ability for drug association, and in vitro drug release profiles were determined. The cytotoxicity of microparticles was also evaluated (MTT and LDH release assays). Additionally, the therapeutic effect of drug-loaded microparticles was evaluated in vitro on macrophage-like cells infected with mycobacteria strains. The results showed that microparticles exhibited suitable properties for pulmonary delivery (aerodynamic diameter between 1 and 3 μm). A favorable cytotoxic profile was evidenced, as no overt toxicity was detected in representative respiratory cell lines (A549 and Calu-3 cells), although a mild toxic effect was observed in macrophage-like cells. The in vitro response of infected macrophages to drug-loaded PHGG microparticles was considered promising, as only 20% of mycobacteria remained viable upon a single treatment with microparticles. This thesis addresses macrophages as therapeutic target, unraveling the unique role of polysaccharides on pulmonary drug delivery in the ambit of tuberculosis therapy.Instituto de Tecnologia Química e Biológica António Xavier. Universidade Nova de LisboaRUNMartins, Tatiana dos Reis2018-08-07T16:36:39Z2015-122015-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/43588TID:202247554enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:23:40Zoai:run.unl.pt:10362/43588Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:31:44.048193Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Microencapsulation of antitubercular drugs in a matrix of partially hydrolyzed guar gum, for application in tuberculosis treatment
title Microencapsulation of antitubercular drugs in a matrix of partially hydrolyzed guar gum, for application in tuberculosis treatment
spellingShingle Microencapsulation of antitubercular drugs in a matrix of partially hydrolyzed guar gum, for application in tuberculosis treatment
Martins, Tatiana dos Reis
Guar gum
macrophage targeting
microparticles
spray-drying
title_short Microencapsulation of antitubercular drugs in a matrix of partially hydrolyzed guar gum, for application in tuberculosis treatment
title_full Microencapsulation of antitubercular drugs in a matrix of partially hydrolyzed guar gum, for application in tuberculosis treatment
title_fullStr Microencapsulation of antitubercular drugs in a matrix of partially hydrolyzed guar gum, for application in tuberculosis treatment
title_full_unstemmed Microencapsulation of antitubercular drugs in a matrix of partially hydrolyzed guar gum, for application in tuberculosis treatment
title_sort Microencapsulation of antitubercular drugs in a matrix of partially hydrolyzed guar gum, for application in tuberculosis treatment
author Martins, Tatiana dos Reis
author_facet Martins, Tatiana dos Reis
author_role author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Martins, Tatiana dos Reis
dc.subject.por.fl_str_mv Guar gum
macrophage targeting
microparticles
spray-drying
topic Guar gum
macrophage targeting
microparticles
spray-drying
description Trabalho realizado na Universidade do Algarve
publishDate 2015
dc.date.none.fl_str_mv 2015-12
2015-12-01T00:00:00Z
2018-08-07T16:36:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/43588
TID:202247554
url http://hdl.handle.net/10362/43588
identifier_str_mv TID:202247554
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier. Universidade Nova de Lisboa
publisher.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier. Universidade Nova de Lisboa
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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