Factors affecting microbial lipids production by Yarrowia lipolytica strains from volatile fatty acids: effect of co-substrates, operation mode and oxygen
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/70926 |
Resumo: | Volatile fatty acids (VFAs), which can be generated by acidogenesis of organic wastes, are important building blocks for chemicals production, and are intermediates in many bioprocesses such as microbial lipids production. Important factors affecting the bioconversion of VFAs (acetate, propionate and butyrate) by Yarrowia lipolytica W29 and NCYC 2904 for growth and lipids accumulation were studied. Yarrowia lipolytica grew efficiently in VFAs-based media, but lipids production was enhanced by the addition of co-substrates (glucose or glycerol) in batch cultures. A two-stage batch culture growth phase on glucose, followed by VFAs addition improved lipids accumulation. Lipids concentrations of 2.3?g·L-1 and 3.5?g·L-1 were obtained with this mode of operation, with addition of 18?g·L-1 VFAs, for Y. lipolytica W29 and NCYC 2904, respectively. For the first time, it was demonstrated that oxygen mass transfer is a crucial factor for lipids production by Y. lipolytica from VFAs. Intracellular lipids produced by Y. lipolytica strains were mainly composed by oleic and linoleic acids, similar to common vegetable oils, making these lipids suitable for biodiesel production. Moreover, margaric acid, which may improve biodiesel properties, was only detected in propionate medium. The strategies studied herein will contribute to the feasibility of using VFAs as low-cost feedstock for microbial lipids production by Y. lipolytica strains. |
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Factors affecting microbial lipids production by Yarrowia lipolytica strains from volatile fatty acids: effect of co-substrates, operation mode and oxygenYarrowia lipolyticavolatile fatty acidsacetatepropionatebutyratemicrobial lipidsScience & TechnologyVolatile fatty acids (VFAs), which can be generated by acidogenesis of organic wastes, are important building blocks for chemicals production, and are intermediates in many bioprocesses such as microbial lipids production. Important factors affecting the bioconversion of VFAs (acetate, propionate and butyrate) by Yarrowia lipolytica W29 and NCYC 2904 for growth and lipids accumulation were studied. Yarrowia lipolytica grew efficiently in VFAs-based media, but lipids production was enhanced by the addition of co-substrates (glucose or glycerol) in batch cultures. A two-stage batch culture growth phase on glucose, followed by VFAs addition improved lipids accumulation. Lipids concentrations of 2.3?g·L-1 and 3.5?g·L-1 were obtained with this mode of operation, with addition of 18?g·L-1 VFAs, for Y. lipolytica W29 and NCYC 2904, respectively. For the first time, it was demonstrated that oxygen mass transfer is a crucial factor for lipids production by Y. lipolytica from VFAs. Intracellular lipids produced by Y. lipolytica strains were mainly composed by oleic and linoleic acids, similar to common vegetable oils, making these lipids suitable for biodiesel production. Moreover, margaric acid, which may improve biodiesel properties, was only detected in propionate medium. The strategies studied herein will contribute to the feasibility of using VFAs as low-cost feedstock for microbial lipids production by Y. lipolytica strains.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the project TUBITAK 2014 (TUBITAK/0009/2014), the strategic funding of UIDB/04469/2020 unit and Doctoral grants (SFRH/BD/144188/2019; SFRH/BD/129592/2017).info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoPereira, Ana Sofia SilvaMiranda, Sílvia Cristiana MartinsLopes, MarleneBelo, Isabel2021-042021-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/70926engPereira, Ana S.; Miranda, Sílvia M.; Lopes, Marlene; Belo, Isabel, Factors affecting microbial lipids production by Yarrowia lipolytica strains from volatile fatty acids: effect of co-substrates, operation mode and oxygen. Journal of Biotechnology, 331, 37-47, 20210168165610.1016/j.jbiotec.2021.02.01433652072http://www.elsevier.com/locate/issn/01681656info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:05:11Zoai:repositorium.sdum.uminho.pt:1822/70926Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:55:35.108187Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Factors affecting microbial lipids production by Yarrowia lipolytica strains from volatile fatty acids: effect of co-substrates, operation mode and oxygen |
title |
Factors affecting microbial lipids production by Yarrowia lipolytica strains from volatile fatty acids: effect of co-substrates, operation mode and oxygen |
spellingShingle |
Factors affecting microbial lipids production by Yarrowia lipolytica strains from volatile fatty acids: effect of co-substrates, operation mode and oxygen Pereira, Ana Sofia Silva Yarrowia lipolytica volatile fatty acids acetate propionate butyrate microbial lipids Science & Technology |
title_short |
Factors affecting microbial lipids production by Yarrowia lipolytica strains from volatile fatty acids: effect of co-substrates, operation mode and oxygen |
title_full |
Factors affecting microbial lipids production by Yarrowia lipolytica strains from volatile fatty acids: effect of co-substrates, operation mode and oxygen |
title_fullStr |
Factors affecting microbial lipids production by Yarrowia lipolytica strains from volatile fatty acids: effect of co-substrates, operation mode and oxygen |
title_full_unstemmed |
Factors affecting microbial lipids production by Yarrowia lipolytica strains from volatile fatty acids: effect of co-substrates, operation mode and oxygen |
title_sort |
Factors affecting microbial lipids production by Yarrowia lipolytica strains from volatile fatty acids: effect of co-substrates, operation mode and oxygen |
author |
Pereira, Ana Sofia Silva |
author_facet |
Pereira, Ana Sofia Silva Miranda, Sílvia Cristiana Martins Lopes, Marlene Belo, Isabel |
author_role |
author |
author2 |
Miranda, Sílvia Cristiana Martins Lopes, Marlene Belo, Isabel |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Pereira, Ana Sofia Silva Miranda, Sílvia Cristiana Martins Lopes, Marlene Belo, Isabel |
dc.subject.por.fl_str_mv |
Yarrowia lipolytica volatile fatty acids acetate propionate butyrate microbial lipids Science & Technology |
topic |
Yarrowia lipolytica volatile fatty acids acetate propionate butyrate microbial lipids Science & Technology |
description |
Volatile fatty acids (VFAs), which can be generated by acidogenesis of organic wastes, are important building blocks for chemicals production, and are intermediates in many bioprocesses such as microbial lipids production. Important factors affecting the bioconversion of VFAs (acetate, propionate and butyrate) by Yarrowia lipolytica W29 and NCYC 2904 for growth and lipids accumulation were studied. Yarrowia lipolytica grew efficiently in VFAs-based media, but lipids production was enhanced by the addition of co-substrates (glucose or glycerol) in batch cultures. A two-stage batch culture growth phase on glucose, followed by VFAs addition improved lipids accumulation. Lipids concentrations of 2.3?g·L-1 and 3.5?g·L-1 were obtained with this mode of operation, with addition of 18?g·L-1 VFAs, for Y. lipolytica W29 and NCYC 2904, respectively. For the first time, it was demonstrated that oxygen mass transfer is a crucial factor for lipids production by Y. lipolytica from VFAs. Intracellular lipids produced by Y. lipolytica strains were mainly composed by oleic and linoleic acids, similar to common vegetable oils, making these lipids suitable for biodiesel production. Moreover, margaric acid, which may improve biodiesel properties, was only detected in propionate medium. The strategies studied herein will contribute to the feasibility of using VFAs as low-cost feedstock for microbial lipids production by Y. lipolytica strains. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04 2021-04-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/70926 |
url |
http://hdl.handle.net/1822/70926 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pereira, Ana S.; Miranda, Sílvia M.; Lopes, Marlene; Belo, Isabel, Factors affecting microbial lipids production by Yarrowia lipolytica strains from volatile fatty acids: effect of co-substrates, operation mode and oxygen. Journal of Biotechnology, 331, 37-47, 2021 01681656 10.1016/j.jbiotec.2021.02.014 33652072 http://www.elsevier.com/locate/issn/01681656 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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