The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/74 |
Resumo: | Recently we described the novel nodulin gene VfENOD18, whose corresponding transcripts were restricted to the nitrogen-fixing zone III of broad bean root nodules. To characterize VfENOD18 on the protein level, polyclonal antibodies were generated using the purified recombinant VfENOD18 protein produced in Escherichia coli by employing the pMAL-c expression system. These antibodies recognized immunoreactive proteins isolated from indeterminate nodules of different leguminous plants, but also from non-symbiotic tissues of Glycine max and from tissues of Arabidopsis thaliana and Zea mays. Using immunogold labelling the nodulin VfENOD18 was localized to the cytoplasm of infected cells in the nitrogen-fixing zone of broad bean nodules. Due to the homology of the VfENOD18 sequence to that of the ATP-binding protein MJ0577 from the hyperthermophile Methanococcus jannaschii the recombinant VfENOD18 protein was tested for ATP-binding. Using the biotin photoaffinity ATP analogue 8N3ATP[gamma]biotin it could be demonstrated that VfENOD18 is an ATP-binding protein. PCR experiments revealed that the amino acid sequences of the putative C-terminal ATP-binding sites of the VfENOD 18 homologues from Lens culinaris, Vicia hirsuta, Vicia sativa and Vicia villosa were conserved. We propose that VfENOD18 is a member of a novel family of ATP-binding proteins in plants. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodulesAdenosine Triphosphate/metabolismFabaceae/cytology/genetics/microbiologyMembrane ProteinsPlant Proteins/genetics/immunology/metabolismPlant Roots/genetics/microbiology/ultrastructurebroad beanheterologous expressionMJ0577 superfamilynitrogen fixationnodule-specific gene expressionRecently we described the novel nodulin gene VfENOD18, whose corresponding transcripts were restricted to the nitrogen-fixing zone III of broad bean root nodules. To characterize VfENOD18 on the protein level, polyclonal antibodies were generated using the purified recombinant VfENOD18 protein produced in Escherichia coli by employing the pMAL-c expression system. These antibodies recognized immunoreactive proteins isolated from indeterminate nodules of different leguminous plants, but also from non-symbiotic tissues of Glycine max and from tissues of Arabidopsis thaliana and Zea mays. Using immunogold labelling the nodulin VfENOD18 was localized to the cytoplasm of infected cells in the nitrogen-fixing zone of broad bean nodules. Due to the homology of the VfENOD18 sequence to that of the ATP-binding protein MJ0577 from the hyperthermophile Methanococcus jannaschii the recombinant VfENOD18 protein was tested for ATP-binding. Using the biotin photoaffinity ATP analogue 8N3ATP[gamma]biotin it could be demonstrated that VfENOD18 is an ATP-binding protein. PCR experiments revealed that the amino acid sequences of the putative C-terminal ATP-binding sites of the VfENOD 18 homologues from Lens culinaris, Vicia hirsuta, Vicia sativa and Vicia villosa were conserved. We propose that VfENOD18 is a member of a novel family of ATP-binding proteins in plants.SpringerARCABecker, J.D.Moreira, L.M.Kapp, D.Frosch S.C.Pühler A.Perlick, A.M.2010-03-19T11:29:46Z2001-122001-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/74engBecker, J.D, Moreira LM, Kapp D, Frosch SC, Pühler A, Perlick AM (2001). The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules. Plant Molecular Biology. 47 :749-759ISSN:0167-4412info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:34:37ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules |
title |
The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules |
spellingShingle |
The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules Becker, J.D. Adenosine Triphosphate/metabolism Fabaceae/cytology/genetics/microbiology Membrane Proteins Plant Proteins/genetics/immunology/metabolism Plant Roots/genetics/microbiology/ultrastructure broad bean heterologous expression MJ0577 superfamily nitrogen fixation nodule-specific gene expression |
title_short |
The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules |
title_full |
The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules |
title_fullStr |
The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules |
title_full_unstemmed |
The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules |
title_sort |
The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules |
author |
Becker, J.D. |
author_facet |
Becker, J.D. Moreira, L.M. Kapp, D. Frosch S.C. Pühler A. Perlick, A.M. |
author_role |
author |
author2 |
Moreira, L.M. Kapp, D. Frosch S.C. Pühler A. Perlick, A.M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Becker, J.D. Moreira, L.M. Kapp, D. Frosch S.C. Pühler A. Perlick, A.M. |
dc.subject.por.fl_str_mv |
Adenosine Triphosphate/metabolism Fabaceae/cytology/genetics/microbiology Membrane Proteins Plant Proteins/genetics/immunology/metabolism Plant Roots/genetics/microbiology/ultrastructure broad bean heterologous expression MJ0577 superfamily nitrogen fixation nodule-specific gene expression |
topic |
Adenosine Triphosphate/metabolism Fabaceae/cytology/genetics/microbiology Membrane Proteins Plant Proteins/genetics/immunology/metabolism Plant Roots/genetics/microbiology/ultrastructure broad bean heterologous expression MJ0577 superfamily nitrogen fixation nodule-specific gene expression |
description |
Recently we described the novel nodulin gene VfENOD18, whose corresponding transcripts were restricted to the nitrogen-fixing zone III of broad bean root nodules. To characterize VfENOD18 on the protein level, polyclonal antibodies were generated using the purified recombinant VfENOD18 protein produced in Escherichia coli by employing the pMAL-c expression system. These antibodies recognized immunoreactive proteins isolated from indeterminate nodules of different leguminous plants, but also from non-symbiotic tissues of Glycine max and from tissues of Arabidopsis thaliana and Zea mays. Using immunogold labelling the nodulin VfENOD18 was localized to the cytoplasm of infected cells in the nitrogen-fixing zone of broad bean nodules. Due to the homology of the VfENOD18 sequence to that of the ATP-binding protein MJ0577 from the hyperthermophile Methanococcus jannaschii the recombinant VfENOD18 protein was tested for ATP-binding. Using the biotin photoaffinity ATP analogue 8N3ATP[gamma]biotin it could be demonstrated that VfENOD18 is an ATP-binding protein. PCR experiments revealed that the amino acid sequences of the putative C-terminal ATP-binding sites of the VfENOD 18 homologues from Lens culinaris, Vicia hirsuta, Vicia sativa and Vicia villosa were conserved. We propose that VfENOD18 is a member of a novel family of ATP-binding proteins in plants. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-12 2001-12-01T00:00:00Z 2010-03-19T11:29:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/74 |
url |
http://hdl.handle.net/10400.7/74 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Becker, J.D, Moreira LM, Kapp D, Frosch SC, Pühler A, Perlick AM (2001). The nodulin VfENOD18 is an ATP-binding protein in infected cells of Vicia faba L. nodules. Plant Molecular Biology. 47 :749-759 ISSN:0167-4412 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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