Polyphasic, including MALDI-TOF MS, evaluation of freeze-drying long-term preservation on Aspergillus (section Nigri) strains
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/61259 |
Resumo: | This study aims to evaluate the effect of freeze-drying and long-term storage on the biotechnological potential of Aspergillus section Nigri strains. Twelve selected strains were freeze-dried and aged by accelerated storage, at 37 °C in the dark, for 2 and 4 weeks. To assess possible changes as a consequence of the ageing in the freeze-drying ampoules, morphological characteristics, mycotoxins and enzymes production, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALTI-TOF MS) spectra, and M13 phage probe fingerprinting were used as part of a polyphasic approach. Phenotypical changes were observed; nevertheless, they did not substantially affect the potential biotechnological use of these strains. The activity of hydrolytic enzymes (protease, carboxymethylcellulase, xylanase, pectinase and mannanase) was maintained or increased after freeze-drying. MALDI-TOF MS data originated spectra that grouped, for the majority of samples, according to strain independently of preservation time point. M13 profiles revealed the presence of some genetic polymorphisms after preservation. However, the three studied times still clustered for more than 50% of strains. Our results show that the studied strains maintain their biotechnological potential after preservation, with minimal phenotypic alterations. These findings provide evidence that freeze-drying preservation is a suitable option to preserve biotechnologically relevant aspergilli strains from section Nigri, and one should consider that the observed effects might be species/strain-dependent. |
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Polyphasic, including MALDI-TOF MS, evaluation of freeze-drying long-term preservation on Aspergillus (section Nigri) strainsfreeze-dryingaccelerated ageingmass spectrometryDNA fingerprintingbiotechnological potentialScience & TechnologyThis study aims to evaluate the effect of freeze-drying and long-term storage on the biotechnological potential of Aspergillus section Nigri strains. Twelve selected strains were freeze-dried and aged by accelerated storage, at 37 °C in the dark, for 2 and 4 weeks. To assess possible changes as a consequence of the ageing in the freeze-drying ampoules, morphological characteristics, mycotoxins and enzymes production, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALTI-TOF MS) spectra, and M13 phage probe fingerprinting were used as part of a polyphasic approach. Phenotypical changes were observed; nevertheless, they did not substantially affect the potential biotechnological use of these strains. The activity of hydrolytic enzymes (protease, carboxymethylcellulase, xylanase, pectinase and mannanase) was maintained or increased after freeze-drying. MALDI-TOF MS data originated spectra that grouped, for the majority of samples, according to strain independently of preservation time point. M13 profiles revealed the presence of some genetic polymorphisms after preservation. However, the three studied times still clustered for more than 50% of strains. Our results show that the studied strains maintain their biotechnological potential after preservation, with minimal phenotypic alterations. These findings provide evidence that freeze-drying preservation is a suitable option to preserve biotechnologically relevant aspergilli strains from section Nigri, and one should consider that the observed effects might be species/strain-dependent.This research was funded by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—ProgramaOperacionalRegionaldoNorte. Cl.S.(CledirSantos) thanks the Universidad de La Frontera (Temuco, Chile) for the partial funding from the Project DIUFRO PIA19-0001. R.R. thanks to CONICYT/Chile for the MSc grant Folio N◦ 7317076, Application N◦ 73170764.info:eu-repo/semantics/publishedVersionMDPI PublishingUniversidade do MinhoRodriguez, R.Santos, CarlaSimões, Marta FilipaSoares, CéliaSantos, CledirLima, Nelson20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/61259engRodriguez, R.; Santos, Carla; Simões, Marta Filipa; Soares, Célia; Santos, Cledir; Lima, Nelson, Polyphasic, including MALDI-TOF MS, evaluation of freeze-drying long-term preservation on Aspergillus (section Nigri) strains. Microorganisms, 7(9), 291, 20192076-26072076-260710.3390/microorganisms7090291http://www.mdpi.com/journal/microorganismsinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:15:19Zoai:repositorium.sdum.uminho.pt:1822/61259Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:07:42.944027Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Polyphasic, including MALDI-TOF MS, evaluation of freeze-drying long-term preservation on Aspergillus (section Nigri) strains |
title |
Polyphasic, including MALDI-TOF MS, evaluation of freeze-drying long-term preservation on Aspergillus (section Nigri) strains |
spellingShingle |
Polyphasic, including MALDI-TOF MS, evaluation of freeze-drying long-term preservation on Aspergillus (section Nigri) strains Rodriguez, R. freeze-drying accelerated ageing mass spectrometry DNA fingerprinting biotechnological potential Science & Technology |
title_short |
Polyphasic, including MALDI-TOF MS, evaluation of freeze-drying long-term preservation on Aspergillus (section Nigri) strains |
title_full |
Polyphasic, including MALDI-TOF MS, evaluation of freeze-drying long-term preservation on Aspergillus (section Nigri) strains |
title_fullStr |
Polyphasic, including MALDI-TOF MS, evaluation of freeze-drying long-term preservation on Aspergillus (section Nigri) strains |
title_full_unstemmed |
Polyphasic, including MALDI-TOF MS, evaluation of freeze-drying long-term preservation on Aspergillus (section Nigri) strains |
title_sort |
Polyphasic, including MALDI-TOF MS, evaluation of freeze-drying long-term preservation on Aspergillus (section Nigri) strains |
author |
Rodriguez, R. |
author_facet |
Rodriguez, R. Santos, Carla Simões, Marta Filipa Soares, Célia Santos, Cledir Lima, Nelson |
author_role |
author |
author2 |
Santos, Carla Simões, Marta Filipa Soares, Célia Santos, Cledir Lima, Nelson |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Rodriguez, R. Santos, Carla Simões, Marta Filipa Soares, Célia Santos, Cledir Lima, Nelson |
dc.subject.por.fl_str_mv |
freeze-drying accelerated ageing mass spectrometry DNA fingerprinting biotechnological potential Science & Technology |
topic |
freeze-drying accelerated ageing mass spectrometry DNA fingerprinting biotechnological potential Science & Technology |
description |
This study aims to evaluate the effect of freeze-drying and long-term storage on the biotechnological potential of Aspergillus section Nigri strains. Twelve selected strains were freeze-dried and aged by accelerated storage, at 37 °C in the dark, for 2 and 4 weeks. To assess possible changes as a consequence of the ageing in the freeze-drying ampoules, morphological characteristics, mycotoxins and enzymes production, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALTI-TOF MS) spectra, and M13 phage probe fingerprinting were used as part of a polyphasic approach. Phenotypical changes were observed; nevertheless, they did not substantially affect the potential biotechnological use of these strains. The activity of hydrolytic enzymes (protease, carboxymethylcellulase, xylanase, pectinase and mannanase) was maintained or increased after freeze-drying. MALDI-TOF MS data originated spectra that grouped, for the majority of samples, according to strain independently of preservation time point. M13 profiles revealed the presence of some genetic polymorphisms after preservation. However, the three studied times still clustered for more than 50% of strains. Our results show that the studied strains maintain their biotechnological potential after preservation, with minimal phenotypic alterations. These findings provide evidence that freeze-drying preservation is a suitable option to preserve biotechnologically relevant aspergilli strains from section Nigri, and one should consider that the observed effects might be species/strain-dependent. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/61259 |
url |
http://hdl.handle.net/1822/61259 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Rodriguez, R.; Santos, Carla; Simões, Marta Filipa; Soares, Célia; Santos, Cledir; Lima, Nelson, Polyphasic, including MALDI-TOF MS, evaluation of freeze-drying long-term preservation on Aspergillus (section Nigri) strains. Microorganisms, 7(9), 291, 2019 2076-2607 2076-2607 10.3390/microorganisms7090291 http://www.mdpi.com/journal/microorganisms |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI Publishing |
publisher.none.fl_str_mv |
MDPI Publishing |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132497229905920 |