Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water
Autor(a) principal: | |
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Data de Publicação: | 1996 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/8138 https://doi.org/10.1111/j.1365-2672.1996.tb03246.x |
Resumo: | The microflora of a natural mineral water was studied immediately after bottling (T0) and after 7 d storage (T7) during 6 months, and isolates were clustered by SDS-PAGE of wholecell protein profiles. Isolates from each cluster were further characterized by API 20NE, fatty acid composition and quinone profiles. The numerical analysis of the electrophoregrams of all bacteria isolated from the mineral water formed 15 clusters and five unclustered strains. Except for five minor clusters, all clusters were composed of strains isolated over several months. The numerical analysis of the electrophoregrams of bacteria isolated immediately after bottling formed 15 clusters while after 7 d storage only four of these populations could be isolated, indicating that populations present in the mineral water were stable and that changes occurring after bottling probably resulted from a selection process. Only one unclustered strain was identified simultaneously by all the systems, as Sphingomonas paucimobilis. The monitoring of the aquifer and the bottling system, and the construction of a large database with bacteria of the autochthonous flora allows the detection of alterations in the aquifer by changes in the microflora. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral waterThe microflora of a natural mineral water was studied immediately after bottling (T0) and after 7 d storage (T7) during 6 months, and isolates were clustered by SDS-PAGE of wholecell protein profiles. Isolates from each cluster were further characterized by API 20NE, fatty acid composition and quinone profiles. The numerical analysis of the electrophoregrams of all bacteria isolated from the mineral water formed 15 clusters and five unclustered strains. Except for five minor clusters, all clusters were composed of strains isolated over several months. The numerical analysis of the electrophoregrams of bacteria isolated immediately after bottling formed 15 clusters while after 7 d storage only four of these populations could be isolated, indicating that populations present in the mineral water were stable and that changes occurring after bottling probably resulted from a selection process. Only one unclustered strain was identified simultaneously by all the systems, as Sphingomonas paucimobilis. The monitoring of the aquifer and the bottling system, and the construction of a large database with bacteria of the autochthonous flora allows the detection of alterations in the aquifer by changes in the microflora.1996info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/8138http://hdl.handle.net/10316/8138https://doi.org/10.1111/j.1365-2672.1996.tb03246.xengJournal of Applied Microbiology. 80:5 (1996) 479-486Ferreira, A. C.Morais, P. V.Gomes, C.Costa, M. S. dainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-05-25T07:21:47ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water |
title |
Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water |
spellingShingle |
Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water Ferreira, A. C. |
title_short |
Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water |
title_full |
Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water |
title_fullStr |
Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water |
title_full_unstemmed |
Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water |
title_sort |
Computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water |
author |
Ferreira, A. C. |
author_facet |
Ferreira, A. C. Morais, P. V. Gomes, C. Costa, M. S. da |
author_role |
author |
author2 |
Morais, P. V. Gomes, C. Costa, M. S. da |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Ferreira, A. C. Morais, P. V. Gomes, C. Costa, M. S. da |
description |
The microflora of a natural mineral water was studied immediately after bottling (T0) and after 7 d storage (T7) during 6 months, and isolates were clustered by SDS-PAGE of wholecell protein profiles. Isolates from each cluster were further characterized by API 20NE, fatty acid composition and quinone profiles. The numerical analysis of the electrophoregrams of all bacteria isolated from the mineral water formed 15 clusters and five unclustered strains. Except for five minor clusters, all clusters were composed of strains isolated over several months. The numerical analysis of the electrophoregrams of bacteria isolated immediately after bottling formed 15 clusters while after 7 d storage only four of these populations could be isolated, indicating that populations present in the mineral water were stable and that changes occurring after bottling probably resulted from a selection process. Only one unclustered strain was identified simultaneously by all the systems, as Sphingomonas paucimobilis. The monitoring of the aquifer and the bottling system, and the construction of a large database with bacteria of the autochthonous flora allows the detection of alterations in the aquifer by changes in the microflora. |
publishDate |
1996 |
dc.date.none.fl_str_mv |
1996 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/8138 http://hdl.handle.net/10316/8138 https://doi.org/10.1111/j.1365-2672.1996.tb03246.x |
url |
http://hdl.handle.net/10316/8138 https://doi.org/10.1111/j.1365-2672.1996.tb03246.x |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Microbiology. 80:5 (1996) 479-486 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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1777302656954400768 |