Inactivation of Pseudomonas aeruginosa in mineral water by DP1 bacteriophage immobilized on ethylene-vinyl acetate copolymer used as seal caps of plastic bottles
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/65723 |
Resumo: | Pseudomonas aeruginosa has been found in bottled natural mineral water, even though its presence is not allowed in this product by different food regulations. This study aimed to investigate the inactivation of P. aeruginosa present in mineral water by vB_PaeM_CEB_DP1 (short name DP1) bacteriophage immobilized on ethylenevinyl acetate (EVA) copolymer used as seal caps of plastic bottles. EVA was chemically modified using microwaveassisted alcoholysis, improving polymerphage binding. After that, DP1 phage was attached to EVA and EVAOH copolymers and both surfaces were tested for plaque formation using P. aeruginosa. Then, both materials containing immobilized phages were used as seal caps of plastic bottles and its antimicrobial capacity was tested against P. aeruginosa contaminating mineral water. The EVAOH resulted in higher hydrogen bond density that contributed significantly to the phage immobilization on the polymer surface. The polymers containing immobilized phages were able to reduce 0.53 log of P. aeruginosa population present inside mineral water bottles after 14days. |
id |
RCAP_1ad54e6873481ff68e7b80f4a0e9493a |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/65723 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Inactivation of Pseudomonas aeruginosa in mineral water by DP1 bacteriophage immobilized on ethylene-vinyl acetate copolymer used as seal caps of plastic bottlesapplicationsfunctionalization of polymerspackagingScience & TechnologyPseudomonas aeruginosa has been found in bottled natural mineral water, even though its presence is not allowed in this product by different food regulations. This study aimed to investigate the inactivation of P. aeruginosa present in mineral water by vB_PaeM_CEB_DP1 (short name DP1) bacteriophage immobilized on ethylenevinyl acetate (EVA) copolymer used as seal caps of plastic bottles. EVA was chemically modified using microwaveassisted alcoholysis, improving polymerphage binding. After that, DP1 phage was attached to EVA and EVAOH copolymers and both surfaces were tested for plaque formation using P. aeruginosa. Then, both materials containing immobilized phages were used as seal caps of plastic bottles and its antimicrobial capacity was tested against P. aeruginosa contaminating mineral water. The EVAOH resulted in higher hydrogen bond density that contributed significantly to the phage immobilization on the polymer surface. The polymers containing immobilized phages were able to reduce 0.53 log of P. aeruginosa population present inside mineral water bottles after 14days.The authors thank CAPES for scholarships to Cesar H. Wanke and Junia Novello. CNPq—National Council for Scientific and Technological Development, Brazil for financial support (grant numbers 308241/2015-0 and 306086/2018-2). Sanna Sillankorva acknowledges funding from the European Union’s Horizon 2020 research and innovation programme (grant number 713640).info:eu-repo/semantics/publishedVersionJohn Wiley & SonsUniversidade do MinhoNovello, JúniaSillankorva, SannaPires, Diana P.Azeredo, JoanaWanke, César HenriqueTondo, Eduardo CésarBianchi, Otávio2020-09-152020-09-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/65723engNovello, Júnia; Sillankorva, Sanna; Pires, Diana P.; Azeredo, Joana; Wanke, César Henrique; Tondo, Eduardo César; Bianchi, Otávio, Inactivation of Pseudomonas aeruginosa in mineral water by DP1 bacteriophage immobilized on ethylene-vinyl acetate copolymer used as seal caps of plastic bottles. Journal of Applied Polymer Science, 137(35), 49009, 20200021-89951097-462810.1002/app.49009http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-4628info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:52:01Zoai:repositorium.sdum.uminho.pt:1822/65723Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:51:03.467294Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Inactivation of Pseudomonas aeruginosa in mineral water by DP1 bacteriophage immobilized on ethylene-vinyl acetate copolymer used as seal caps of plastic bottles |
title |
Inactivation of Pseudomonas aeruginosa in mineral water by DP1 bacteriophage immobilized on ethylene-vinyl acetate copolymer used as seal caps of plastic bottles |
spellingShingle |
Inactivation of Pseudomonas aeruginosa in mineral water by DP1 bacteriophage immobilized on ethylene-vinyl acetate copolymer used as seal caps of plastic bottles Novello, Júnia applications functionalization of polymers packaging Science & Technology |
title_short |
Inactivation of Pseudomonas aeruginosa in mineral water by DP1 bacteriophage immobilized on ethylene-vinyl acetate copolymer used as seal caps of plastic bottles |
title_full |
Inactivation of Pseudomonas aeruginosa in mineral water by DP1 bacteriophage immobilized on ethylene-vinyl acetate copolymer used as seal caps of plastic bottles |
title_fullStr |
Inactivation of Pseudomonas aeruginosa in mineral water by DP1 bacteriophage immobilized on ethylene-vinyl acetate copolymer used as seal caps of plastic bottles |
title_full_unstemmed |
Inactivation of Pseudomonas aeruginosa in mineral water by DP1 bacteriophage immobilized on ethylene-vinyl acetate copolymer used as seal caps of plastic bottles |
title_sort |
Inactivation of Pseudomonas aeruginosa in mineral water by DP1 bacteriophage immobilized on ethylene-vinyl acetate copolymer used as seal caps of plastic bottles |
author |
Novello, Júnia |
author_facet |
Novello, Júnia Sillankorva, Sanna Pires, Diana P. Azeredo, Joana Wanke, César Henrique Tondo, Eduardo César Bianchi, Otávio |
author_role |
author |
author2 |
Sillankorva, Sanna Pires, Diana P. Azeredo, Joana Wanke, César Henrique Tondo, Eduardo César Bianchi, Otávio |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Novello, Júnia Sillankorva, Sanna Pires, Diana P. Azeredo, Joana Wanke, César Henrique Tondo, Eduardo César Bianchi, Otávio |
dc.subject.por.fl_str_mv |
applications functionalization of polymers packaging Science & Technology |
topic |
applications functionalization of polymers packaging Science & Technology |
description |
Pseudomonas aeruginosa has been found in bottled natural mineral water, even though its presence is not allowed in this product by different food regulations. This study aimed to investigate the inactivation of P. aeruginosa present in mineral water by vB_PaeM_CEB_DP1 (short name DP1) bacteriophage immobilized on ethylenevinyl acetate (EVA) copolymer used as seal caps of plastic bottles. EVA was chemically modified using microwaveassisted alcoholysis, improving polymerphage binding. After that, DP1 phage was attached to EVA and EVAOH copolymers and both surfaces were tested for plaque formation using P. aeruginosa. Then, both materials containing immobilized phages were used as seal caps of plastic bottles and its antimicrobial capacity was tested against P. aeruginosa contaminating mineral water. The EVAOH resulted in higher hydrogen bond density that contributed significantly to the phage immobilization on the polymer surface. The polymers containing immobilized phages were able to reduce 0.53 log of P. aeruginosa population present inside mineral water bottles after 14days. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-09-15 2020-09-15T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/65723 |
url |
http://hdl.handle.net/1822/65723 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Novello, Júnia; Sillankorva, Sanna; Pires, Diana P.; Azeredo, Joana; Wanke, César Henrique; Tondo, Eduardo César; Bianchi, Otávio, Inactivation of Pseudomonas aeruginosa in mineral water by DP1 bacteriophage immobilized on ethylene-vinyl acetate copolymer used as seal caps of plastic bottles. Journal of Applied Polymer Science, 137(35), 49009, 2020 0021-8995 1097-4628 10.1002/app.49009 http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-4628 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
John Wiley & Sons |
publisher.none.fl_str_mv |
John Wiley & Sons |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133097495625728 |