Morphological, biochemical and molecular approaches to the identification of Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenaria in Portugal

Detalhes bibliográficos
Autor(a) principal: Rusinque, Leidy Constanza Mora
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/13872
Resumo: Mestrado em Engenharia Agonómica - Proteção de plantas - Instituto Superior de Agronomia - UL
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spelling Morphological, biochemical and molecular approaches to the identification of Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenaria in Portugalroot-knot nematodeMeloidogyne spp.perineal patternsesterase phenotypePCRMestrado em Engenharia Agonómica - Proteção de plantas - Instituto Superior de Agronomia - ULPlant parasitic nematodes are highly damaging pests in many crops of great economic importance. A substantial part of this damage is caused by infestations of root-knot nematodes (RKN), due to their wide geographical distribution as well as a vast range of host plants. The most important and widely distributed root-knot nematode species are Meloidogyne incognita, M. arenaria and M. javanica accounting for almost 95% of the occurrences, and so, an accurate and reliable identification it is primary to establish effective, sustainable and environmentally safe control measures. The main goals of this study were to characterise morphologically, biochemically and molecularly Portuguese isolates and reference material from Netherlands Food and Consumer Product Safety Authority (NVWA), Wageningen, The Netherlands, in order to find the most suitable tools that can be used in the Laboratory of Molecular Genetics and Nematology at the INIAV in Portugal to assist in the identification of the three most common species of Meloidogyne. Morphological studies incorporated the examination of female’s perineal pattern and measurements of second stage juveniles’ features, which presented enough variation to confirm the unreliability and difficulty of morphological identification alone. Biochemical assays with isozymes such as esterases (EST) were performed on females and the PAGE enzymatic patterns obtained match those already described for M. javanica. Molecular analysis included PCRs using universal primers that target the 28S gene, ITS and IGS ribosomal DNA regions (rDNA) and the region between the cytochrome oxidase (COII) of the mitochondrial DNA (mtDNA) and 16S rDNA genes together with speciesspecific SCAR primers and sequencing and cloning. The sequences obtained from sequencing of PCR fragments and from cloned fragments were aligned and compared to those found in GenBank database through BLAST analysis with inconclusive results. Although it was no possible to uncover an efficient tool for Meloidogyne identification this study pinpointed that the three approaches for root-knot nematode identification need to be used if we are to identify this species accurately and effectively. What’s more this research can be used as platform for further studies regarding this species at the Nematology of INIAV PortugalISA/ULNóbrega, Maria Filomena de SousaMota, Mariana GomesRepositório da Universidade de LisboaRusinque, Leidy Constanza Mora2017-07-07T09:46:40Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/13872engRusinque, L.C.M. - Morphological, biochemical and molecular approaches to the identification of Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenaria in Portugal. Lisboa: ISA, 2017, 80 p., 4 apend.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:43:57Zoai:www.repository.utl.pt:10400.5/13872Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:59:47.275422Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Morphological, biochemical and molecular approaches to the identification of Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenaria in Portugal
title Morphological, biochemical and molecular approaches to the identification of Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenaria in Portugal
spellingShingle Morphological, biochemical and molecular approaches to the identification of Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenaria in Portugal
Rusinque, Leidy Constanza Mora
root-knot nematode
Meloidogyne spp.
perineal patterns
esterase phenotype
PCR
title_short Morphological, biochemical and molecular approaches to the identification of Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenaria in Portugal
title_full Morphological, biochemical and molecular approaches to the identification of Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenaria in Portugal
title_fullStr Morphological, biochemical and molecular approaches to the identification of Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenaria in Portugal
title_full_unstemmed Morphological, biochemical and molecular approaches to the identification of Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenaria in Portugal
title_sort Morphological, biochemical and molecular approaches to the identification of Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenaria in Portugal
author Rusinque, Leidy Constanza Mora
author_facet Rusinque, Leidy Constanza Mora
author_role author
dc.contributor.none.fl_str_mv Nóbrega, Maria Filomena de Sousa
Mota, Mariana Gomes
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Rusinque, Leidy Constanza Mora
dc.subject.por.fl_str_mv root-knot nematode
Meloidogyne spp.
perineal patterns
esterase phenotype
PCR
topic root-knot nematode
Meloidogyne spp.
perineal patterns
esterase phenotype
PCR
description Mestrado em Engenharia Agonómica - Proteção de plantas - Instituto Superior de Agronomia - UL
publishDate 2017
dc.date.none.fl_str_mv 2017-07-07T09:46:40Z
2017
2017-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/13872
url http://hdl.handle.net/10400.5/13872
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Rusinque, L.C.M. - Morphological, biochemical and molecular approaches to the identification of Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenaria in Portugal. Lisboa: ISA, 2017, 80 p., 4 apend.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ISA/UL
publisher.none.fl_str_mv ISA/UL
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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