The quantitative architecture of centromeric chromatin
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/492 |
Resumo: | The centromere, responsible for chromosome segregation during mitosis, is epigenetically defined by CENP-A containing chromatin. The amount of centromeric CENP-A has direct implications for both the architecture and epigenetic inheritance of centromeres. Using complementary strategies, we determined that typical human centromeres contain ∼400 molecules of CENP-A, which is controlled by a mass-action mechanism. This number, despite representing only ∼4% of all centromeric nucleosomes, forms a ∼50-fold enrichment to the overall genome. In addition, although pre-assembled CENP-A is randomly segregated during cell division, this amount of CENP-A is sufficient to prevent stochastic loss of centromere function and identity. Finally, we produced a statistical map of CENP-A occupancy at a human neocentromere and identified nucleosome positions that feature CENP-A in a majority of cells. In summary, we present a quantitative view of the centromere that provides a mechanistic framework for both robust epigenetic inheritance of centromeres and the paucity of neocentromere formation.DOI: http://dx.doi.org/10.7554/eLife.02137.001. |
id |
RCAP_217754dc73fc33c2a68739015c122b88 |
---|---|
oai_identifier_str |
oai:arca.igc.gulbenkian.pt:10400.7/492 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
The quantitative architecture of centromeric chromatinCENP-Acentromereepigeneticshistone variantmolecular countingquantitative microscopyThe centromere, responsible for chromosome segregation during mitosis, is epigenetically defined by CENP-A containing chromatin. The amount of centromeric CENP-A has direct implications for both the architecture and epigenetic inheritance of centromeres. Using complementary strategies, we determined that typical human centromeres contain ∼400 molecules of CENP-A, which is controlled by a mass-action mechanism. This number, despite representing only ∼4% of all centromeric nucleosomes, forms a ∼50-fold enrichment to the overall genome. In addition, although pre-assembled CENP-A is randomly segregated during cell division, this amount of CENP-A is sufficient to prevent stochastic loss of centromere function and identity. Finally, we produced a statistical map of CENP-A occupancy at a human neocentromere and identified nucleosome positions that feature CENP-A in a majority of cells. In summary, we present a quantitative view of the centromere that provides a mechanistic framework for both robust epigenetic inheritance of centromeres and the paucity of neocentromere formation.DOI: http://dx.doi.org/10.7554/eLife.02137.001.European Molecular Biology Organization: (EMBO Installation grant), European Commission: (FP7 Marie Curie Reintegration grant), National Institutes of Health grants: (GM082989, GM077238), Burroughs Wellcome Fund, Rita Allen Foundation, Beckman Laser Institute and Foundation, FCT fellowship: (SFRH/BD/74284/2010).Elife Sciences PublicationsARCABodor, Dani LMata, João FSergeev, MikhailDavid, Ana FilipaSalimian, Kevan JPanchenko, TanyaCleveland, Don WBlack, Ben EShah, Jagesh VJansen, Lars ET2015-11-12T16:30:38Z2014-07-152014-07-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/492eng10.7554/eLife.02137info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:34:52Zoai:arca.igc.gulbenkian.pt:10400.7/492Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:45.938611Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The quantitative architecture of centromeric chromatin |
title |
The quantitative architecture of centromeric chromatin |
spellingShingle |
The quantitative architecture of centromeric chromatin Bodor, Dani L CENP-A centromere epigenetics histone variant molecular counting quantitative microscopy |
title_short |
The quantitative architecture of centromeric chromatin |
title_full |
The quantitative architecture of centromeric chromatin |
title_fullStr |
The quantitative architecture of centromeric chromatin |
title_full_unstemmed |
The quantitative architecture of centromeric chromatin |
title_sort |
The quantitative architecture of centromeric chromatin |
author |
Bodor, Dani L |
author_facet |
Bodor, Dani L Mata, João F Sergeev, Mikhail David, Ana Filipa Salimian, Kevan J Panchenko, Tanya Cleveland, Don W Black, Ben E Shah, Jagesh V Jansen, Lars ET |
author_role |
author |
author2 |
Mata, João F Sergeev, Mikhail David, Ana Filipa Salimian, Kevan J Panchenko, Tanya Cleveland, Don W Black, Ben E Shah, Jagesh V Jansen, Lars ET |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Bodor, Dani L Mata, João F Sergeev, Mikhail David, Ana Filipa Salimian, Kevan J Panchenko, Tanya Cleveland, Don W Black, Ben E Shah, Jagesh V Jansen, Lars ET |
dc.subject.por.fl_str_mv |
CENP-A centromere epigenetics histone variant molecular counting quantitative microscopy |
topic |
CENP-A centromere epigenetics histone variant molecular counting quantitative microscopy |
description |
The centromere, responsible for chromosome segregation during mitosis, is epigenetically defined by CENP-A containing chromatin. The amount of centromeric CENP-A has direct implications for both the architecture and epigenetic inheritance of centromeres. Using complementary strategies, we determined that typical human centromeres contain ∼400 molecules of CENP-A, which is controlled by a mass-action mechanism. This number, despite representing only ∼4% of all centromeric nucleosomes, forms a ∼50-fold enrichment to the overall genome. In addition, although pre-assembled CENP-A is randomly segregated during cell division, this amount of CENP-A is sufficient to prevent stochastic loss of centromere function and identity. Finally, we produced a statistical map of CENP-A occupancy at a human neocentromere and identified nucleosome positions that feature CENP-A in a majority of cells. In summary, we present a quantitative view of the centromere that provides a mechanistic framework for both robust epigenetic inheritance of centromeres and the paucity of neocentromere formation.DOI: http://dx.doi.org/10.7554/eLife.02137.001. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-07-15 2014-07-15T00:00:00Z 2015-11-12T16:30:38Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/492 |
url |
http://hdl.handle.net/10400.7/492 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.7554/eLife.02137 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elife Sciences Publications |
publisher.none.fl_str_mv |
Elife Sciences Publications |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799130572981796864 |