Healing activity evaluation of the galactomannan film obtained from Cassia grandis seeds with immobilized Cratylia mollis seed lectin
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/47688 |
Resumo: | Galactomannan films from Cassia grandis seeds, associated or not with Cramoll 1,4, were used on topical wounds of rats for the evaluation of the healing process during 14 days. All of the films were evaluated by cytotoxic assay, FTIR and lectin hemagglutinating activity (HA). Forty-five male rats were submitted to aseptic dermal wounds (Ø = 0.8 cm) and divided in groups (n = 15): control, test 1, and test 2, treated respectively with saline, galactomannan film and film with immobilized Cramoll 1,4. Macroscopic evaluations were performed by clinical observations and area measurements, and microscopic analysis by histological criteria. Epithelial cell proliferation and differentiation was immunohistochemically assessed using CK14 and PCNA. The presence of CO peaks in the FTIR spectrum confirmed the immobilization of Cramoll 1,4 in the film, while the residual HA confirmed the stability of the lectin after immobilization with 90.94% of the initial HA. The films presented non-cytotoxicity and cell viability exceeding 80%. All of the animals presented re-epithelization around 10 days, furthermore test 2 group showed a diffuse response at the stromal tissue and the basal layer associated with wounds completely closed with 11 days of experiment. The results suggest a promising use of the films as topical wound curatives. |
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Healing activity evaluation of the galactomannan film obtained from Cassia grandis seeds with immobilized Cratylia mollis seed lectinNatural polymersTopical wound curativesWound healingScience & TechnologyGalactomannan films from Cassia grandis seeds, associated or not with Cramoll 1,4, were used on topical wounds of rats for the evaluation of the healing process during 14 days. All of the films were evaluated by cytotoxic assay, FTIR and lectin hemagglutinating activity (HA). Forty-five male rats were submitted to aseptic dermal wounds (Ø = 0.8 cm) and divided in groups (n = 15): control, test 1, and test 2, treated respectively with saline, galactomannan film and film with immobilized Cramoll 1,4. Macroscopic evaluations were performed by clinical observations and area measurements, and microscopic analysis by histological criteria. Epithelial cell proliferation and differentiation was immunohistochemically assessed using CK14 and PCNA. The presence of CO peaks in the FTIR spectrum confirmed the immobilization of Cramoll 1,4 in the film, while the residual HA confirmed the stability of the lectin after immobilization with 90.94% of the initial HA. The films presented non-cytotoxicity and cell viability exceeding 80%. All of the animals presented re-epithelization around 10 days, furthermore test 2 group showed a diffuse response at the stromal tissue and the basal layer associated with wounds completely closed with 11 days of experiment. The results suggest a promising use of the films as topical wound curatives.PBSA and ACAN are recipients of a scholarship from the Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE), and PAGS and GSA are recipients from the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES). The authors express their gratitude to the Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq) for research grants and fellowships (MTSC, LCBBC and MGCC).info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoAlbuquerque, Priscilla B. S.Soares, Paulo A. G.Aragão-Neto, Adelmo C.Albuquerque, Giwellington S.Silva, Luís C. N.Lima-Ribeiro, Maria H. M.Neto, Jacinto C. SilvaCoelho, Luana C. B. B.Correia, Maria T. S.Teixeira, J. A.Carneiro-da-Cunha, Maria G.2017-092017-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/47688engAlbuquerque, Priscilla B. S.; Soares, Paulo A. G.; Aragão-Neto, Adelmo C.; Albuquerque, Giwellington S.; Silva, Luís C. N.; Lima-Ribeiro, Maria H. M.; Neto, Jacinto C. Silva; Coelho, Luana C. B. B.; Correia, Maria T. S.; Teixeira, José A.; Carneiro-da-Cunha, Maria G., Healing activity evaluation of the galactomannan film obtained from Cassia grandis seeds with immobilized Cratylia mollis seed lectin. International Journal of Biological Macromolecules, 102, 749-757, 20170141-813010.1016/j.ijbiomac.2017.04.06428433769http://www.elsevier.com/locate/issn/01418130info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:40:22ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Healing activity evaluation of the galactomannan film obtained from Cassia grandis seeds with immobilized Cratylia mollis seed lectin |
title |
Healing activity evaluation of the galactomannan film obtained from Cassia grandis seeds with immobilized Cratylia mollis seed lectin |
spellingShingle |
Healing activity evaluation of the galactomannan film obtained from Cassia grandis seeds with immobilized Cratylia mollis seed lectin Albuquerque, Priscilla B. S. Natural polymers Topical wound curatives Wound healing Science & Technology |
title_short |
Healing activity evaluation of the galactomannan film obtained from Cassia grandis seeds with immobilized Cratylia mollis seed lectin |
title_full |
Healing activity evaluation of the galactomannan film obtained from Cassia grandis seeds with immobilized Cratylia mollis seed lectin |
title_fullStr |
Healing activity evaluation of the galactomannan film obtained from Cassia grandis seeds with immobilized Cratylia mollis seed lectin |
title_full_unstemmed |
Healing activity evaluation of the galactomannan film obtained from Cassia grandis seeds with immobilized Cratylia mollis seed lectin |
title_sort |
Healing activity evaluation of the galactomannan film obtained from Cassia grandis seeds with immobilized Cratylia mollis seed lectin |
author |
Albuquerque, Priscilla B. S. |
author_facet |
Albuquerque, Priscilla B. S. Soares, Paulo A. G. Aragão-Neto, Adelmo C. Albuquerque, Giwellington S. Silva, Luís C. N. Lima-Ribeiro, Maria H. M. Neto, Jacinto C. Silva Coelho, Luana C. B. B. Correia, Maria T. S. Teixeira, J. A. Carneiro-da-Cunha, Maria G. |
author_role |
author |
author2 |
Soares, Paulo A. G. Aragão-Neto, Adelmo C. Albuquerque, Giwellington S. Silva, Luís C. N. Lima-Ribeiro, Maria H. M. Neto, Jacinto C. Silva Coelho, Luana C. B. B. Correia, Maria T. S. Teixeira, J. A. Carneiro-da-Cunha, Maria G. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Albuquerque, Priscilla B. S. Soares, Paulo A. G. Aragão-Neto, Adelmo C. Albuquerque, Giwellington S. Silva, Luís C. N. Lima-Ribeiro, Maria H. M. Neto, Jacinto C. Silva Coelho, Luana C. B. B. Correia, Maria T. S. Teixeira, J. A. Carneiro-da-Cunha, Maria G. |
dc.subject.por.fl_str_mv |
Natural polymers Topical wound curatives Wound healing Science & Technology |
topic |
Natural polymers Topical wound curatives Wound healing Science & Technology |
description |
Galactomannan films from Cassia grandis seeds, associated or not with Cramoll 1,4, were used on topical wounds of rats for the evaluation of the healing process during 14 days. All of the films were evaluated by cytotoxic assay, FTIR and lectin hemagglutinating activity (HA). Forty-five male rats were submitted to aseptic dermal wounds (Ø = 0.8 cm) and divided in groups (n = 15): control, test 1, and test 2, treated respectively with saline, galactomannan film and film with immobilized Cramoll 1,4. Macroscopic evaluations were performed by clinical observations and area measurements, and microscopic analysis by histological criteria. Epithelial cell proliferation and differentiation was immunohistochemically assessed using CK14 and PCNA. The presence of CO peaks in the FTIR spectrum confirmed the immobilization of Cramoll 1,4 in the film, while the residual HA confirmed the stability of the lectin after immobilization with 90.94% of the initial HA. The films presented non-cytotoxicity and cell viability exceeding 80%. All of the animals presented re-epithelization around 10 days, furthermore test 2 group showed a diffuse response at the stromal tissue and the basal layer associated with wounds completely closed with 11 days of experiment. The results suggest a promising use of the films as topical wound curatives. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09 2017-09-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/47688 |
url |
http://hdl.handle.net/1822/47688 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Albuquerque, Priscilla B. S.; Soares, Paulo A. G.; Aragão-Neto, Adelmo C.; Albuquerque, Giwellington S.; Silva, Luís C. N.; Lima-Ribeiro, Maria H. M.; Neto, Jacinto C. Silva; Coelho, Luana C. B. B.; Correia, Maria T. S.; Teixeira, José A.; Carneiro-da-Cunha, Maria G., Healing activity evaluation of the galactomannan film obtained from Cassia grandis seeds with immobilized Cratylia mollis seed lectin. International Journal of Biological Macromolecules, 102, 749-757, 2017 0141-8130 10.1016/j.ijbiomac.2017.04.064 28433769 http://www.elsevier.com/locate/issn/01418130 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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