Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiae
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/69633 |
Resumo: | "Available online 07 January 2021" |
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Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiaeLactoferrinYeast cell deathLipid raftsPma1V-ATPasePma1pScience & Technology"Available online 07 January 2021"Lactoferrin (Lf) is a bioactive milk-derived protein with remarkable wide-spectrum antifungal activity. To deepen our understanding of the molecular mechanisms underlying Lf cytotoxicity, the role of plasma membrane ergosterol- and sphingolipid-rich lipid rafts and their association with the proton pump Pma1p was explored. Pma1p was previously identified as a Lf-binding protein. Results showed that bovine Lf (bLf) perturbs sterol-rich lipid rafts organization by inducing intracellular accumulation of ergosterol. Using yeast mutant strains lacking lipid rafts-associated proteins or enzymes involved in the synthesis of ergosterol and sphingolipids, we found that perturbations in the composition of these membrane domains increase resistance to bLf-induced yeast cell death. Also, when Pma1p-lipid rafts association is compromised in the Pma110 mutant and in the absence of the Pma1p-binding protein Ast1p, the bLf killing activity is impaired. Altogether, results showed that the perturbation of lipid rafts and the inhibition of both Pma1p and V-ATPase activities mediate the antifungal activity of bLf. Since it is suggested that the combination of conventional antifungals with lipid rafts-disrupting compounds is a powerful antifungal approach, our data will help to pave the way for the use of bLf alone or in combination for the treatment/eradication of clinically and agronomically relevant yeast pathogens/fungi.This work was supported by national funds through the Portuguese Foundation for Science and Technology (FCT I.P.) under the scope of the strategic funding of “Contrato-Programa” UIDB/04050/2020 and UIDB/ 04469/2020 unit; by the BioTecNorte operation (NORTE-01-0145- FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte; and by the Servicio para el Control de la Esterilización, Laboratorio de Microbiología Oral (CN-16-036). Cátia Santos-Pereira acknowledges the PhD fellowship PD/BD/128032/2016 funded by FCT under the scope of the doctoral programme in Applied and Environmental Microbiology (DP_AEM).info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoPereira, Cátia Sofia SantosAndrés, María T.Chaves, Susana Alexandra RodriguesFierro, José F.Gerós, H.Manon, StéphenRodrigues, L. R.Côrte-Real, Manuela2021-02-282021-02-28T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/69633engCátia S. Pereira; Andrés, María T.; Chaves, Susana R.; Fierro, José F.; Gerós, Hernâni; Manon, Stéphen; Rodrigues, Lígia R.; Côrte-Real, Manuela, Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiae. International Journal of Biological Macromolecules, 171, 343-357, 20210141-813010.1016/j.ijbiomac.2020.12.22433421469http://www.elsevier.com/locate/issn/01418130info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:10:36Zoai:repositorium.sdum.uminho.pt:1822/69633Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:02:15.485346Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiae |
| title |
Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiae |
| spellingShingle |
Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiae Pereira, Cátia Sofia Santos Lactoferrin Yeast cell death Lipid rafts Pma1 V-ATPase Pma1p Science & Technology |
| title_short |
Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiae |
| title_full |
Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiae |
| title_fullStr |
Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiae |
| title_full_unstemmed |
Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiae |
| title_sort |
Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiae |
| author |
Pereira, Cátia Sofia Santos |
| author_facet |
Pereira, Cátia Sofia Santos Andrés, María T. Chaves, Susana Alexandra Rodrigues Fierro, José F. Gerós, H. Manon, Stéphen Rodrigues, L. R. Côrte-Real, Manuela |
| author_role |
author |
| author2 |
Andrés, María T. Chaves, Susana Alexandra Rodrigues Fierro, José F. Gerós, H. Manon, Stéphen Rodrigues, L. R. Côrte-Real, Manuela |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Pereira, Cátia Sofia Santos Andrés, María T. Chaves, Susana Alexandra Rodrigues Fierro, José F. Gerós, H. Manon, Stéphen Rodrigues, L. R. Côrte-Real, Manuela |
| dc.subject.por.fl_str_mv |
Lactoferrin Yeast cell death Lipid rafts Pma1 V-ATPase Pma1p Science & Technology |
| topic |
Lactoferrin Yeast cell death Lipid rafts Pma1 V-ATPase Pma1p Science & Technology |
| description |
"Available online 07 January 2021" |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-02-28 2021-02-28T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/69633 |
| url |
http://hdl.handle.net/1822/69633 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Cátia S. Pereira; Andrés, María T.; Chaves, Susana R.; Fierro, José F.; Gerós, Hernâni; Manon, Stéphen; Rodrigues, Lígia R.; Côrte-Real, Manuela, Lactoferrin perturbs lipid rafts and requires integrity of Pma1p-lipid rafts association to exert its antifungal activity against Saccharomyces cerevisiae. International Journal of Biological Macromolecules, 171, 343-357, 2021 0141-8130 10.1016/j.ijbiomac.2020.12.224 33421469 http://www.elsevier.com/locate/issn/01418130 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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