Proteome analysis of vitreous humor in retinal detachment using two different flow-charts for protein fractionation

Detalhes bibliográficos
Autor(a) principal: Gaspar, Leonor M.
Data de Publicação: 2017
Outros Autores: Santos, F.M., Albuquerque, Tânia, Sousa, João P. Castro de, Passarinha, L A, Tomaz, Cândida T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.6/9143
Resumo: The deeper understanding of retinal detachment (RD) pathogenesis may improve the visual outcome after surgery. Given the main role of the vitreous in retinal eye diseases, two strategies were explored to identify its proteome in RD. Fractionation techniques such as anion exchange chromatography (IEX) and SDS-PAGE combined with MALDI-TOF/TOF analysis allowed to identify 127 proteins in vitreous of RD patients. From these proteins, 19 were identified using only the IEX fractionation strategy, and 117 using a bidimensional (IEX and SDS-PAGE) fractionation. Of these proteins, 68 had not yet been found in other vitreous proteomic studies. The fractionation with IEX and SDS-PAGE largely improved the number of identified proteins proving that it is crucial to combine several methodologies to cover vitreous proteome.
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spelling Proteome analysis of vitreous humor in retinal detachment using two different flow-charts for protein fractionationProteomic AnalysisMass spectrometryRetinal DetachmentVitreous HumourElectrophoresisLIquid ChromatographyThe deeper understanding of retinal detachment (RD) pathogenesis may improve the visual outcome after surgery. Given the main role of the vitreous in retinal eye diseases, two strategies were explored to identify its proteome in RD. Fractionation techniques such as anion exchange chromatography (IEX) and SDS-PAGE combined with MALDI-TOF/TOF analysis allowed to identify 127 proteins in vitreous of RD patients. From these proteins, 19 were identified using only the IEX fractionation strategy, and 117 using a bidimensional (IEX and SDS-PAGE) fractionation. Of these proteins, 68 had not yet been found in other vitreous proteomic studies. The fractionation with IEX and SDS-PAGE largely improved the number of identified proteins proving that it is crucial to combine several methodologies to cover vitreous proteome.CENTRO-07-ST24-FEDER-002014;Novartis Farma-Produtos Farmacêuticos, SA; Project POCI-01-0145-FEDER-007491ElsevieruBibliorumGaspar, Leonor M.Santos, F.M.Albuquerque, TâniaSousa, João P. Castro dePassarinha, L ATomaz, Cândida T.2022-07-29T00:30:18Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/9143eng10.1016/j.jchromb.2017.07.049info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-01-16T11:54:51ZPortal AgregadorONG
dc.title.none.fl_str_mv Proteome analysis of vitreous humor in retinal detachment using two different flow-charts for protein fractionation
title Proteome analysis of vitreous humor in retinal detachment using two different flow-charts for protein fractionation
spellingShingle Proteome analysis of vitreous humor in retinal detachment using two different flow-charts for protein fractionation
Gaspar, Leonor M.
Proteomic Analysis
Mass spectrometry
Retinal Detachment
Vitreous Humour
Electrophoresis
LIquid Chromatography
title_short Proteome analysis of vitreous humor in retinal detachment using two different flow-charts for protein fractionation
title_full Proteome analysis of vitreous humor in retinal detachment using two different flow-charts for protein fractionation
title_fullStr Proteome analysis of vitreous humor in retinal detachment using two different flow-charts for protein fractionation
title_full_unstemmed Proteome analysis of vitreous humor in retinal detachment using two different flow-charts for protein fractionation
title_sort Proteome analysis of vitreous humor in retinal detachment using two different flow-charts for protein fractionation
author Gaspar, Leonor M.
author_facet Gaspar, Leonor M.
Santos, F.M.
Albuquerque, Tânia
Sousa, João P. Castro de
Passarinha, L A
Tomaz, Cândida T.
author_role author
author2 Santos, F.M.
Albuquerque, Tânia
Sousa, João P. Castro de
Passarinha, L A
Tomaz, Cândida T.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv uBibliorum
dc.contributor.author.fl_str_mv Gaspar, Leonor M.
Santos, F.M.
Albuquerque, Tânia
Sousa, João P. Castro de
Passarinha, L A
Tomaz, Cândida T.
dc.subject.por.fl_str_mv Proteomic Analysis
Mass spectrometry
Retinal Detachment
Vitreous Humour
Electrophoresis
LIquid Chromatography
topic Proteomic Analysis
Mass spectrometry
Retinal Detachment
Vitreous Humour
Electrophoresis
LIquid Chromatography
description The deeper understanding of retinal detachment (RD) pathogenesis may improve the visual outcome after surgery. Given the main role of the vitreous in retinal eye diseases, two strategies were explored to identify its proteome in RD. Fractionation techniques such as anion exchange chromatography (IEX) and SDS-PAGE combined with MALDI-TOF/TOF analysis allowed to identify 127 proteins in vitreous of RD patients. From these proteins, 19 were identified using only the IEX fractionation strategy, and 117 using a bidimensional (IEX and SDS-PAGE) fractionation. Of these proteins, 68 had not yet been found in other vitreous proteomic studies. The fractionation with IEX and SDS-PAGE largely improved the number of identified proteins proving that it is crucial to combine several methodologies to cover vitreous proteome.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2022-07-29T00:30:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.6/9143
url http://hdl.handle.net/10400.6/9143
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.jchromb.2017.07.049
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.mail.fl_str_mv
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