Biotribocorrosion (tribo-electrochemical) characterization of anodized titanium biomaterial containing calcium and phosphorus before and after osteoblastic cell culture
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/35319 |
Resumo: | The purpose of this study was to investigate the relationship between the osteoblastic cells behavior and biotribocorrosion phenomena on bioactive titanium (Ti). Ti substrates submitted to bioactive anodic oxidation and etching treatments were cultured up to 28 days with MG63 osteoblast-like cells. Important parameters of in vitro bone-like tissue formation were assessed. Although no major differences were observed between the surfaces topography (both rough) and wettability (both hydrophobic), a significant increase in cell attachment and differentiation was detected on the anodized substrates as product of favorable surface morphology and chemical composition. Alkaline phosphatase production has increased (20 nmol/min/mg of protein) on the anodized materials, while phosphate concentration has reached the double of the etched material and calcium production increased (over 20 µg/mL). The mechanical and biological stability of the anodic surfaces were also put to test through biotribocorrosion sliding solicitations, putting in evidence the resistance of the anodic layer and the cells capacity of regeneration after implant degradation. The Ti osteointegration abilities were also confirmed by the development of strong cell-biomaterial bonds at the interface, on both substrates. By combining the biological and mechanical results, the anodized Ti can be considered a viable option for dentistry. |
id |
RCAP_24e1cdd4123caecd62ede5341c3b44c8 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/35319 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
|
spelling |
Biotribocorrosion (tribo-electrochemical) characterization of anodized titanium biomaterial containing calcium and phosphorus before and after osteoblastic cell culturebiotribocorrosiontitanium biomaterialanodizationosteoblastic cellswearingEngenharia e Tecnologia::Biotecnologia IndustrialCiências Médicas::Biotecnologia MédicaScience & TechnologyThe purpose of this study was to investigate the relationship between the osteoblastic cells behavior and biotribocorrosion phenomena on bioactive titanium (Ti). Ti substrates submitted to bioactive anodic oxidation and etching treatments were cultured up to 28 days with MG63 osteoblast-like cells. Important parameters of in vitro bone-like tissue formation were assessed. Although no major differences were observed between the surfaces topography (both rough) and wettability (both hydrophobic), a significant increase in cell attachment and differentiation was detected on the anodized substrates as product of favorable surface morphology and chemical composition. Alkaline phosphatase production has increased (20 nmol/min/mg of protein) on the anodized materials, while phosphate concentration has reached the double of the etched material and calcium production increased (over 20 µg/mL). The mechanical and biological stability of the anodic surfaces were also put to test through biotribocorrosion sliding solicitations, putting in evidence the resistance of the anodic layer and the cells capacity of regeneration after implant degradation. The Ti osteointegration abilities were also confirmed by the development of strong cell-biomaterial bonds at the interface, on both substrates. By combining the biological and mechanical results, the anodized Ti can be considered a viable option for dentistry.John Wiley and SonsUniversidade do MinhoFelgueiras, Helena PradoCastanheira, L.Changotade, S.Poirier, F.Oughlis, S.Henriques, MarianaChakar, C.Naaman, N.Younes, R.Migonney, V.Celis, J. P.Ponthiaux, PierreRocha, L. A.Lutomski, D.20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/35319engFelgueiras, H. P.; Castanheira, L.; Changotade, S.; Poirier, F.; Oughlis, S.; Henriques, Mariana; Chakar, C.; Naaman, N.; Younes, R.; Migonney, V.; Celis, J. P.; Ponthiaux, P.; Rocha, L. A.; Lutomski, D., Biotribocorrosion (tribo-electrochemical) characterization of anodized titanium biomaterial containing calcium and phosphorus before and after osteoblastic cell culture. Journal of Biomedical Materials Research - Part B: Applied Biomaterials, 103(3), 661-669, 2015.1552-49731552-498110.1002/jbm.b.3323624989830http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33236/abstractinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:03:53ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Biotribocorrosion (tribo-electrochemical) characterization of anodized titanium biomaterial containing calcium and phosphorus before and after osteoblastic cell culture |
title |
Biotribocorrosion (tribo-electrochemical) characterization of anodized titanium biomaterial containing calcium and phosphorus before and after osteoblastic cell culture |
spellingShingle |
Biotribocorrosion (tribo-electrochemical) characterization of anodized titanium biomaterial containing calcium and phosphorus before and after osteoblastic cell culture Felgueiras, Helena Prado biotribocorrosion titanium biomaterial anodization osteoblastic cells wearing Engenharia e Tecnologia::Biotecnologia Industrial Ciências Médicas::Biotecnologia Médica Science & Technology |
title_short |
Biotribocorrosion (tribo-electrochemical) characterization of anodized titanium biomaterial containing calcium and phosphorus before and after osteoblastic cell culture |
title_full |
Biotribocorrosion (tribo-electrochemical) characterization of anodized titanium biomaterial containing calcium and phosphorus before and after osteoblastic cell culture |
title_fullStr |
Biotribocorrosion (tribo-electrochemical) characterization of anodized titanium biomaterial containing calcium and phosphorus before and after osteoblastic cell culture |
title_full_unstemmed |
Biotribocorrosion (tribo-electrochemical) characterization of anodized titanium biomaterial containing calcium and phosphorus before and after osteoblastic cell culture |
title_sort |
Biotribocorrosion (tribo-electrochemical) characterization of anodized titanium biomaterial containing calcium and phosphorus before and after osteoblastic cell culture |
author |
Felgueiras, Helena Prado |
author_facet |
Felgueiras, Helena Prado Castanheira, L. Changotade, S. Poirier, F. Oughlis, S. Henriques, Mariana Chakar, C. Naaman, N. Younes, R. Migonney, V. Celis, J. P. Ponthiaux, Pierre Rocha, L. A. Lutomski, D. |
author_role |
author |
author2 |
Castanheira, L. Changotade, S. Poirier, F. Oughlis, S. Henriques, Mariana Chakar, C. Naaman, N. Younes, R. Migonney, V. Celis, J. P. Ponthiaux, Pierre Rocha, L. A. Lutomski, D. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Felgueiras, Helena Prado Castanheira, L. Changotade, S. Poirier, F. Oughlis, S. Henriques, Mariana Chakar, C. Naaman, N. Younes, R. Migonney, V. Celis, J. P. Ponthiaux, Pierre Rocha, L. A. Lutomski, D. |
dc.subject.por.fl_str_mv |
biotribocorrosion titanium biomaterial anodization osteoblastic cells wearing Engenharia e Tecnologia::Biotecnologia Industrial Ciências Médicas::Biotecnologia Médica Science & Technology |
topic |
biotribocorrosion titanium biomaterial anodization osteoblastic cells wearing Engenharia e Tecnologia::Biotecnologia Industrial Ciências Médicas::Biotecnologia Médica Science & Technology |
description |
The purpose of this study was to investigate the relationship between the osteoblastic cells behavior and biotribocorrosion phenomena on bioactive titanium (Ti). Ti substrates submitted to bioactive anodic oxidation and etching treatments were cultured up to 28 days with MG63 osteoblast-like cells. Important parameters of in vitro bone-like tissue formation were assessed. Although no major differences were observed between the surfaces topography (both rough) and wettability (both hydrophobic), a significant increase in cell attachment and differentiation was detected on the anodized substrates as product of favorable surface morphology and chemical composition. Alkaline phosphatase production has increased (20 nmol/min/mg of protein) on the anodized materials, while phosphate concentration has reached the double of the etched material and calcium production increased (over 20 µg/mL). The mechanical and biological stability of the anodic surfaces were also put to test through biotribocorrosion sliding solicitations, putting in evidence the resistance of the anodic layer and the cells capacity of regeneration after implant degradation. The Ti osteointegration abilities were also confirmed by the development of strong cell-biomaterial bonds at the interface, on both substrates. By combining the biological and mechanical results, the anodized Ti can be considered a viable option for dentistry. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015 2015-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/35319 |
url |
https://hdl.handle.net/1822/35319 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Felgueiras, H. P.; Castanheira, L.; Changotade, S.; Poirier, F.; Oughlis, S.; Henriques, Mariana; Chakar, C.; Naaman, N.; Younes, R.; Migonney, V.; Celis, J. P.; Ponthiaux, P.; Rocha, L. A.; Lutomski, D., Biotribocorrosion (tribo-electrochemical) characterization of anodized titanium biomaterial containing calcium and phosphorus before and after osteoblastic cell culture. Journal of Biomedical Materials Research - Part B: Applied Biomaterials, 103(3), 661-669, 2015. 1552-4973 1552-4981 10.1002/jbm.b.33236 24989830 http://onlinelibrary.wiley.com/doi/10.1002/jbm.b.33236/abstract |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
John Wiley and Sons |
publisher.none.fl_str_mv |
John Wiley and Sons |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
_version_ |
1777303673806782464 |