Activation-Induced Cytidine Deaminase Does Not Impact Murine Meiotic Recombination
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/443 |
Resumo: | Activation-Induced Cytidine Deaminase (AID) was first described as the triggering enzyme of the B-cell-specific reactions that edit the immunoglobulin genes, namely somatic hypermutation, gene conversion and class switch recombination. Over the years, AID was also detected in cells other than lymphocytes, and it has been assigned additional roles in the innate defense against transforming retroviruses, in retrotransposition restriction and in DNA demethylation. Notably, AID expression was found in germline tissues, and in heterologous systems it can induce the double-strand breaks required for the initiation of meiotic recombination and proper gamete formation. However, since AID deficient mice are fully fertile, the molecule is not essential for meiosis. Thus, the remaining question that we addressed here is whether AID influences the frequency of meiotic recombination in mice. We measured the recombination events in the meiosis of male and female mice F1 hybrids of C57BL/6J and BALB/c, in Aicda(+/+) and Aicda(-/-) background using a panel of SNPs that distinguishes C57BL/6J from BALB/c genome across the 19 autosomes. In agreement with the literature, we found that the frequency of recombination in the female germline was higher than in male germline, both in the Aicda(+/+) and the Aicda(-/-) backgrounds. No statistical difference was found in the average recombination events between Aicda(+/+) and Aicda(-/-) animals, either in females or males. In addition, the recombination frequency between SNPs flanking the IgH and Igκ loci was also not different. We conclude that AID has a minor impact, if any, on the overall frequency of meiotic recombination. |
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Activation-Induced Cytidine Deaminase Does Not Impact Murine Meiotic RecombinationAIDmeiotic recombinationgermlinecytidine deaminasedouble-strand breaksActivation-Induced Cytidine Deaminase (AID) was first described as the triggering enzyme of the B-cell-specific reactions that edit the immunoglobulin genes, namely somatic hypermutation, gene conversion and class switch recombination. Over the years, AID was also detected in cells other than lymphocytes, and it has been assigned additional roles in the innate defense against transforming retroviruses, in retrotransposition restriction and in DNA demethylation. Notably, AID expression was found in germline tissues, and in heterologous systems it can induce the double-strand breaks required for the initiation of meiotic recombination and proper gamete formation. However, since AID deficient mice are fully fertile, the molecule is not essential for meiosis. Thus, the remaining question that we addressed here is whether AID influences the frequency of meiotic recombination in mice. We measured the recombination events in the meiosis of male and female mice F1 hybrids of C57BL/6J and BALB/c, in Aicda(+/+) and Aicda(-/-) background using a panel of SNPs that distinguishes C57BL/6J from BALB/c genome across the 19 autosomes. In agreement with the literature, we found that the frequency of recombination in the female germline was higher than in male germline, both in the Aicda(+/+) and the Aicda(-/-) backgrounds. No statistical difference was found in the average recombination events between Aicda(+/+) and Aicda(-/-) animals, either in females or males. In addition, the recombination frequency between SNPs flanking the IgH and Igκ loci was also not different. We conclude that AID has a minor impact, if any, on the overall frequency of meiotic recombination.Marie Curie Reintegration grant: (PIRG03-GA-2008230967), FCT fellowship: (SFRH/BPD/66824/2009).Genetics Society of AmericaARCACortesao, C. S.Freitas, R. F.Barreto, V. M.2015-10-26T16:15:00Z2013-03-112013-03-11T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/443engActivation-Induced Cytidine Deaminase Does Not Impact Murine Meiotic Recombination Catarina S. Cortesao, Raquel F. Freitas, and Vasco M. Barreto G3 April 2013 3:645-655; Early Online March 11, 2013, doi:10.1534/g3.113.00555310.1534/g3.113.005553info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:34:50Zoai:arca.igc.gulbenkian.pt:10400.7/443Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:43.882461Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Activation-Induced Cytidine Deaminase Does Not Impact Murine Meiotic Recombination |
title |
Activation-Induced Cytidine Deaminase Does Not Impact Murine Meiotic Recombination |
spellingShingle |
Activation-Induced Cytidine Deaminase Does Not Impact Murine Meiotic Recombination Cortesao, C. S. AID meiotic recombination germline cytidine deaminase double-strand breaks |
title_short |
Activation-Induced Cytidine Deaminase Does Not Impact Murine Meiotic Recombination |
title_full |
Activation-Induced Cytidine Deaminase Does Not Impact Murine Meiotic Recombination |
title_fullStr |
Activation-Induced Cytidine Deaminase Does Not Impact Murine Meiotic Recombination |
title_full_unstemmed |
Activation-Induced Cytidine Deaminase Does Not Impact Murine Meiotic Recombination |
title_sort |
Activation-Induced Cytidine Deaminase Does Not Impact Murine Meiotic Recombination |
author |
Cortesao, C. S. |
author_facet |
Cortesao, C. S. Freitas, R. F. Barreto, V. M. |
author_role |
author |
author2 |
Freitas, R. F. Barreto, V. M. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Cortesao, C. S. Freitas, R. F. Barreto, V. M. |
dc.subject.por.fl_str_mv |
AID meiotic recombination germline cytidine deaminase double-strand breaks |
topic |
AID meiotic recombination germline cytidine deaminase double-strand breaks |
description |
Activation-Induced Cytidine Deaminase (AID) was first described as the triggering enzyme of the B-cell-specific reactions that edit the immunoglobulin genes, namely somatic hypermutation, gene conversion and class switch recombination. Over the years, AID was also detected in cells other than lymphocytes, and it has been assigned additional roles in the innate defense against transforming retroviruses, in retrotransposition restriction and in DNA demethylation. Notably, AID expression was found in germline tissues, and in heterologous systems it can induce the double-strand breaks required for the initiation of meiotic recombination and proper gamete formation. However, since AID deficient mice are fully fertile, the molecule is not essential for meiosis. Thus, the remaining question that we addressed here is whether AID influences the frequency of meiotic recombination in mice. We measured the recombination events in the meiosis of male and female mice F1 hybrids of C57BL/6J and BALB/c, in Aicda(+/+) and Aicda(-/-) background using a panel of SNPs that distinguishes C57BL/6J from BALB/c genome across the 19 autosomes. In agreement with the literature, we found that the frequency of recombination in the female germline was higher than in male germline, both in the Aicda(+/+) and the Aicda(-/-) backgrounds. No statistical difference was found in the average recombination events between Aicda(+/+) and Aicda(-/-) animals, either in females or males. In addition, the recombination frequency between SNPs flanking the IgH and Igκ loci was also not different. We conclude that AID has a minor impact, if any, on the overall frequency of meiotic recombination. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-03-11 2013-03-11T00:00:00Z 2015-10-26T16:15:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/443 |
url |
http://hdl.handle.net/10400.7/443 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Activation-Induced Cytidine Deaminase Does Not Impact Murine Meiotic Recombination Catarina S. Cortesao, Raquel F. Freitas, and Vasco M. Barreto G3 April 2013 3:645-655; Early Online March 11, 2013, doi:10.1534/g3.113.005553 10.1534/g3.113.005553 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Genetics Society of America |
publisher.none.fl_str_mv |
Genetics Society of America |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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