Effects of titanium dioxide nanoparticles in human gastric epithelial cells in vitro
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10216/114862 |
Resumo: | Manufacturing or using nanomaterials may result in exposure of workers to nanoparticles. Potential routes of exposure include skin, lung and gastrointestinal tract. The lack of health-based standards for nanomaterials combined with their increasing use in many different workplaces and products emphasize the need for a reliable temporary risk assessment tool. Therefore, the aim of this work was to explore the effects of different doses of titanium dioxide nanoparticles on human gastric epithelial cells in vitro. We analyzed proliferation by MTT assay, apoptosis by Tunel, migration by injury assay, oxidative stress by determining GSH/GSSG ratio and DNA damage by Comet assay on nanoparticle-treated AGS human gastric epithelial cell line in comparison to controls. We show and discuss the tumor-like phenotypes of nanoparticles-exposed AGS cells in vitro, as increased proliferation and decreased apoptosis. Our results demonstrate for the first time that nanoparticles induce tumor-like phenotypes in human gastric epithelial cells. |
id |
RCAP_286eb9d9bfadfc64d4ca90f63eeb9099 |
---|---|
oai_identifier_str |
oai:repositorio-aberto.up.pt:10216/114862 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Effects of titanium dioxide nanoparticles in human gastric epithelial cells in vitroApoptosisGastric epithelial cellGenotoxicityOxidative stressProliferationTiO(2) nanoparticlesManufacturing or using nanomaterials may result in exposure of workers to nanoparticles. Potential routes of exposure include skin, lung and gastrointestinal tract. The lack of health-based standards for nanomaterials combined with their increasing use in many different workplaces and products emphasize the need for a reliable temporary risk assessment tool. Therefore, the aim of this work was to explore the effects of different doses of titanium dioxide nanoparticles on human gastric epithelial cells in vitro. We analyzed proliferation by MTT assay, apoptosis by Tunel, migration by injury assay, oxidative stress by determining GSH/GSSG ratio and DNA damage by Comet assay on nanoparticle-treated AGS human gastric epithelial cell line in comparison to controls. We show and discuss the tumor-like phenotypes of nanoparticles-exposed AGS cells in vitro, as increased proliferation and decreased apoptosis. Our results demonstrate for the first time that nanoparticles induce tumor-like phenotypes in human gastric epithelial cells.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10216/114862eng0753-3322 10.1016/j.biopha.2013.08.006Botelho, MCCosta, CSilva, SCosta, SDhawan, AOliveira, PAinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T14:47:27Zoai:repositorio-aberto.up.pt:10216/114862Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:08:32.097407Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effects of titanium dioxide nanoparticles in human gastric epithelial cells in vitro |
title |
Effects of titanium dioxide nanoparticles in human gastric epithelial cells in vitro |
spellingShingle |
Effects of titanium dioxide nanoparticles in human gastric epithelial cells in vitro Botelho, MC Apoptosis Gastric epithelial cell Genotoxicity Oxidative stress Proliferation TiO(2) nanoparticles |
title_short |
Effects of titanium dioxide nanoparticles in human gastric epithelial cells in vitro |
title_full |
Effects of titanium dioxide nanoparticles in human gastric epithelial cells in vitro |
title_fullStr |
Effects of titanium dioxide nanoparticles in human gastric epithelial cells in vitro |
title_full_unstemmed |
Effects of titanium dioxide nanoparticles in human gastric epithelial cells in vitro |
title_sort |
Effects of titanium dioxide nanoparticles in human gastric epithelial cells in vitro |
author |
Botelho, MC |
author_facet |
Botelho, MC Costa, C Silva, S Costa, S Dhawan, A Oliveira, PA |
author_role |
author |
author2 |
Costa, C Silva, S Costa, S Dhawan, A Oliveira, PA |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Botelho, MC Costa, C Silva, S Costa, S Dhawan, A Oliveira, PA |
dc.subject.por.fl_str_mv |
Apoptosis Gastric epithelial cell Genotoxicity Oxidative stress Proliferation TiO(2) nanoparticles |
topic |
Apoptosis Gastric epithelial cell Genotoxicity Oxidative stress Proliferation TiO(2) nanoparticles |
description |
Manufacturing or using nanomaterials may result in exposure of workers to nanoparticles. Potential routes of exposure include skin, lung and gastrointestinal tract. The lack of health-based standards for nanomaterials combined with their increasing use in many different workplaces and products emphasize the need for a reliable temporary risk assessment tool. Therefore, the aim of this work was to explore the effects of different doses of titanium dioxide nanoparticles on human gastric epithelial cells in vitro. We analyzed proliferation by MTT assay, apoptosis by Tunel, migration by injury assay, oxidative stress by determining GSH/GSSG ratio and DNA damage by Comet assay on nanoparticle-treated AGS human gastric epithelial cell line in comparison to controls. We show and discuss the tumor-like phenotypes of nanoparticles-exposed AGS cells in vitro, as increased proliferation and decreased apoptosis. Our results demonstrate for the first time that nanoparticles induce tumor-like phenotypes in human gastric epithelial cells. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10216/114862 |
url |
http://hdl.handle.net/10216/114862 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0753-3322 10.1016/j.biopha.2013.08.006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799136010798366720 |