Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments

Detalhes bibliográficos
Autor(a) principal: Sousa, Maria João
Data de Publicação: 1996
Outros Autores: Estevinho, Leticia M., Côrte-Real, Manuela, Leão, Cecília
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/4395
Resumo: Cells of Zygosaccharomyces bailii ISA 1307 grown in a medium with acetic acid, ethanol, or glycerol as the sole carbon and energy source transported acetic acid by a saturable transport system. This system accepted propionic and formic acids but not lactic, sorbic, and benzoic acids. When the carbon source was glucose or fructose, the cells displayed activity of a mediated transport system specific for acetic acid, apparently not being able to recognize other monocarboxylic acids. In both types of cells, ethanol inhibited the transport of labelled acetic acid. The inhibition was noncompetitive, and the dependence of the maximum transport rate on the ethanol concentration was found to be exponential. These results reinforced the belief that, under the referenced growth conditions, the acid entered the cells mainly through a transporter protein. The simple diffusion of the undissociated acid appeared to contribute, with a relatively low weight, to the overall acid uptake. It was concluded that in Z. bailii, ethanol plays a protective role against the possible negative effects of acetic acid by inhibiting its transport and accumulation. Thus, the intracellular concentration of the acid could be maintained at levels lower than those expected if the acid entered the cells only by simple diffusion.
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spelling Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environmentsZygosaccharomyces bailiiCells of Zygosaccharomyces bailii ISA 1307 grown in a medium with acetic acid, ethanol, or glycerol as the sole carbon and energy source transported acetic acid by a saturable transport system. This system accepted propionic and formic acids but not lactic, sorbic, and benzoic acids. When the carbon source was glucose or fructose, the cells displayed activity of a mediated transport system specific for acetic acid, apparently not being able to recognize other monocarboxylic acids. In both types of cells, ethanol inhibited the transport of labelled acetic acid. The inhibition was noncompetitive, and the dependence of the maximum transport rate on the ethanol concentration was found to be exponential. These results reinforced the belief that, under the referenced growth conditions, the acid entered the cells mainly through a transporter protein. The simple diffusion of the undissociated acid appeared to contribute, with a relatively low weight, to the overall acid uptake. It was concluded that in Z. bailii, ethanol plays a protective role against the possible negative effects of acetic acid by inhibiting its transport and accumulation. Thus, the intracellular concentration of the acid could be maintained at levels lower than those expected if the acid entered the cells only by simple diffusion.American Society of MicrobiologyBiblioteca Digital do IPBSousa, Maria JoãoEstevinho, Leticia M.Côrte-Real, ManuelaLeão, Cecília2011-05-19T09:42:56Z19961996-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/4395engMaria João, Sousa; Estevinho, Letícia M.; Côrte-Real, Manuela; Leão, Cecília (1996). Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments. Applied and Environmental Microbiology. ISSN 0099-2240. 62:9, p. 3152-31570099-224010.1128/aem.62.9.3152-3157.1996info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:11:15Zoai:bibliotecadigital.ipb.pt:10198/4395Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:56:58.679051Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments
title Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments
spellingShingle Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments
Sousa, Maria João
Zygosaccharomyces bailii
title_short Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments
title_full Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments
title_fullStr Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments
title_full_unstemmed Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments
title_sort Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments
author Sousa, Maria João
author_facet Sousa, Maria João
Estevinho, Leticia M.
Côrte-Real, Manuela
Leão, Cecília
author_role author
author2 Estevinho, Leticia M.
Côrte-Real, Manuela
Leão, Cecília
author2_role author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Sousa, Maria João
Estevinho, Leticia M.
Côrte-Real, Manuela
Leão, Cecília
dc.subject.por.fl_str_mv Zygosaccharomyces bailii
topic Zygosaccharomyces bailii
description Cells of Zygosaccharomyces bailii ISA 1307 grown in a medium with acetic acid, ethanol, or glycerol as the sole carbon and energy source transported acetic acid by a saturable transport system. This system accepted propionic and formic acids but not lactic, sorbic, and benzoic acids. When the carbon source was glucose or fructose, the cells displayed activity of a mediated transport system specific for acetic acid, apparently not being able to recognize other monocarboxylic acids. In both types of cells, ethanol inhibited the transport of labelled acetic acid. The inhibition was noncompetitive, and the dependence of the maximum transport rate on the ethanol concentration was found to be exponential. These results reinforced the belief that, under the referenced growth conditions, the acid entered the cells mainly through a transporter protein. The simple diffusion of the undissociated acid appeared to contribute, with a relatively low weight, to the overall acid uptake. It was concluded that in Z. bailii, ethanol plays a protective role against the possible negative effects of acetic acid by inhibiting its transport and accumulation. Thus, the intracellular concentration of the acid could be maintained at levels lower than those expected if the acid entered the cells only by simple diffusion.
publishDate 1996
dc.date.none.fl_str_mv 1996
1996-01-01T00:00:00Z
2011-05-19T09:42:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/4395
url http://hdl.handle.net/10198/4395
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Maria João, Sousa; Estevinho, Letícia M.; Côrte-Real, Manuela; Leão, Cecília (1996). Transport of acetic acid in Zygosaccharomyces bailii: effects of ethanol and implications on the resistance of the yeast to acid environments. Applied and Environmental Microbiology. ISSN 0099-2240. 62:9, p. 3152-3157
0099-2240
10.1128/aem.62.9.3152-3157.1996
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv American Society of Microbiology
publisher.none.fl_str_mv American Society of Microbiology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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