Orange IV stabilizes silk fibroin microemulsions
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/35205 |
Resumo: | Silk fibroin (SF) is a natural biopolymer that has been extensively studied in various applications due to its impressive mechanical properties and biocompatibility. Recently, SF-based particles have been proposed as controlled drug delivery systems. A new and efficient method to prepare SF microemulsions (SF-MEs) was developed by oil-in-water emulsions using high-pressure homogenization to promote emulsification. During SF-ME production, the secondary structure of SF changed to a more stable conformation (from random coil to -sheets), thus allowing the formation of small and stable (140.7 ± 1.9 nm; polydispersity index, 0.25) SF microparticles (SF-MPs). The efficiency of SF-MP formation was 60%. Orange IV was used as a model compound for incorporation and release studies, although its incorporation into the SF-MEs significantly improved particle size and size distribution over at least 4 wk compared to traditional stabilizers (e.g., poloxamer 407, transcutol, Tween 80, and SDS). This should be a call of attention when using dyes as model compounds since they can influence particle properties and lead to misinterpretation of the results. Orange IV showed an incorporation efficiency of 91% and a controlled release over time. Stable SF-MP formulations, further enhanced by orange IV incorporation, provide an innovative method with potential application in pharmaceutical development due to its associated high biocompatibility and release profile. |
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Orange IV stabilizes silk fibroin microemulsionsHigh-pressure homogenizerMicroemulsionsMicroparticlesOrange IVSilk fibroinScience & TechnologySilk fibroin (SF) is a natural biopolymer that has been extensively studied in various applications due to its impressive mechanical properties and biocompatibility. Recently, SF-based particles have been proposed as controlled drug delivery systems. A new and efficient method to prepare SF microemulsions (SF-MEs) was developed by oil-in-water emulsions using high-pressure homogenization to promote emulsification. During SF-ME production, the secondary structure of SF changed to a more stable conformation (from random coil to -sheets), thus allowing the formation of small and stable (140.7 ± 1.9 nm; polydispersity index, 0.25) SF microparticles (SF-MPs). The efficiency of SF-MP formation was 60%. Orange IV was used as a model compound for incorporation and release studies, although its incorporation into the SF-MEs significantly improved particle size and size distribution over at least 4 wk compared to traditional stabilizers (e.g., poloxamer 407, transcutol, Tween 80, and SDS). This should be a call of attention when using dyes as model compounds since they can influence particle properties and lead to misinterpretation of the results. Orange IV showed an incorporation efficiency of 91% and a controlled release over time. Stable SF-MP formulations, further enhanced by orange IV incorporation, provide an innovative method with potential application in pharmaceutical development due to its associated high biocompatibility and release profile.The authors thank the FCT Strategic Project PEst-OE/EQB/LA0023/2013 and the project "BioHealth - Biotechnology and Bioengineering Approaches to Improve Health Quality," Ref. NORTE-07-0124-FEDER-000027, cofunded by the Programa Operacional Regional do Norte (ON.2 - O Novo Norte), QREN, FEDER. We would also like to acknowledge CRA - Unita di Ricerca di Apicoltura and Bachicoltura Padova (Italy) for the supply of B. mori silkworm cocoons, and Andreia Vasconcelos for technical support.John Wiley and SonsUniversidade do MinhoFerreira, A. V.Volkov, VadimAbreu, Ana S.Azóia, Nuno G.Botelho, C. M.Paulo, Artur Cavaco2015-052015-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/35205engFerreira, Ana V.; Volkov, V.; Abreu, A. S.; Azoia, Nuno G.; Botelho, C. M.; Paulo, Artur Cavaco, Orange IV stabilizes silk fibroin microemulsions. Engineering in Life Sciences, 15(4), 400-409, 20151618-02401618-286310.1002/elsc.201400190http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1618-2863info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:35:01ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Orange IV stabilizes silk fibroin microemulsions |
title |
Orange IV stabilizes silk fibroin microemulsions |
spellingShingle |
Orange IV stabilizes silk fibroin microemulsions Ferreira, A. V. High-pressure homogenizer Microemulsions Microparticles Orange IV Silk fibroin Science & Technology |
title_short |
Orange IV stabilizes silk fibroin microemulsions |
title_full |
Orange IV stabilizes silk fibroin microemulsions |
title_fullStr |
Orange IV stabilizes silk fibroin microemulsions |
title_full_unstemmed |
Orange IV stabilizes silk fibroin microemulsions |
title_sort |
Orange IV stabilizes silk fibroin microemulsions |
author |
Ferreira, A. V. |
author_facet |
Ferreira, A. V. Volkov, Vadim Abreu, Ana S. Azóia, Nuno G. Botelho, C. M. Paulo, Artur Cavaco |
author_role |
author |
author2 |
Volkov, Vadim Abreu, Ana S. Azóia, Nuno G. Botelho, C. M. Paulo, Artur Cavaco |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Ferreira, A. V. Volkov, Vadim Abreu, Ana S. Azóia, Nuno G. Botelho, C. M. Paulo, Artur Cavaco |
dc.subject.por.fl_str_mv |
High-pressure homogenizer Microemulsions Microparticles Orange IV Silk fibroin Science & Technology |
topic |
High-pressure homogenizer Microemulsions Microparticles Orange IV Silk fibroin Science & Technology |
description |
Silk fibroin (SF) is a natural biopolymer that has been extensively studied in various applications due to its impressive mechanical properties and biocompatibility. Recently, SF-based particles have been proposed as controlled drug delivery systems. A new and efficient method to prepare SF microemulsions (SF-MEs) was developed by oil-in-water emulsions using high-pressure homogenization to promote emulsification. During SF-ME production, the secondary structure of SF changed to a more stable conformation (from random coil to -sheets), thus allowing the formation of small and stable (140.7 ± 1.9 nm; polydispersity index, 0.25) SF microparticles (SF-MPs). The efficiency of SF-MP formation was 60%. Orange IV was used as a model compound for incorporation and release studies, although its incorporation into the SF-MEs significantly improved particle size and size distribution over at least 4 wk compared to traditional stabilizers (e.g., poloxamer 407, transcutol, Tween 80, and SDS). This should be a call of attention when using dyes as model compounds since they can influence particle properties and lead to misinterpretation of the results. Orange IV showed an incorporation efficiency of 91% and a controlled release over time. Stable SF-MP formulations, further enhanced by orange IV incorporation, provide an innovative method with potential application in pharmaceutical development due to its associated high biocompatibility and release profile. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-05 2015-05-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/35205 |
url |
http://hdl.handle.net/1822/35205 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ferreira, Ana V.; Volkov, V.; Abreu, A. S.; Azoia, Nuno G.; Botelho, C. M.; Paulo, Artur Cavaco, Orange IV stabilizes silk fibroin microemulsions. Engineering in Life Sciences, 15(4), 400-409, 2015 1618-0240 1618-2863 10.1002/elsc.201400190 http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1618-2863 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
John Wiley and Sons |
publisher.none.fl_str_mv |
John Wiley and Sons |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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1777303792437428224 |