Ligand-independent activity of the ghrelin receptor modulates AMPA receptor trafficking and supports memory formation
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/93152 https://doi.org/10.1126/scisignal.abb1953 |
Resumo: | The biological signals of hunger, satiety, and memory are interconnected. The role of the hormone ghrelin in regulating feeding and memory makes ghrelin receptors attractive targets for associated disorders. We investigated the effects of the high ligand-independent activity of the ghrelin receptor GHS-R1a on the physiology of excitatory synapses in the hippocampus. Blocking this activity produced a decrease in the synaptic content of AMPA receptors in hippocampal neurons and a reduction in GluA1 phosphorylation at Ser845. Reducing the ligand-independent activity of GHS-R1a increased the surface diffusion of AMPA receptors and impaired AMPA receptor–dependent synaptic delivery induced by chemical long-term potentiation. Accordingly, we found that blocking this GHS-R1a activity impaired spatial and recognition memory in mice. These observations support a role for the ligand-independent activity of GHS-R1a in regulating AMPA receptor trafficking under basal conditions and in the context of synaptic plasticity that underlies learning. |
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Ligand-independent activity of the ghrelin receptor modulates AMPA receptor trafficking and supports memory formationThe biological signals of hunger, satiety, and memory are interconnected. The role of the hormone ghrelin in regulating feeding and memory makes ghrelin receptors attractive targets for associated disorders. We investigated the effects of the high ligand-independent activity of the ghrelin receptor GHS-R1a on the physiology of excitatory synapses in the hippocampus. Blocking this activity produced a decrease in the synaptic content of AMPA receptors in hippocampal neurons and a reduction in GluA1 phosphorylation at Ser845. Reducing the ligand-independent activity of GHS-R1a increased the surface diffusion of AMPA receptors and impaired AMPA receptor–dependent synaptic delivery induced by chemical long-term potentiation. Accordingly, we found that blocking this GHS-R1a activity impaired spatial and recognition memory in mice. These observations support a role for the ligand-independent activity of GHS-R1a in regulating AMPA receptor trafficking under basal conditions and in the context of synaptic plasticity that underlies learning.American Association for the Advancement of Science2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/93152http://hdl.handle.net/10316/93152https://doi.org/10.1126/scisignal.abb1953eng1945-08771937-9145https://stke.sciencemag.org/content/14/670/eabb1953/tab-pdfRibeiro, Luís F.Catarino, TatianaCarvalho, MárioCortes, LuísaSantos, Sandra D.Opazo, Patricio O.Ribeiro, Lyn RosenbrierOliveiros, BárbaraChoquet, DanielEsteban, José A.Peça, JoãoCarvalho, Ana Luísainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-05-25T06:21:10Zoai:estudogeral.uc.pt:10316/93152Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:12:07.977895Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Ligand-independent activity of the ghrelin receptor modulates AMPA receptor trafficking and supports memory formation |
title |
Ligand-independent activity of the ghrelin receptor modulates AMPA receptor trafficking and supports memory formation |
spellingShingle |
Ligand-independent activity of the ghrelin receptor modulates AMPA receptor trafficking and supports memory formation Ribeiro, Luís F. |
title_short |
Ligand-independent activity of the ghrelin receptor modulates AMPA receptor trafficking and supports memory formation |
title_full |
Ligand-independent activity of the ghrelin receptor modulates AMPA receptor trafficking and supports memory formation |
title_fullStr |
Ligand-independent activity of the ghrelin receptor modulates AMPA receptor trafficking and supports memory formation |
title_full_unstemmed |
Ligand-independent activity of the ghrelin receptor modulates AMPA receptor trafficking and supports memory formation |
title_sort |
Ligand-independent activity of the ghrelin receptor modulates AMPA receptor trafficking and supports memory formation |
author |
Ribeiro, Luís F. |
author_facet |
Ribeiro, Luís F. Catarino, Tatiana Carvalho, Mário Cortes, Luísa Santos, Sandra D. Opazo, Patricio O. Ribeiro, Lyn Rosenbrier Oliveiros, Bárbara Choquet, Daniel Esteban, José A. Peça, João Carvalho, Ana Luísa |
author_role |
author |
author2 |
Catarino, Tatiana Carvalho, Mário Cortes, Luísa Santos, Sandra D. Opazo, Patricio O. Ribeiro, Lyn Rosenbrier Oliveiros, Bárbara Choquet, Daniel Esteban, José A. Peça, João Carvalho, Ana Luísa |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Luís F. Catarino, Tatiana Carvalho, Mário Cortes, Luísa Santos, Sandra D. Opazo, Patricio O. Ribeiro, Lyn Rosenbrier Oliveiros, Bárbara Choquet, Daniel Esteban, José A. Peça, João Carvalho, Ana Luísa |
description |
The biological signals of hunger, satiety, and memory are interconnected. The role of the hormone ghrelin in regulating feeding and memory makes ghrelin receptors attractive targets for associated disorders. We investigated the effects of the high ligand-independent activity of the ghrelin receptor GHS-R1a on the physiology of excitatory synapses in the hippocampus. Blocking this activity produced a decrease in the synaptic content of AMPA receptors in hippocampal neurons and a reduction in GluA1 phosphorylation at Ser845. Reducing the ligand-independent activity of GHS-R1a increased the surface diffusion of AMPA receptors and impaired AMPA receptor–dependent synaptic delivery induced by chemical long-term potentiation. Accordingly, we found that blocking this GHS-R1a activity impaired spatial and recognition memory in mice. These observations support a role for the ligand-independent activity of GHS-R1a in regulating AMPA receptor trafficking under basal conditions and in the context of synaptic plasticity that underlies learning. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/93152 http://hdl.handle.net/10316/93152 https://doi.org/10.1126/scisignal.abb1953 |
url |
http://hdl.handle.net/10316/93152 https://doi.org/10.1126/scisignal.abb1953 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1945-0877 1937-9145 https://stke.sciencemag.org/content/14/670/eabb1953/tab-pdf |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
American Association for the Advancement of Science |
publisher.none.fl_str_mv |
American Association for the Advancement of Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799134017549762560 |