Mutagenicity of PM10-bound PAHs from non-exhaust sources
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/34166 |
Resumo: | Road trafc is a major source of airborne particles. While exhaust emissions have been deeply scrutinised, non-exhaust emissions deserved less attention despite their rising contribution to particle emissions from trafc. The mutagenicity of particulate matter (PM10) samples from wear between pavements and tyres and from road dust resuspension was determined by the Ames test with two Salmonella typhimurium strains (TA98 and TA100) with or without metabolic activation. After sequential extraction of PM10 samples in dichloromethane and methanol, the extracts were separated via fash chromatography and the subfraction that contained polycyclic aromatic compounds (PAHs) was used in the assays. Additionally, the samples were analysed by gas chromatography-mass spectrometry (GC–MS). The analysed extracts contained predominantly high molecular weight PAHs with varying concentrations. The non-exhaust trafc-related source samples did not reveal mutagenic potential at the doses examined. Given the compositional complexity of the samples, aggregating multiple metals and organic compounds of diferent polarities, an integrated assessment of the toxicity of non-exhaust particles should include other in vitro toxicity assays. |
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Mutagenicity of PM10-bound PAHs from non-exhaust sourcesMutagenicityPM10Tyre wearRoad dust resuspensionPAHsAmes testRoad trafc is a major source of airborne particles. While exhaust emissions have been deeply scrutinised, non-exhaust emissions deserved less attention despite their rising contribution to particle emissions from trafc. The mutagenicity of particulate matter (PM10) samples from wear between pavements and tyres and from road dust resuspension was determined by the Ames test with two Salmonella typhimurium strains (TA98 and TA100) with or without metabolic activation. After sequential extraction of PM10 samples in dichloromethane and methanol, the extracts were separated via fash chromatography and the subfraction that contained polycyclic aromatic compounds (PAHs) was used in the assays. Additionally, the samples were analysed by gas chromatography-mass spectrometry (GC–MS). The analysed extracts contained predominantly high molecular weight PAHs with varying concentrations. The non-exhaust trafc-related source samples did not reveal mutagenic potential at the doses examined. Given the compositional complexity of the samples, aggregating multiple metals and organic compounds of diferent polarities, an integrated assessment of the toxicity of non-exhaust particles should include other in vitro toxicity assays.Springer Nature2022-042022-04-01T00:00:00Z2023-04-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/34166eng1873-931810.1007/s11869-021-01140-5Vicente, Estela DomingosLopes, IsabelFigueiredo, DanielaGonçalves, CátiaVicente, AnaAlves, Céliainfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:05:58Zoai:ria.ua.pt:10773/34166Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:05:33.668901Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Mutagenicity of PM10-bound PAHs from non-exhaust sources |
title |
Mutagenicity of PM10-bound PAHs from non-exhaust sources |
spellingShingle |
Mutagenicity of PM10-bound PAHs from non-exhaust sources Vicente, Estela Domingos Mutagenicity PM10 Tyre wear Road dust resuspension PAHs Ames test |
title_short |
Mutagenicity of PM10-bound PAHs from non-exhaust sources |
title_full |
Mutagenicity of PM10-bound PAHs from non-exhaust sources |
title_fullStr |
Mutagenicity of PM10-bound PAHs from non-exhaust sources |
title_full_unstemmed |
Mutagenicity of PM10-bound PAHs from non-exhaust sources |
title_sort |
Mutagenicity of PM10-bound PAHs from non-exhaust sources |
author |
Vicente, Estela Domingos |
author_facet |
Vicente, Estela Domingos Lopes, Isabel Figueiredo, Daniela Gonçalves, Cátia Vicente, Ana Alves, Célia |
author_role |
author |
author2 |
Lopes, Isabel Figueiredo, Daniela Gonçalves, Cátia Vicente, Ana Alves, Célia |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Vicente, Estela Domingos Lopes, Isabel Figueiredo, Daniela Gonçalves, Cátia Vicente, Ana Alves, Célia |
dc.subject.por.fl_str_mv |
Mutagenicity PM10 Tyre wear Road dust resuspension PAHs Ames test |
topic |
Mutagenicity PM10 Tyre wear Road dust resuspension PAHs Ames test |
description |
Road trafc is a major source of airborne particles. While exhaust emissions have been deeply scrutinised, non-exhaust emissions deserved less attention despite their rising contribution to particle emissions from trafc. The mutagenicity of particulate matter (PM10) samples from wear between pavements and tyres and from road dust resuspension was determined by the Ames test with two Salmonella typhimurium strains (TA98 and TA100) with or without metabolic activation. After sequential extraction of PM10 samples in dichloromethane and methanol, the extracts were separated via fash chromatography and the subfraction that contained polycyclic aromatic compounds (PAHs) was used in the assays. Additionally, the samples were analysed by gas chromatography-mass spectrometry (GC–MS). The analysed extracts contained predominantly high molecular weight PAHs with varying concentrations. The non-exhaust trafc-related source samples did not reveal mutagenic potential at the doses examined. Given the compositional complexity of the samples, aggregating multiple metals and organic compounds of diferent polarities, an integrated assessment of the toxicity of non-exhaust particles should include other in vitro toxicity assays. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04 2022-04-01T00:00:00Z 2023-04-30T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/34166 |
url |
http://hdl.handle.net/10773/34166 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1873-9318 10.1007/s11869-021-01140-5 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer Nature |
publisher.none.fl_str_mv |
Springer Nature |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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