Evaluation of three antimicrobial peptides mixtures to control the phytopathogen responsible for fire blight disease
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.11/8448 |
Resumo: | Fire blight is a severe bacterial plant disease that affects important chain-of-value fruit trees such as pear and apple trees. This disease is caused by Erwinia amylovora, a quarantine phytopathogenic bacterium, which, although highly distributed worldwide, still lacks efficient control measures. The green revolution paradigm demands sustainable agriculture practices, for which antimicrobial peptides (AMPs) have recently caught much attention. The goal of this work was to disclose the bioactivity of three peptides mixtures (BP100:RW-BP100, BP100:CA-M, and RWBP100: CA-M), against three strains of E. amylovora representing distinct genotypes and virulence (LMG 2024, Ea 630 and Ea 680). The three AMPs’ mixtures were assayed at eight different equimolar concentrations ranging from 0.25 to 6 M (1:1). Results showed MIC and MBC values between 2.5 and 4 M for every AMP mixture and strain. Regarding cell viability, flow cytometry and alamarBlue reduction, showed high reduction (>25%) of viable cells after 30 min of AMP exposure, depending on the peptide mixture and strain assayed. Hypersensitive response in tobacco plants showed that the most efficient AMPs mixtures and concentrations caused low to no reaction of the plant. Altogether, the AMPs mixtures studied are better treatment solutions to control fire blight disease than the same AMPs applied individually. |
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Evaluation of three antimicrobial peptides mixtures to control the phytopathogen responsible for fire blight diseaseAlamarBlue reductionAMPsBP100CA-MErwinia amylovoraFlow cytometryGreen revolutionHypersensitive responseRW-BP100Sustainable controlFire blight is a severe bacterial plant disease that affects important chain-of-value fruit trees such as pear and apple trees. This disease is caused by Erwinia amylovora, a quarantine phytopathogenic bacterium, which, although highly distributed worldwide, still lacks efficient control measures. The green revolution paradigm demands sustainable agriculture practices, for which antimicrobial peptides (AMPs) have recently caught much attention. The goal of this work was to disclose the bioactivity of three peptides mixtures (BP100:RW-BP100, BP100:CA-M, and RWBP100: CA-M), against three strains of E. amylovora representing distinct genotypes and virulence (LMG 2024, Ea 630 and Ea 680). The three AMPs’ mixtures were assayed at eight different equimolar concentrations ranging from 0.25 to 6 M (1:1). Results showed MIC and MBC values between 2.5 and 4 M for every AMP mixture and strain. Regarding cell viability, flow cytometry and alamarBlue reduction, showed high reduction (>25%) of viable cells after 30 min of AMP exposure, depending on the peptide mixture and strain assayed. Hypersensitive response in tobacco plants showed that the most efficient AMPs mixtures and concentrations caused low to no reaction of the plant. Altogether, the AMPs mixtures studied are better treatment solutions to control fire blight disease than the same AMPs applied individually.MDPIRepositório Científico do Instituto Politécnico de Castelo BrancoMendes, Rafael J.Sario, SaraLuz, J.P.Fagundes, NatáliaTeixeira, CátiaGomes, PaulaTavares, FernandoSantos, Conceição2023-03-28T15:13:33Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.11/8448eng10.3390/plants10122637info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-04-22T01:47:03Zoai:repositorio.ipcb.pt:10400.11/8448Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:48:12.282524Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evaluation of three antimicrobial peptides mixtures to control the phytopathogen responsible for fire blight disease |
title |
Evaluation of three antimicrobial peptides mixtures to control the phytopathogen responsible for fire blight disease |
spellingShingle |
Evaluation of three antimicrobial peptides mixtures to control the phytopathogen responsible for fire blight disease Mendes, Rafael J. AlamarBlue reduction AMPs BP100 CA-M Erwinia amylovora Flow cytometry Green revolution Hypersensitive response RW-BP100 Sustainable control |
title_short |
Evaluation of three antimicrobial peptides mixtures to control the phytopathogen responsible for fire blight disease |
title_full |
Evaluation of three antimicrobial peptides mixtures to control the phytopathogen responsible for fire blight disease |
title_fullStr |
Evaluation of three antimicrobial peptides mixtures to control the phytopathogen responsible for fire blight disease |
title_full_unstemmed |
Evaluation of three antimicrobial peptides mixtures to control the phytopathogen responsible for fire blight disease |
title_sort |
Evaluation of three antimicrobial peptides mixtures to control the phytopathogen responsible for fire blight disease |
author |
Mendes, Rafael J. |
author_facet |
Mendes, Rafael J. Sario, Sara Luz, J.P. Fagundes, Natália Teixeira, Cátia Gomes, Paula Tavares, Fernando Santos, Conceição |
author_role |
author |
author2 |
Sario, Sara Luz, J.P. Fagundes, Natália Teixeira, Cátia Gomes, Paula Tavares, Fernando Santos, Conceição |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico de Castelo Branco |
dc.contributor.author.fl_str_mv |
Mendes, Rafael J. Sario, Sara Luz, J.P. Fagundes, Natália Teixeira, Cátia Gomes, Paula Tavares, Fernando Santos, Conceição |
dc.subject.por.fl_str_mv |
AlamarBlue reduction AMPs BP100 CA-M Erwinia amylovora Flow cytometry Green revolution Hypersensitive response RW-BP100 Sustainable control |
topic |
AlamarBlue reduction AMPs BP100 CA-M Erwinia amylovora Flow cytometry Green revolution Hypersensitive response RW-BP100 Sustainable control |
description |
Fire blight is a severe bacterial plant disease that affects important chain-of-value fruit trees such as pear and apple trees. This disease is caused by Erwinia amylovora, a quarantine phytopathogenic bacterium, which, although highly distributed worldwide, still lacks efficient control measures. The green revolution paradigm demands sustainable agriculture practices, for which antimicrobial peptides (AMPs) have recently caught much attention. The goal of this work was to disclose the bioactivity of three peptides mixtures (BP100:RW-BP100, BP100:CA-M, and RWBP100: CA-M), against three strains of E. amylovora representing distinct genotypes and virulence (LMG 2024, Ea 630 and Ea 680). The three AMPs’ mixtures were assayed at eight different equimolar concentrations ranging from 0.25 to 6 M (1:1). Results showed MIC and MBC values between 2.5 and 4 M for every AMP mixture and strain. Regarding cell viability, flow cytometry and alamarBlue reduction, showed high reduction (>25%) of viable cells after 30 min of AMP exposure, depending on the peptide mixture and strain assayed. Hypersensitive response in tobacco plants showed that the most efficient AMPs mixtures and concentrations caused low to no reaction of the plant. Altogether, the AMPs mixtures studied are better treatment solutions to control fire blight disease than the same AMPs applied individually. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z 2023-03-28T15:13:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.11/8448 |
url |
http://hdl.handle.net/10400.11/8448 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.3390/plants10122637 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131565066813440 |