A novel Gardnerella, Prevotella, and Lactobacillus standard that improves accuracy in quantifying bacterial burden in vaginal microbial communities

Detalhes bibliográficos
Autor(a) principal: Elnaggar, Jacob H.
Data de Publicação: 2023
Outros Autores: Ardizzone, Caleb M., Cerca, Nuno, Toh, Evelyn, Laniewski, Pawel, Lillis, Rebecca A., Herbst-Kralovetz, Melissa M., Quayle, Alison J., Muzny, Christina A., Taylor, Christopher M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/85564
Resumo: The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2023.1198113/full#supplementary-material
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spelling A novel Gardnerella, Prevotella, and Lactobacillus standard that improves accuracy in quantifying bacterial burden in vaginal microbial communitiesbacterial burdenvaginal microbiomebacterial vaginosisbiofilmqPCR standardGardnerellaPrevotellaLactobacillusThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2023.1198113/full#supplementary-materialBacterial vaginosis (BV) is the most common vaginal dysbiosis. In this condition, a polymicrobial biofilm develops on vaginal epithelial cells. Accurately quantifying the bacterial burden of the BV biofilm is necessary to further our understanding of BV pathogenesis. Historically, the standard for calculating total bacterial burden of the BV biofilm has been based on quantifying Escherichia coli 16S rRNA gene copy number. However, E. coli is improper for measuring the bacterial burden of this unique micro-environment. Here, we propose a novel qPCR standard to quantify bacterial burden in vaginal microbial communities, from an optimal state to a mature BV biofilm. These standards consist of different combinations of vaginal bacteria including three common BV-associated bacteria (BVAB) Gardnerella spp. (G), Prevotella spp. (P), and Fannyhessea spp. (F) and commensal Lactobacillus spp. (L) using the 16S rRNA gene (G:P:F:L, G:P:F, G:P:L and 1G:9L). We compared these standards to the traditional E. coli (E) reference standard using known quantities of mock vaginal communities and 16 vaginal samples from women. The E standard significantly underestimated the copy numbers of the mock communities, and this underestimation was significantly greater at lower copy numbers of these communities. The G:P:L standard was the most accurate across all mock communities and when compared to other mixed vaginal standards. Mixed vaginal standards were further validated with vaginal samples. This new G:P:L standard can be used in BV pathogenesis research to enhance reproducibility and reliability in quantitative measurements of BVAB, spanning from the optimal to non-optimal (including BV) vaginal microbiota.This work was supported by the National Institute of Allergy and Infectious Diseases (grants R01AI146065 to CM and R01AI118860 to AQ and CT), National Center for Advancing Translational Sciences (grant UL1TR003096 to the University of Alabama-Birmingham Center for Clinical and Translational Science), and an American Sexually Transmitted Diseases Association Small Project Assistance award (to JE).info:eu-repo/semantics/publishedVersionFrontiers Media S.A.Universidade do MinhoElnaggar, Jacob H.Ardizzone, Caleb M.Cerca, NunoToh, EvelynLaniewski, PawelLillis, Rebecca A.Herbst-Kralovetz, Melissa M.Quayle, Alison J.Muzny, Christina A.Taylor, Christopher M.2023-03-192023-03-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/85564engElnaggar, J. H., Ardizzone, C. M., Cerca, N., Toh, E., Łaniewski, P., Lillis, R. A., … Taylor, C. M. (2023, June 19). A novel Gardnerella, Prevotella, and Lactobacillus standard that improves accuracy in quantifying bacterial burden in vaginal microbial communities. Frontiers in Cellular and Infection Microbiology. Frontiers Media SA. http://doi.org/10.3389/fcimb.2023.11981132235-298810.3389/fcimb.2023.119811337404722https://www.frontiersin.org/articles/10.3389/fcimb.2023.1198113/fullinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-12T01:17:35Zoai:repositorium.sdum.uminho.pt:1822/85564Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:11:40.326929Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A novel Gardnerella, Prevotella, and Lactobacillus standard that improves accuracy in quantifying bacterial burden in vaginal microbial communities
title A novel Gardnerella, Prevotella, and Lactobacillus standard that improves accuracy in quantifying bacterial burden in vaginal microbial communities
spellingShingle A novel Gardnerella, Prevotella, and Lactobacillus standard that improves accuracy in quantifying bacterial burden in vaginal microbial communities
Elnaggar, Jacob H.
bacterial burden
vaginal microbiome
bacterial vaginosis
biofilm
qPCR standard
Gardnerella
Prevotella
Lactobacillus
title_short A novel Gardnerella, Prevotella, and Lactobacillus standard that improves accuracy in quantifying bacterial burden in vaginal microbial communities
title_full A novel Gardnerella, Prevotella, and Lactobacillus standard that improves accuracy in quantifying bacterial burden in vaginal microbial communities
title_fullStr A novel Gardnerella, Prevotella, and Lactobacillus standard that improves accuracy in quantifying bacterial burden in vaginal microbial communities
title_full_unstemmed A novel Gardnerella, Prevotella, and Lactobacillus standard that improves accuracy in quantifying bacterial burden in vaginal microbial communities
title_sort A novel Gardnerella, Prevotella, and Lactobacillus standard that improves accuracy in quantifying bacterial burden in vaginal microbial communities
author Elnaggar, Jacob H.
author_facet Elnaggar, Jacob H.
Ardizzone, Caleb M.
Cerca, Nuno
Toh, Evelyn
Laniewski, Pawel
Lillis, Rebecca A.
Herbst-Kralovetz, Melissa M.
Quayle, Alison J.
Muzny, Christina A.
Taylor, Christopher M.
author_role author
author2 Ardizzone, Caleb M.
Cerca, Nuno
Toh, Evelyn
Laniewski, Pawel
Lillis, Rebecca A.
Herbst-Kralovetz, Melissa M.
Quayle, Alison J.
Muzny, Christina A.
Taylor, Christopher M.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Elnaggar, Jacob H.
Ardizzone, Caleb M.
Cerca, Nuno
Toh, Evelyn
Laniewski, Pawel
Lillis, Rebecca A.
Herbst-Kralovetz, Melissa M.
Quayle, Alison J.
Muzny, Christina A.
Taylor, Christopher M.
dc.subject.por.fl_str_mv bacterial burden
vaginal microbiome
bacterial vaginosis
biofilm
qPCR standard
Gardnerella
Prevotella
Lactobacillus
topic bacterial burden
vaginal microbiome
bacterial vaginosis
biofilm
qPCR standard
Gardnerella
Prevotella
Lactobacillus
description The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2023.1198113/full#supplementary-material
publishDate 2023
dc.date.none.fl_str_mv 2023-03-19
2023-03-19T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/85564
url https://hdl.handle.net/1822/85564
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Elnaggar, J. H., Ardizzone, C. M., Cerca, N., Toh, E., Łaniewski, P., Lillis, R. A., … Taylor, C. M. (2023, June 19). A novel Gardnerella, Prevotella, and Lactobacillus standard that improves accuracy in quantifying bacterial burden in vaginal microbial communities. Frontiers in Cellular and Infection Microbiology. Frontiers Media SA. http://doi.org/10.3389/fcimb.2023.1198113
2235-2988
10.3389/fcimb.2023.1198113
37404722
https://www.frontiersin.org/articles/10.3389/fcimb.2023.1198113/full
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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