Detection of drug resistant mycobacterium tuberculosis strains using kit SIRE nitratase®

• Autor(a) principal: de Miranda, Silvana Spindola
• Data de Publicação: 2020
• Outros Autores: de Almeida, Isabela Neves, de Fátima Filardi Oliveira Mansur, Maria, de Assis Figueredo, Lida Jouca, da Silva Carvalho, Wânia, Hadaad, João Paulo Amaral, de Lourdes do Carmo Guimarães Diniz, Jaciara, von Groll, Andrea, da Silva, Pedro Almeida, Lopes, Maria Luiza, dos Santos, Marcelo Cordeiro, Brito, Alexandra, de Queiroz Mello, Fernanda Carvalho, da Silva Santos Malaquias, Thiago, Croda, Julio, Pinhata, Juliana Maira Watanabe, de Oliveira, Rosângela Siqueira, Chimara, Erica, Rossetti, Maria Lúcia, Halon, Maria Laura, Lourenço, Maria Cristina, de Melo Medeiros, Reginalda Ferreira, Montes, Fátima Cristina Onofre Fandinho, Machado, Diana, Viveiros, Miguel, Kritski, Afrânio Lineu
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10362/116609
• topic: MDR; Nitrate reductase; Technology; Tuberculosis; Biochemistry, Genetics and Molecular Biology (miscellaneous); Infectious Diseases; Microbiology; SDG 3 - Good Health and Well-being

(1) Background: The Commercial Kit SIRE Nitratase® PlastLabor, is a drug susceptibility test kit used to detect Mycobacterium tuberculosis resistance to first-line TB treatment drugs. The present study aimed at evaluating its performance in a multicenter study. (2) Methods: To determine its accuracy, the proportion methods in Lowenstein Jensen medium or the BACTECTMMGITTM960 system was used as a gold standard. (3) Results: The study revealed that the respective accuracies of the kit with 190 M. tuberculosis clinical isolates, using the proportion methods in Lowenstein Jensen medium or BACTECTMMGITTM960 system as a gold standard, were 93.9% and 94.6%, 96.9% and 94.6%, 98.0% and 97.8%, and 98.0% and 98.9%, for streptomycin, isoniazid, rifampicin, and ethambutol, respectively. (4) Conclusion: Thus, the kit can rapidly screen resistance to streptomycin, isoniazid, rifampicin, and ethambutol. Additionally, it does not require sophisticated equipment; hence, it can be easily used in the laboratories of low and middle income countries.