Identificação de genes expressos durante a interacção cv. florina - v. inaequalis por differential display-rt-pcr

Detalhes bibliográficos
Autor(a) principal: Oliveira, Cristina Moniz
Data de Publicação: 2008
Outros Autores: Mota, Mariana
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/1020
Resumo: In order to identify genes that are specifically regulated during the expression of the resistance mechanism of the apple cv. Florina towards the apple scab fungus, the genetic expression pattern in plants inoculated with a conidial suspension of Venturia inaequalis was compared with the expression pattern in plants inoculated with water, by differential display RT-PCR. Amplified products derived from the combination of one oligodT (dTVG) with 20 10-mer primers were separated in polyacrylamide denaturing gel and detected with silver nitrate. 14 cDNA fragments that were only visible in the sample inoculated with fungal suspension, or more abundant in this one, were purified from the gel for reamplification and cloning. Three fragments were already sequenced and one sequence showed very strong homology with a plant phytochelatin synthetase, that might be involved in stress responses. Differential accumulation of this cDNA upon fungal inoculation shall be verified by reverse-transcription PCR with specific primers.
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spelling Identificação de genes expressos durante a interacção cv. florina - v. inaequalis por differential display-rt-pcrIdentification of genes expressed during the interaction between cv. florina and v. inaequalis by differential display-rt-pcrapple scabdifferential display-rt-pcrFlorinaphytochelatin synthetaseexpressão diferencialpedradomacieirafitoquelatina sintetaseIn order to identify genes that are specifically regulated during the expression of the resistance mechanism of the apple cv. Florina towards the apple scab fungus, the genetic expression pattern in plants inoculated with a conidial suspension of Venturia inaequalis was compared with the expression pattern in plants inoculated with water, by differential display RT-PCR. Amplified products derived from the combination of one oligodT (dTVG) with 20 10-mer primers were separated in polyacrylamide denaturing gel and detected with silver nitrate. 14 cDNA fragments that were only visible in the sample inoculated with fungal suspension, or more abundant in this one, were purified from the gel for reamplification and cloning. Three fragments were already sequenced and one sequence showed very strong homology with a plant phytochelatin synthetase, that might be involved in stress responses. Differential accumulation of this cDNA upon fungal inoculation shall be verified by reverse-transcription PCR with specific primers.Sociedade de Ciências Agrárias de PortugalRepositório da Universidade de LisboaOliveira, Cristina MonizMota, Mariana2009-06-05T15:01:19Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/1020por"Revista de Ciências Agrárias". ISSN 0871-018X. 31:2 (2008) 139-1470871-018Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-10T01:33:34Zoai:www.repository.utl.pt:10400.5/1020Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:49:14.673173Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Identificação de genes expressos durante a interacção cv. florina - v. inaequalis por differential display-rt-pcr
Identification of genes expressed during the interaction between cv. florina and v. inaequalis by differential display-rt-pcr
title Identificação de genes expressos durante a interacção cv. florina - v. inaequalis por differential display-rt-pcr
spellingShingle Identificação de genes expressos durante a interacção cv. florina - v. inaequalis por differential display-rt-pcr
Oliveira, Cristina Moniz
apple scab
differential display-rt-pcr
Florina
phytochelatin synthetase
expressão diferencial
pedrado
macieira
fitoquelatina sintetase
title_short Identificação de genes expressos durante a interacção cv. florina - v. inaequalis por differential display-rt-pcr
title_full Identificação de genes expressos durante a interacção cv. florina - v. inaequalis por differential display-rt-pcr
title_fullStr Identificação de genes expressos durante a interacção cv. florina - v. inaequalis por differential display-rt-pcr
title_full_unstemmed Identificação de genes expressos durante a interacção cv. florina - v. inaequalis por differential display-rt-pcr
title_sort Identificação de genes expressos durante a interacção cv. florina - v. inaequalis por differential display-rt-pcr
author Oliveira, Cristina Moniz
author_facet Oliveira, Cristina Moniz
Mota, Mariana
author_role author
author2 Mota, Mariana
author2_role author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Oliveira, Cristina Moniz
Mota, Mariana
dc.subject.por.fl_str_mv apple scab
differential display-rt-pcr
Florina
phytochelatin synthetase
expressão diferencial
pedrado
macieira
fitoquelatina sintetase
topic apple scab
differential display-rt-pcr
Florina
phytochelatin synthetase
expressão diferencial
pedrado
macieira
fitoquelatina sintetase
description In order to identify genes that are specifically regulated during the expression of the resistance mechanism of the apple cv. Florina towards the apple scab fungus, the genetic expression pattern in plants inoculated with a conidial suspension of Venturia inaequalis was compared with the expression pattern in plants inoculated with water, by differential display RT-PCR. Amplified products derived from the combination of one oligodT (dTVG) with 20 10-mer primers were separated in polyacrylamide denaturing gel and detected with silver nitrate. 14 cDNA fragments that were only visible in the sample inoculated with fungal suspension, or more abundant in this one, were purified from the gel for reamplification and cloning. Three fragments were already sequenced and one sequence showed very strong homology with a plant phytochelatin synthetase, that might be involved in stress responses. Differential accumulation of this cDNA upon fungal inoculation shall be verified by reverse-transcription PCR with specific primers.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2009-06-05T15:01:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/1020
url http://hdl.handle.net/10400.5/1020
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv "Revista de Ciências Agrárias". ISSN 0871-018X. 31:2 (2008) 139-147
0871-018X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade de Ciências Agrárias de Portugal
publisher.none.fl_str_mv Sociedade de Ciências Agrárias de Portugal
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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