Production of bacterial biopolymers from industrial fat-containing wastes

Detalhes bibliográficos
Autor(a) principal: Morais, Cristiana Carvalho
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/10922
Resumo: Dissertation presented in partial fulfilment of the Requirements for the Degree of Master in Biotechnology
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spelling Production of bacterial biopolymers from industrial fat-containing wastesFatty acidsWastePoly(3-hydroxybutyrate)Medium-chainlength polyhydroxyalkanoates (mcl-PHAs)Cupriavidus necatorPseudomonas speciesDissertation presented in partial fulfilment of the Requirements for the Degree of Master in BiotechnologyPolyhydroxyalkanoates (PHAs) constitute a group of biobased and biodegradable polymers, which have been recognized as good substitutes for petroleum-based polymers in many applications. The large-scale production of PHAs is limited by the high cost of the most commonly used carbon sources (e.g. glucose, sucrose). However, the food industry generates large amounts of wastes, including fat-containing materials that can be used as low cost carbon sources for microbial cultivation, due their high carbon content. In this study, several bacterial strains (Cupriavidus necator, Comamonas testosteroni, Pseudomonas oleovorans, P. resinovorans, P. stutzeri, and P. citronellolis) were evaluated for their ability to grow and produce PHAs using fat-containing wastes generated by the food industry. The materials used in this study were mainly composed of free fatty acids, namely mystiric, oleic, linoleic and stearic acid. In the preliminary shake flask experiments, C. necator, C. testosteroni, P. oleovorans and P. citronellolis were able to grow and produce PHA polymer on margarine waste with the highest content. Those strains were selected for batch bioreactor experiments, wherein C. necator reached the highest polymer content (56%, wt/wt) and volumetric productivity (0.33 gPHA/L.h), Lower PHA contents were achieved by P. citronellolis and P. oleovorans (7.0 and 8.5%, wt/wt, respectively). However, in contrast with C. necator that synthesized polyhydroxybutyrate [P3(HB)], those strains produced medium chain length polyesters (mcl-PHA) containing monomers of 3-hydroxyoctanoate (HO) and 3-hydroxydecanoate (HD). C. necator was also cultivated in two different fed-batch strategies. The first cultivation achieved 76% (wt/wt) of P(3HB), while high cell densities were obtained in the second cultivation (48 g/L of active biomass concentration). Finally, the P(3HB) and mcl-PHA polymers had a glass transition temperature of 0.5–7.9ºC and -45.6, a melting point of 169.3–173.4ºC and 60.9ºC, and degree of crystallinity of 48.7–56.6% and 0.7%, respectively.Faculdade de Ciências e TecnologiaFreitas, Maria FilomenaReis, Maria d'AscensãoRUNMorais, Cristiana Carvalho2014-01-02T10:43:57Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/10922TID:201755483enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-10T15:30:05ZPortal AgregadorONG
dc.title.none.fl_str_mv Production of bacterial biopolymers from industrial fat-containing wastes
title Production of bacterial biopolymers from industrial fat-containing wastes
spellingShingle Production of bacterial biopolymers from industrial fat-containing wastes
Morais, Cristiana Carvalho
Fatty acids
Waste
Poly(3-hydroxybutyrate)
Medium-chainlength polyhydroxyalkanoates (mcl-PHAs)
Cupriavidus necator
Pseudomonas species
title_short Production of bacterial biopolymers from industrial fat-containing wastes
title_full Production of bacterial biopolymers from industrial fat-containing wastes
title_fullStr Production of bacterial biopolymers from industrial fat-containing wastes
title_full_unstemmed Production of bacterial biopolymers from industrial fat-containing wastes
title_sort Production of bacterial biopolymers from industrial fat-containing wastes
author Morais, Cristiana Carvalho
author_facet Morais, Cristiana Carvalho
author_role author
dc.contributor.none.fl_str_mv Freitas, Maria Filomena
Reis, Maria d'Ascensão
RUN
dc.contributor.author.fl_str_mv Morais, Cristiana Carvalho
dc.subject.por.fl_str_mv Fatty acids
Waste
Poly(3-hydroxybutyrate)
Medium-chainlength polyhydroxyalkanoates (mcl-PHAs)
Cupriavidus necator
Pseudomonas species
topic Fatty acids
Waste
Poly(3-hydroxybutyrate)
Medium-chainlength polyhydroxyalkanoates (mcl-PHAs)
Cupriavidus necator
Pseudomonas species
description Dissertation presented in partial fulfilment of the Requirements for the Degree of Master in Biotechnology
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
2014-01-02T10:43:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/10922
TID:201755483
url http://hdl.handle.net/10362/10922
identifier_str_mv TID:201755483
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv
repository.mail.fl_str_mv
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