Validation of the rat model of prostate cancer: correlating seminal vesicle lesions with dorsolateral prostate lesions
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/34250 https://doi.org/10.21873/invivo.13001 |
Resumo: | Background/aim: Lesions in the seminal vesicle are described in the most used protocols for prostate cancer (PCa) induction. This study aimed to characterize the lesions of seminal vesicles associated with a protocol of PCa induction in rats to contribute to better characterization of this model. Materials and methods: Forty-five male Wistar Unilever rats were randomly divided into two control groups: CONT1 (n=10) and CONT2 (n=10); and two PCa-induced groups: IND1 (n=10) and IND2 (n=15), sacrificed at 35 and 61 weeks, respectively. Animals from the induced groups were exposed to a multistep protocol for PCa induction. Animals, seminal vesicles and dorsolateral prostate were weighed. Seminal vesicles and dorsolateral prostate were submitted to histopathological and immunohistochemical analysis. Results: Animals in which PCa was induced had a lower mean body weight when compared with the control animals (p<0.05). The relative mean seminal vesicle weight was higher in groups with PCa when compared with control groups (p<0.05). Although the differences were not statistically significant, animals from the IND2 group developed more lesions than animals from the IND1 and CONT2 groups. It is worth noting that the animals from group IND2 developed papillary adenomas and carcinomas in situ, which were not observed in any other group. Similar to observations in seminal vesicles, animals from group IND2 developed more dorsolateral prostate lesions than animals from the IND1 group (p<0.05). Conclusion: We observed that the longer the exposure to testosterone was, the greater was the incidence of preneoplastic and neoplastic lesions in both the seminal vesicle and the prostate, suggesting that testosterone exposure affects the spectrum of developed lesions. |
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Validation of the rat model of prostate cancer: correlating seminal vesicle lesions with dorsolateral prostate lesionsAnimal modelCancerProstateBackground/aim: Lesions in the seminal vesicle are described in the most used protocols for prostate cancer (PCa) induction. This study aimed to characterize the lesions of seminal vesicles associated with a protocol of PCa induction in rats to contribute to better characterization of this model. Materials and methods: Forty-five male Wistar Unilever rats were randomly divided into two control groups: CONT1 (n=10) and CONT2 (n=10); and two PCa-induced groups: IND1 (n=10) and IND2 (n=15), sacrificed at 35 and 61 weeks, respectively. Animals from the induced groups were exposed to a multistep protocol for PCa induction. Animals, seminal vesicles and dorsolateral prostate were weighed. Seminal vesicles and dorsolateral prostate were submitted to histopathological and immunohistochemical analysis. Results: Animals in which PCa was induced had a lower mean body weight when compared with the control animals (p<0.05). The relative mean seminal vesicle weight was higher in groups with PCa when compared with control groups (p<0.05). Although the differences were not statistically significant, animals from the IND2 group developed more lesions than animals from the IND1 and CONT2 groups. It is worth noting that the animals from group IND2 developed papillary adenomas and carcinomas in situ, which were not observed in any other group. Similar to observations in seminal vesicles, animals from group IND2 developed more dorsolateral prostate lesions than animals from the IND1 group (p<0.05). Conclusion: We observed that the longer the exposure to testosterone was, the greater was the incidence of preneoplastic and neoplastic lesions in both the seminal vesicle and the prostate, suggesting that testosterone exposure affects the spectrum of developed lesions.In vivo2023-02-13T17:24:14Z2023-02-132022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/34250http://hdl.handle.net/10174/34250https://doi.org/10.21873/invivo.13001engNascimento-Gonçalves E, Seixas F, Oliveira BMM, Teiga-Teixeira JE, Faustino-Rocha AI, Colaço B, Ferreira R, Oliveira PA. Validation of the rat model of prostate cancer: correlating seminal vesicle lesions with dorsolateral prostate lesions. In vivo 36(6): 2662-2668. Doi: 10.21873/invivo.13001.https://pubmed.ncbi.nlm.nih.gov/36309374/ndndndndndndndndNascimento-Gonçalves, ElisabeteSeixas, FernandaOliveira, Bruno MMTeiga-Teixeira, JoãoFaustino-Rocha, Ana IColaço, BrunoFerreira, RitaOliveira, Paula A.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:34:38Zoai:dspace.uevora.pt:10174/34250Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:22:01.602530Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Validation of the rat model of prostate cancer: correlating seminal vesicle lesions with dorsolateral prostate lesions |
title |
Validation of the rat model of prostate cancer: correlating seminal vesicle lesions with dorsolateral prostate lesions |
spellingShingle |
Validation of the rat model of prostate cancer: correlating seminal vesicle lesions with dorsolateral prostate lesions Nascimento-Gonçalves, Elisabete Animal model Cancer Prostate |
title_short |
Validation of the rat model of prostate cancer: correlating seminal vesicle lesions with dorsolateral prostate lesions |
title_full |
Validation of the rat model of prostate cancer: correlating seminal vesicle lesions with dorsolateral prostate lesions |
title_fullStr |
Validation of the rat model of prostate cancer: correlating seminal vesicle lesions with dorsolateral prostate lesions |
title_full_unstemmed |
Validation of the rat model of prostate cancer: correlating seminal vesicle lesions with dorsolateral prostate lesions |
title_sort |
Validation of the rat model of prostate cancer: correlating seminal vesicle lesions with dorsolateral prostate lesions |
author |
Nascimento-Gonçalves, Elisabete |
author_facet |
Nascimento-Gonçalves, Elisabete Seixas, Fernanda Oliveira, Bruno MM Teiga-Teixeira, João Faustino-Rocha, Ana I Colaço, Bruno Ferreira, Rita Oliveira, Paula A. |
author_role |
author |
author2 |
Seixas, Fernanda Oliveira, Bruno MM Teiga-Teixeira, João Faustino-Rocha, Ana I Colaço, Bruno Ferreira, Rita Oliveira, Paula A. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Nascimento-Gonçalves, Elisabete Seixas, Fernanda Oliveira, Bruno MM Teiga-Teixeira, João Faustino-Rocha, Ana I Colaço, Bruno Ferreira, Rita Oliveira, Paula A. |
dc.subject.por.fl_str_mv |
Animal model Cancer Prostate |
topic |
Animal model Cancer Prostate |
description |
Background/aim: Lesions in the seminal vesicle are described in the most used protocols for prostate cancer (PCa) induction. This study aimed to characterize the lesions of seminal vesicles associated with a protocol of PCa induction in rats to contribute to better characterization of this model. Materials and methods: Forty-five male Wistar Unilever rats were randomly divided into two control groups: CONT1 (n=10) and CONT2 (n=10); and two PCa-induced groups: IND1 (n=10) and IND2 (n=15), sacrificed at 35 and 61 weeks, respectively. Animals from the induced groups were exposed to a multistep protocol for PCa induction. Animals, seminal vesicles and dorsolateral prostate were weighed. Seminal vesicles and dorsolateral prostate were submitted to histopathological and immunohistochemical analysis. Results: Animals in which PCa was induced had a lower mean body weight when compared with the control animals (p<0.05). The relative mean seminal vesicle weight was higher in groups with PCa when compared with control groups (p<0.05). Although the differences were not statistically significant, animals from the IND2 group developed more lesions than animals from the IND1 and CONT2 groups. It is worth noting that the animals from group IND2 developed papillary adenomas and carcinomas in situ, which were not observed in any other group. Similar to observations in seminal vesicles, animals from group IND2 developed more dorsolateral prostate lesions than animals from the IND1 group (p<0.05). Conclusion: We observed that the longer the exposure to testosterone was, the greater was the incidence of preneoplastic and neoplastic lesions in both the seminal vesicle and the prostate, suggesting that testosterone exposure affects the spectrum of developed lesions. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01T00:00:00Z 2023-02-13T17:24:14Z 2023-02-13 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/34250 http://hdl.handle.net/10174/34250 https://doi.org/10.21873/invivo.13001 |
url |
http://hdl.handle.net/10174/34250 https://doi.org/10.21873/invivo.13001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nascimento-Gonçalves E, Seixas F, Oliveira BMM, Teiga-Teixeira JE, Faustino-Rocha AI, Colaço B, Ferreira R, Oliveira PA. Validation of the rat model of prostate cancer: correlating seminal vesicle lesions with dorsolateral prostate lesions. In vivo 36(6): 2662-2668. Doi: 10.21873/invivo.13001. https://pubmed.ncbi.nlm.nih.gov/36309374/ nd nd nd nd nd nd nd nd |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
In vivo |
publisher.none.fl_str_mv |
In vivo |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136701587652608 |