Continuous production of dietetic structured lipids using crude acidic Olive pomace oils
Main Author: | |
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Publication Date: | 2023 |
Other Authors: | , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/10400.5/30280 |
Summary: | Crude olive pomace oil (OPO) is a by-product of olive oil extraction. In this study, lowcalorie structured triacylglycerols (TAGs) were produced by acidolysis of crude OPO with mediumchain fatty acids (caprylic, C8:0; capric, C10:0) or interesterification with their ethyl ester forms (C8EE, C10EE). These new TAGs present long-chain fatty acids (L) at position sn-2 and medium-chain fatty acids (M) at positions sn-1,3 (MLM). Crude OPO exhibited a high acidity (12.05–28.75% free fatty acids), and high contents of chlorophylls and oxidation products. Reactions were carried out continuously in a packed-bed bioreactor for 70 h, using sn-1,3 regioselective commercial immobilized lipases (Thermomyces lanuginosus lipase, Lipozyme TL IM; and Rhizomucor miehei lipase, Lipozyme RM IM), in solvent-free media at 40 C. Lipozyme RM IM presented a higher affinity for C10:0 and C10EE. Lipozyme TL IM preferred C10:0 over C8:0 but C8EE over C10EE. Both biocatalysts showed a high activity and operational stability and were not affected by OPO acidity. The New TAG yields ranged 30–60 and the specific productivity ranged 0.96–1.87 g NewTAG/h.g biocatalyst. Lipozyme RM IM cost is more than seven-fold the Lipozyme TL IM cost. Therefore, using Lipozyme TL IM and crude acidic OPO in a continuous bioreactor will contribute to process sustainability for structured lipid production by lowering the cost of the biocatalyst and avoiding oil refining |
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Continuous production of dietetic structured lipids using crude acidic Olive pomace oilsacidolysisbiocatalysiscontinuous bioreactordietetic lipidinteresterificationlipaselow-calorie fatolive pomace oilstructured lipidsstructured triacylglycerolsCrude olive pomace oil (OPO) is a by-product of olive oil extraction. In this study, lowcalorie structured triacylglycerols (TAGs) were produced by acidolysis of crude OPO with mediumchain fatty acids (caprylic, C8:0; capric, C10:0) or interesterification with their ethyl ester forms (C8EE, C10EE). These new TAGs present long-chain fatty acids (L) at position sn-2 and medium-chain fatty acids (M) at positions sn-1,3 (MLM). Crude OPO exhibited a high acidity (12.05–28.75% free fatty acids), and high contents of chlorophylls and oxidation products. Reactions were carried out continuously in a packed-bed bioreactor for 70 h, using sn-1,3 regioselective commercial immobilized lipases (Thermomyces lanuginosus lipase, Lipozyme TL IM; and Rhizomucor miehei lipase, Lipozyme RM IM), in solvent-free media at 40 C. Lipozyme RM IM presented a higher affinity for C10:0 and C10EE. Lipozyme TL IM preferred C10:0 over C8:0 but C8EE over C10EE. Both biocatalysts showed a high activity and operational stability and were not affected by OPO acidity. The New TAG yields ranged 30–60 and the specific productivity ranged 0.96–1.87 g NewTAG/h.g biocatalyst. Lipozyme RM IM cost is more than seven-fold the Lipozyme TL IM cost. Therefore, using Lipozyme TL IM and crude acidic OPO in a continuous bioreactor will contribute to process sustainability for structured lipid production by lowering the cost of the biocatalyst and avoiding oil refiningMDPIRepositório da Universidade de LisboaSouza-Gonçalves, JoanaFialho, ArsénioSoares, Cleide M. F.Osório, Natália M.Ferreira-Dias, Suzana2024-03-05T18:42:40Z2023-032023-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/30280engSouza-Gonçalves, J.; Fialho, A.; Soares, C.M.F.; Osório, N.M.; Ferreira-Dias, S. Continuous production of dietetic structured lipids using crude acidic Olive pomace oils. Molecules 2023, 28, 263710.3390/molecules28062637info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-10T01:34:41Zoai:www.repository.utl.pt:10400.5/30280Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:14:15.896943Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Continuous production of dietetic structured lipids using crude acidic Olive pomace oils |
title |
Continuous production of dietetic structured lipids using crude acidic Olive pomace oils |
spellingShingle |
Continuous production of dietetic structured lipids using crude acidic Olive pomace oils Souza-Gonçalves, Joana acidolysis biocatalysis continuous bioreactor dietetic lipid interesterification lipase low-calorie fat olive pomace oil structured lipids structured triacylglycerols |
title_short |
Continuous production of dietetic structured lipids using crude acidic Olive pomace oils |
title_full |
Continuous production of dietetic structured lipids using crude acidic Olive pomace oils |
title_fullStr |
Continuous production of dietetic structured lipids using crude acidic Olive pomace oils |
title_full_unstemmed |
Continuous production of dietetic structured lipids using crude acidic Olive pomace oils |
title_sort |
Continuous production of dietetic structured lipids using crude acidic Olive pomace oils |
author |
Souza-Gonçalves, Joana |
author_facet |
Souza-Gonçalves, Joana Fialho, Arsénio Soares, Cleide M. F. Osório, Natália M. Ferreira-Dias, Suzana |
author_role |
author |
author2 |
Fialho, Arsénio Soares, Cleide M. F. Osório, Natália M. Ferreira-Dias, Suzana |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Souza-Gonçalves, Joana Fialho, Arsénio Soares, Cleide M. F. Osório, Natália M. Ferreira-Dias, Suzana |
dc.subject.por.fl_str_mv |
acidolysis biocatalysis continuous bioreactor dietetic lipid interesterification lipase low-calorie fat olive pomace oil structured lipids structured triacylglycerols |
topic |
acidolysis biocatalysis continuous bioreactor dietetic lipid interesterification lipase low-calorie fat olive pomace oil structured lipids structured triacylglycerols |
description |
Crude olive pomace oil (OPO) is a by-product of olive oil extraction. In this study, lowcalorie structured triacylglycerols (TAGs) were produced by acidolysis of crude OPO with mediumchain fatty acids (caprylic, C8:0; capric, C10:0) or interesterification with their ethyl ester forms (C8EE, C10EE). These new TAGs present long-chain fatty acids (L) at position sn-2 and medium-chain fatty acids (M) at positions sn-1,3 (MLM). Crude OPO exhibited a high acidity (12.05–28.75% free fatty acids), and high contents of chlorophylls and oxidation products. Reactions were carried out continuously in a packed-bed bioreactor for 70 h, using sn-1,3 regioselective commercial immobilized lipases (Thermomyces lanuginosus lipase, Lipozyme TL IM; and Rhizomucor miehei lipase, Lipozyme RM IM), in solvent-free media at 40 C. Lipozyme RM IM presented a higher affinity for C10:0 and C10EE. Lipozyme TL IM preferred C10:0 over C8:0 but C8EE over C10EE. Both biocatalysts showed a high activity and operational stability and were not affected by OPO acidity. The New TAG yields ranged 30–60 and the specific productivity ranged 0.96–1.87 g NewTAG/h.g biocatalyst. Lipozyme RM IM cost is more than seven-fold the Lipozyme TL IM cost. Therefore, using Lipozyme TL IM and crude acidic OPO in a continuous bioreactor will contribute to process sustainability for structured lipid production by lowering the cost of the biocatalyst and avoiding oil refining |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-03 2023-03-01T00:00:00Z 2024-03-05T18:42:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/30280 |
url |
http://hdl.handle.net/10400.5/30280 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Souza-Gonçalves, J.; Fialho, A.; Soares, C.M.F.; Osório, N.M.; Ferreira-Dias, S. Continuous production of dietetic structured lipids using crude acidic Olive pomace oils. Molecules 2023, 28, 2637 10.3390/molecules28062637 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
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