A kinetic model for curcumin production in Escherichia coli

Detalhes bibliográficos
Autor(a) principal: Machado, C. D.
Data de Publicação: 2014
Outros Autores: Rodrigues, L. R., Rocha, I.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/31617
Resumo: Curcumin is a natural compound obtained from turmeric, and is well known for its pharmacological effects. In this work, we design a heterologous pathway for industrial production of curcumin in Escherichia coli. A kinetic model of the pathway is then developed and connected to a kinetic model of the central carbon metabolism of E. coli. This model is used for optimization of the mutant strain through a rational design approach, and two manipulation targets are identified for overexpression. Dynamic simulations are then performed to compare the curcumin production profiles of the different mutant strains. Our results show that it is possible to obtain a significant improvement in the curcumin production rates with the proposed mutants. The kinetic model here developed can be an important framework to optimize curcumin production at an industrial scale and add value to its biomedical potential.
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spelling A kinetic model for curcumin production in Escherichia coliMetabolic engineeringKinetic modelingEscherichia coliCurcuminScience & TechnologyCurcumin is a natural compound obtained from turmeric, and is well known for its pharmacological effects. In this work, we design a heterologous pathway for industrial production of curcumin in Escherichia coli. A kinetic model of the pathway is then developed and connected to a kinetic model of the central carbon metabolism of E. coli. This model is used for optimization of the mutant strain through a rational design approach, and two manipulation targets are identified for overexpression. Dynamic simulations are then performed to compare the curcumin production profiles of the different mutant strains. Our results show that it is possible to obtain a significant improvement in the curcumin production rates with the proposed mutants. The kinetic model here developed can be an important framework to optimize curcumin production at an industrial scale and add value to its biomedical potential.Research supported by the Portuguese Foundation for Science and Technology (FCT), through the project "SYNBIOBACTHER - Synthetic biology approaches to engineer therapeutic bacteria" (PTDC/EBB-BIO/102863/2008).ElsevierUniversidade do MinhoMachado, C. D.Rodrigues, L. R.Rocha, I.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31617eng0303-264710.1016/j.biosystems.2014.09.00125218090http://www.journals.elsevier.com/biosystems/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:40:01Zoai:repositorium.sdum.uminho.pt:1822/31617Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:36:44.325197Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A kinetic model for curcumin production in Escherichia coli
title A kinetic model for curcumin production in Escherichia coli
spellingShingle A kinetic model for curcumin production in Escherichia coli
Machado, C. D.
Metabolic engineering
Kinetic modeling
Escherichia coli
Curcumin
Science & Technology
title_short A kinetic model for curcumin production in Escherichia coli
title_full A kinetic model for curcumin production in Escherichia coli
title_fullStr A kinetic model for curcumin production in Escherichia coli
title_full_unstemmed A kinetic model for curcumin production in Escherichia coli
title_sort A kinetic model for curcumin production in Escherichia coli
author Machado, C. D.
author_facet Machado, C. D.
Rodrigues, L. R.
Rocha, I.
author_role author
author2 Rodrigues, L. R.
Rocha, I.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Machado, C. D.
Rodrigues, L. R.
Rocha, I.
dc.subject.por.fl_str_mv Metabolic engineering
Kinetic modeling
Escherichia coli
Curcumin
Science & Technology
topic Metabolic engineering
Kinetic modeling
Escherichia coli
Curcumin
Science & Technology
description Curcumin is a natural compound obtained from turmeric, and is well known for its pharmacological effects. In this work, we design a heterologous pathway for industrial production of curcumin in Escherichia coli. A kinetic model of the pathway is then developed and connected to a kinetic model of the central carbon metabolism of E. coli. This model is used for optimization of the mutant strain through a rational design approach, and two manipulation targets are identified for overexpression. Dynamic simulations are then performed to compare the curcumin production profiles of the different mutant strains. Our results show that it is possible to obtain a significant improvement in the curcumin production rates with the proposed mutants. The kinetic model here developed can be an important framework to optimize curcumin production at an industrial scale and add value to its biomedical potential.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/31617
url http://hdl.handle.net/1822/31617
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0303-2647
10.1016/j.biosystems.2014.09.001
25218090
http://www.journals.elsevier.com/biosystems/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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