Genetic diversity assessment of portuguese cultivated Vicia faba L. through IRAP markers

Detalhes bibliográficos
Autor(a) principal: Tomás, Diana
Data de Publicação: 2016
Outros Autores: Dias, Ana Luísa, Silva, Manuela, Oliveira, Hugo R., Suso, Maria José, Viegas, Wanda, Veloso, Maria Manuela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/11314
Resumo: Faba bean have been grown in Portugal for a long time and locally adapted populations are still maintained on farm. The genetic diversity of four Portuguese faba bean populations that are still cultivated in some regions of the country was evaluated using the Inter Retrotransposons Amplified Polymorphism (IRAP) technique. It was shown that molecular markers based on retrotransposons previously identified in other species can be efficiently used in the genetic variability assessment of Vicia faba. The IRAP experiment targeting Athila yielded the most informative banding patterns. Cluster analysis using the neighbor-joining algorithm generated a dendrogram that clearly shows the distribution pattern of V. faba samples. The four equina accessions are separated from each other and form two distinct clades while the two major faba bean accessions are not unequivocally separated by the IRAP. Fluorescent In Situ Hybridization (FISH) analysis of sequences amplified by IRAP Athila revealed a wide distribution throughout V. faba chromosomes, confirming the whole-genome coverage of this molecular marker. Morphological characteristics were also assessed through cluster analysis of seed characters using the unweighted pair group method arithmetic average (UPGMA) and principal component analysis (PCA), showing a clear discrimination between faba bean major and equina groups. It was also found that the seed character most relevant to distinguish accessions was 100 seed weight. Seed morphological traits and IRAP evaluation give similar results supporting the potential of IRAP analysis for genetic diversity studies
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spelling Genetic diversity assessment of portuguese cultivated Vicia faba L. through IRAP markersfaba bean locally adapted populationsgenomic diversityIRAPFaba bean have been grown in Portugal for a long time and locally adapted populations are still maintained on farm. The genetic diversity of four Portuguese faba bean populations that are still cultivated in some regions of the country was evaluated using the Inter Retrotransposons Amplified Polymorphism (IRAP) technique. It was shown that molecular markers based on retrotransposons previously identified in other species can be efficiently used in the genetic variability assessment of Vicia faba. The IRAP experiment targeting Athila yielded the most informative banding patterns. Cluster analysis using the neighbor-joining algorithm generated a dendrogram that clearly shows the distribution pattern of V. faba samples. The four equina accessions are separated from each other and form two distinct clades while the two major faba bean accessions are not unequivocally separated by the IRAP. Fluorescent In Situ Hybridization (FISH) analysis of sequences amplified by IRAP Athila revealed a wide distribution throughout V. faba chromosomes, confirming the whole-genome coverage of this molecular marker. Morphological characteristics were also assessed through cluster analysis of seed characters using the unweighted pair group method arithmetic average (UPGMA) and principal component analysis (PCA), showing a clear discrimination between faba bean major and equina groups. It was also found that the seed character most relevant to distinguish accessions was 100 seed weight. Seed morphological traits and IRAP evaluation give similar results supporting the potential of IRAP analysis for genetic diversity studiesMDPIRepositório da Universidade de LisboaTomás, DianaDias, Ana LuísaSilva, ManuelaOliveira, Hugo R.Suso, Maria JoséViegas, WandaVeloso, Maria Manuela2016-04-04T13:13:34Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/11314eng"Diversity". ISSN 1424-2818. 8 (8) (2016) 11 p.10.3390/id8020008info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:41:36Zoai:www.repository.utl.pt:10400.5/11314Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:57:36.837291Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genetic diversity assessment of portuguese cultivated Vicia faba L. through IRAP markers
title Genetic diversity assessment of portuguese cultivated Vicia faba L. through IRAP markers
spellingShingle Genetic diversity assessment of portuguese cultivated Vicia faba L. through IRAP markers
Tomás, Diana
faba bean locally adapted populations
genomic diversity
IRAP
title_short Genetic diversity assessment of portuguese cultivated Vicia faba L. through IRAP markers
title_full Genetic diversity assessment of portuguese cultivated Vicia faba L. through IRAP markers
title_fullStr Genetic diversity assessment of portuguese cultivated Vicia faba L. through IRAP markers
title_full_unstemmed Genetic diversity assessment of portuguese cultivated Vicia faba L. through IRAP markers
title_sort Genetic diversity assessment of portuguese cultivated Vicia faba L. through IRAP markers
author Tomás, Diana
author_facet Tomás, Diana
Dias, Ana Luísa
Silva, Manuela
Oliveira, Hugo R.
Suso, Maria José
Viegas, Wanda
Veloso, Maria Manuela
author_role author
author2 Dias, Ana Luísa
Silva, Manuela
Oliveira, Hugo R.
Suso, Maria José
Viegas, Wanda
Veloso, Maria Manuela
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Tomás, Diana
Dias, Ana Luísa
Silva, Manuela
Oliveira, Hugo R.
Suso, Maria José
Viegas, Wanda
Veloso, Maria Manuela
dc.subject.por.fl_str_mv faba bean locally adapted populations
genomic diversity
IRAP
topic faba bean locally adapted populations
genomic diversity
IRAP
description Faba bean have been grown in Portugal for a long time and locally adapted populations are still maintained on farm. The genetic diversity of four Portuguese faba bean populations that are still cultivated in some regions of the country was evaluated using the Inter Retrotransposons Amplified Polymorphism (IRAP) technique. It was shown that molecular markers based on retrotransposons previously identified in other species can be efficiently used in the genetic variability assessment of Vicia faba. The IRAP experiment targeting Athila yielded the most informative banding patterns. Cluster analysis using the neighbor-joining algorithm generated a dendrogram that clearly shows the distribution pattern of V. faba samples. The four equina accessions are separated from each other and form two distinct clades while the two major faba bean accessions are not unequivocally separated by the IRAP. Fluorescent In Situ Hybridization (FISH) analysis of sequences amplified by IRAP Athila revealed a wide distribution throughout V. faba chromosomes, confirming the whole-genome coverage of this molecular marker. Morphological characteristics were also assessed through cluster analysis of seed characters using the unweighted pair group method arithmetic average (UPGMA) and principal component analysis (PCA), showing a clear discrimination between faba bean major and equina groups. It was also found that the seed character most relevant to distinguish accessions was 100 seed weight. Seed morphological traits and IRAP evaluation give similar results supporting the potential of IRAP analysis for genetic diversity studies
publishDate 2016
dc.date.none.fl_str_mv 2016-04-04T13:13:34Z
2016
2016-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/11314
url http://hdl.handle.net/10400.5/11314
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Diversity". ISSN 1424-2818. 8 (8) (2016) 11 p.
10.3390/id8020008
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
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dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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