Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain

Detalhes bibliográficos
Autor(a) principal: Santos, Susana Isabel Duarte dos
Data de Publicação: 2019
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/130114
Resumo: "Staphylococcus epidermidis is a Gram-positive commensal bacterium found mainly in mucous membranes but also in sebaceous areas. It is considered an opportunistic pathogen associated with nosocomial infections. Indeed, S. epidermidis is the main cause of coagulase negative staphylococci infections associated with medical devices. This thesis aims to understand the impact of pH conditions which mimic the skin pH (5.5) and blood pH (7.4) in a S. epidermidis commensal strain (19N) biological processes and metabolic pathways. Proteomic studies were realized by nanoscale liquid chromatography-tandem mass spectrometry (nano LC-MS/MS) and metabolomic studies through nuclear magnetic resonance (NMR) in the whole cellular extract."
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spelling Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strainStaphylococcus epidermidisinfection processproteomicmetabolomic"Staphylococcus epidermidis is a Gram-positive commensal bacterium found mainly in mucous membranes but also in sebaceous areas. It is considered an opportunistic pathogen associated with nosocomial infections. Indeed, S. epidermidis is the main cause of coagulase negative staphylococci infections associated with medical devices. This thesis aims to understand the impact of pH conditions which mimic the skin pH (5.5) and blood pH (7.4) in a S. epidermidis commensal strain (19N) biological processes and metabolic pathways. Proteomic studies were realized by nanoscale liquid chromatography-tandem mass spectrometry (nano LC-MS/MS) and metabolomic studies through nuclear magnetic resonance (NMR) in the whole cellular extract."Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António XavierRUNSantos, Susana Isabel Duarte dos2022-11-30T01:34:03Z2019-11-292019-11-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/130114enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:08:53Zoai:run.unl.pt:10362/130114Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:46:41.782475Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain
title Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain
spellingShingle Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain
Santos, Susana Isabel Duarte dos
Staphylococcus epidermidis
infection process
proteomic
metabolomic
title_short Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain
title_full Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain
title_fullStr Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain
title_full_unstemmed Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain
title_sort Characterization of biological and metabolic responses to pH changes in a commensal Staphylococcus epidermidis strain
author Santos, Susana Isabel Duarte dos
author_facet Santos, Susana Isabel Duarte dos
author_role author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Santos, Susana Isabel Duarte dos
dc.subject.por.fl_str_mv Staphylococcus epidermidis
infection process
proteomic
metabolomic
topic Staphylococcus epidermidis
infection process
proteomic
metabolomic
description "Staphylococcus epidermidis is a Gram-positive commensal bacterium found mainly in mucous membranes but also in sebaceous areas. It is considered an opportunistic pathogen associated with nosocomial infections. Indeed, S. epidermidis is the main cause of coagulase negative staphylococci infections associated with medical devices. This thesis aims to understand the impact of pH conditions which mimic the skin pH (5.5) and blood pH (7.4) in a S. epidermidis commensal strain (19N) biological processes and metabolic pathways. Proteomic studies were realized by nanoscale liquid chromatography-tandem mass spectrometry (nano LC-MS/MS) and metabolomic studies through nuclear magnetic resonance (NMR) in the whole cellular extract."
publishDate 2019
dc.date.none.fl_str_mv 2019-11-29
2019-11-29T00:00:00Z
2022-11-30T01:34:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/130114
url http://hdl.handle.net/10362/130114
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier
publisher.none.fl_str_mv Universidade Nova de Lisboa. Instituto de Tecnologia Química e Biológica António Xavier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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