Matrix gla protein in xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations
Autor(a) principal: | |
---|---|
Data de Publicação: | 2001 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/11930 |
Resumo: | Matrix Gla protein (MGP) belongs to the family of vitamin K-dependent, Gla-containing proteins and in higher vertebrates, is found in the extracellular matrix of mineralized tissues and soft tissues. MGP synthesis is highly regulated at the transcription and posttranscription levels and is now known to be involved in the regulation of extracellular matrix calcification and maintenance of cartilage and soft tissue integrity during growth and development. However, its mode of action at the molecular level remains unknown. Because there is a large degree of conservation between amino,acid sequences of shark and human MGP, the function of MGP probably has been conserved throughout evolution. Given the complexity of the mammalian system, the study of MGP in a lower vertebrate might be advantageous to relate the onset of MGP expression with specific events during development. Toward this goal, MGP was purified from Xenopus long bones and its N-terminal amino acid sequence was determined and used to clone the Xenopus MGP complementary DNA (cDNA) by a mixture of reverse-transcription (RT)- and 5'- rapid amplification of cDNA ends (RACE)-polymerase chain reaction (PCR). MGP messenger RNA (mRNA) was present in all tissues analyzed although predominantly expressed in Xenopus bone and heart and its presence was detected early in development at the onset of chondrocranium development and long before the appearance of the first calcified structures and metamorphosis. These results show that in this system, as in mammals, MGP may be required to delay or prevent mineralization of cartilage and soft tissues during the early stages of development and indicate that Xenopus is an adequate model organism to further study MGP function during growth and development. |
id |
RCAP_38f23f148d77dbb010f7f5e37995d439 |
---|---|
oai_identifier_str |
oai:sapientia.ualg.pt:10400.1/11930 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Matrix gla protein in xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerationsCarboxyglutamic acid proteinSubstrate recognitionMessenger-rnaBoneRatSequenceExpressionGeneIdentificationCartilageMatrix Gla proteinXenopus laeviscDNAEvolutionDevelopmentMatrix Gla protein (MGP) belongs to the family of vitamin K-dependent, Gla-containing proteins and in higher vertebrates, is found in the extracellular matrix of mineralized tissues and soft tissues. MGP synthesis is highly regulated at the transcription and posttranscription levels and is now known to be involved in the regulation of extracellular matrix calcification and maintenance of cartilage and soft tissue integrity during growth and development. However, its mode of action at the molecular level remains unknown. Because there is a large degree of conservation between amino,acid sequences of shark and human MGP, the function of MGP probably has been conserved throughout evolution. Given the complexity of the mammalian system, the study of MGP in a lower vertebrate might be advantageous to relate the onset of MGP expression with specific events during development. Toward this goal, MGP was purified from Xenopus long bones and its N-terminal amino acid sequence was determined and used to clone the Xenopus MGP complementary DNA (cDNA) by a mixture of reverse-transcription (RT)- and 5'- rapid amplification of cDNA ends (RACE)-polymerase chain reaction (PCR). MGP messenger RNA (mRNA) was present in all tissues analyzed although predominantly expressed in Xenopus bone and heart and its presence was detected early in development at the onset of chondrocranium development and long before the appearance of the first calcified structures and metamorphosis. These results show that in this system, as in mammals, MGP may be required to delay or prevent mineralization of cartilage and soft tissues during the early stages of development and indicate that Xenopus is an adequate model organism to further study MGP function during growth and development.NATO/CRG940751/SA5.2.05, Praxis XXI/BIA 469/94, (NIH; grant AR 25921) (Praxis XXI/BPD/18816) (Praxis XXI/BICJ-2985)WileySapientiaCancela, M. LeonorOhresser, M. C. P.Reia, J. P.S B Viegas, CarlaWilliamson, M. K.Price, P. A.2018-12-07T14:58:15Z2001-092001-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/11930eng0884-043110.1359/jbmr.2001.16.9.1611info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:23:50Zoai:sapientia.ualg.pt:10400.1/11930Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:03:21.966444Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Matrix gla protein in xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations |
title |
Matrix gla protein in xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations |
spellingShingle |
Matrix gla protein in xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations Cancela, M. Leonor Carboxyglutamic acid protein Substrate recognition Messenger-rna Bone Rat Sequence Expression Gene Identification Cartilage Matrix Gla protein Xenopus laevis cDNA Evolution Development |
title_short |
Matrix gla protein in xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations |
title_full |
Matrix gla protein in xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations |
title_fullStr |
Matrix gla protein in xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations |
title_full_unstemmed |
Matrix gla protein in xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations |
title_sort |
Matrix gla protein in xenopus laevis: molecular cloning, tissue distribution, and evolutionary considerations |
author |
Cancela, M. Leonor |
author_facet |
Cancela, M. Leonor Ohresser, M. C. P. Reia, J. P. S B Viegas, Carla Williamson, M. K. Price, P. A. |
author_role |
author |
author2 |
Ohresser, M. C. P. Reia, J. P. S B Viegas, Carla Williamson, M. K. Price, P. A. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Cancela, M. Leonor Ohresser, M. C. P. Reia, J. P. S B Viegas, Carla Williamson, M. K. Price, P. A. |
dc.subject.por.fl_str_mv |
Carboxyglutamic acid protein Substrate recognition Messenger-rna Bone Rat Sequence Expression Gene Identification Cartilage Matrix Gla protein Xenopus laevis cDNA Evolution Development |
topic |
Carboxyglutamic acid protein Substrate recognition Messenger-rna Bone Rat Sequence Expression Gene Identification Cartilage Matrix Gla protein Xenopus laevis cDNA Evolution Development |
description |
Matrix Gla protein (MGP) belongs to the family of vitamin K-dependent, Gla-containing proteins and in higher vertebrates, is found in the extracellular matrix of mineralized tissues and soft tissues. MGP synthesis is highly regulated at the transcription and posttranscription levels and is now known to be involved in the regulation of extracellular matrix calcification and maintenance of cartilage and soft tissue integrity during growth and development. However, its mode of action at the molecular level remains unknown. Because there is a large degree of conservation between amino,acid sequences of shark and human MGP, the function of MGP probably has been conserved throughout evolution. Given the complexity of the mammalian system, the study of MGP in a lower vertebrate might be advantageous to relate the onset of MGP expression with specific events during development. Toward this goal, MGP was purified from Xenopus long bones and its N-terminal amino acid sequence was determined and used to clone the Xenopus MGP complementary DNA (cDNA) by a mixture of reverse-transcription (RT)- and 5'- rapid amplification of cDNA ends (RACE)-polymerase chain reaction (PCR). MGP messenger RNA (mRNA) was present in all tissues analyzed although predominantly expressed in Xenopus bone and heart and its presence was detected early in development at the onset of chondrocranium development and long before the appearance of the first calcified structures and metamorphosis. These results show that in this system, as in mammals, MGP may be required to delay or prevent mineralization of cartilage and soft tissues during the early stages of development and indicate that Xenopus is an adequate model organism to further study MGP function during growth and development. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-09 2001-09-01T00:00:00Z 2018-12-07T14:58:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/11930 |
url |
http://hdl.handle.net/10400.1/11930 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0884-0431 10.1359/jbmr.2001.16.9.1611 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133267973111808 |