Norovirus Genetic Diversity in Children under Five Years Old with Acute Diarrhea in Mozambique (2014–2015)
| Autor(a) principal: | |
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| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/147865 |
Resumo: | Funding Information: This study was supported by the European Initiative for Research in Neglected Tropical Diseases (EFINTD), World Health Organization (WHO), The Global Alliance for Vaccines and Immunizations—Health System Strengthening (GAVI—HSS), Fundo Nacional de Investigação (FNI) and the National Research Foundation, South Africa (M.B.T., Grant specific reference number (UID 85799). Any opinion, finding, conclusion or recommendation expressed in this material is that of the author(s), and the NRF does not accept any liability in this regard. Publisher Copyright: © 2022 by the authors. |
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Norovirus Genetic Diversity in Children under Five Years Old with Acute Diarrhea in Mozambique (2014–2015)acute diarrheachildrengenotypesMozambiquenorovirusRJ101 Child Health. Child health servicesQR355 VirologyInfectious DiseasesVirologyMedical Laboratory TechnologyPediatrics, Perinatology, and Child HealthSDG 3 - Good Health and Well-beingSDG 9 - Industry, Innovation, and InfrastructureFunding Information: This study was supported by the European Initiative for Research in Neglected Tropical Diseases (EFINTD), World Health Organization (WHO), The Global Alliance for Vaccines and Immunizations—Health System Strengthening (GAVI—HSS), Fundo Nacional de Investigação (FNI) and the National Research Foundation, South Africa (M.B.T., Grant specific reference number (UID 85799). Any opinion, finding, conclusion or recommendation expressed in this material is that of the author(s), and the NRF does not accept any liability in this regard. Publisher Copyright: © 2022 by the authors.Norovirus (NoV) is the second most important cause of viral diarrheal disease in children worldwide after rotavirus and is estimated to be responsible for 17% of acute diarrhea in low-income countries. This study aimed to identify and report NoV genotypes in Mozambican children under the age of five years with acute diarrhea. Between May 2014 and December 2015, stool specimens were collected within the Mozambique Diarrhea National Surveillance (ViNaDia) and tested for NoV genogroups I (GI) and II (GII) using conventional reverse transcriptase-polymerase chain reaction (RT-PCR). Partial capsid and RNA-dependent RNA polymerase (RdRp) nucleotide sequences were aligned using the Muscle tool, and phylogenetic analyses were performed using MEGA X. A total of 204 stool specimens were tested for NoV. The detection rate of NoV was 14.2% (29/204). The presence of NoV was confirmed, by real-time RT-PCR (RT-qPCR), in 24/29 (82.8%) specimens, and NoV GII predominated (70.8%; 17/24). NoV GII.4 Sydney 2012[P31] was the predominant genotype/P-type combination detected (30.4%; 7/23). This is the first study which highlights the high genetic diversity of NoV in Mozambican children and the need to establish a continuous NoV surveillance system.Instituto de Higiene e Medicina Tropical (IHMT)RUNChilaúle, Jorfélia J.Munlela, BenildeMans, JanetMabasa, Victor V.Marques, SelmaBauhofer, Adilson Fernando LoforteJane, GrazielaAnapakala, EldaOliveira, FernandaCossa-Moiane, IdaléciaGuimarães, EsperançaSambo, JúliaBero, Diocreciano MatiasChissaque, AssucênioDeus, Nilsa deTaylor, Maureen B.2023-01-19T22:19:24Z2022-092022-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/147865eng1999-4915PURE: 50808802https://doi.org/10.3390/v14092001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-10T16:11:37ZPortal AgregadorONG |
| dc.title.none.fl_str_mv |
Norovirus Genetic Diversity in Children under Five Years Old with Acute Diarrhea in Mozambique (2014–2015) |
| title |
Norovirus Genetic Diversity in Children under Five Years Old with Acute Diarrhea in Mozambique (2014–2015) |
| spellingShingle |
Norovirus Genetic Diversity in Children under Five Years Old with Acute Diarrhea in Mozambique (2014–2015) Chilaúle, Jorfélia J. acute diarrhea children genotypes Mozambique norovirus RJ101 Child Health. Child health services QR355 Virology Infectious Diseases Virology Medical Laboratory Technology Pediatrics, Perinatology, and Child Health SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure |
| title_short |
Norovirus Genetic Diversity in Children under Five Years Old with Acute Diarrhea in Mozambique (2014–2015) |
| title_full |
Norovirus Genetic Diversity in Children under Five Years Old with Acute Diarrhea in Mozambique (2014–2015) |
| title_fullStr |
Norovirus Genetic Diversity in Children under Five Years Old with Acute Diarrhea in Mozambique (2014–2015) |
| title_full_unstemmed |
Norovirus Genetic Diversity in Children under Five Years Old with Acute Diarrhea in Mozambique (2014–2015) |
| title_sort |
Norovirus Genetic Diversity in Children under Five Years Old with Acute Diarrhea in Mozambique (2014–2015) |
| author |
Chilaúle, Jorfélia J. |
| author_facet |
Chilaúle, Jorfélia J. Munlela, Benilde Mans, Janet Mabasa, Victor V. Marques, Selma Bauhofer, Adilson Fernando Loforte Jane, Graziela Anapakala, Elda Oliveira, Fernanda Cossa-Moiane, Idalécia Guimarães, Esperança Sambo, Júlia Bero, Diocreciano Matias Chissaque, Assucênio Deus, Nilsa de Taylor, Maureen B. |
| author_role |
author |
| author2 |
Munlela, Benilde Mans, Janet Mabasa, Victor V. Marques, Selma Bauhofer, Adilson Fernando Loforte Jane, Graziela Anapakala, Elda Oliveira, Fernanda Cossa-Moiane, Idalécia Guimarães, Esperança Sambo, Júlia Bero, Diocreciano Matias Chissaque, Assucênio Deus, Nilsa de Taylor, Maureen B. |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) RUN |
| dc.contributor.author.fl_str_mv |
Chilaúle, Jorfélia J. Munlela, Benilde Mans, Janet Mabasa, Victor V. Marques, Selma Bauhofer, Adilson Fernando Loforte Jane, Graziela Anapakala, Elda Oliveira, Fernanda Cossa-Moiane, Idalécia Guimarães, Esperança Sambo, Júlia Bero, Diocreciano Matias Chissaque, Assucênio Deus, Nilsa de Taylor, Maureen B. |
| dc.subject.por.fl_str_mv |
acute diarrhea children genotypes Mozambique norovirus RJ101 Child Health. Child health services QR355 Virology Infectious Diseases Virology Medical Laboratory Technology Pediatrics, Perinatology, and Child Health SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure |
| topic |
acute diarrhea children genotypes Mozambique norovirus RJ101 Child Health. Child health services QR355 Virology Infectious Diseases Virology Medical Laboratory Technology Pediatrics, Perinatology, and Child Health SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure |
| description |
Funding Information: This study was supported by the European Initiative for Research in Neglected Tropical Diseases (EFINTD), World Health Organization (WHO), The Global Alliance for Vaccines and Immunizations—Health System Strengthening (GAVI—HSS), Fundo Nacional de Investigação (FNI) and the National Research Foundation, South Africa (M.B.T., Grant specific reference number (UID 85799). Any opinion, finding, conclusion or recommendation expressed in this material is that of the author(s), and the NRF does not accept any liability in this regard. Publisher Copyright: © 2022 by the authors. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-09 2022-09-01T00:00:00Z 2023-01-19T22:19:24Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/147865 |
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http://hdl.handle.net/10362/147865 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1999-4915 PURE: 50808802 https://doi.org/10.3390/v14092001 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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