Antimicrobial Resistance and Biofilms Underlying Catheter-Related Bloodstream Coinfection by Enterobacter cloacae Complex and Candida parapsilosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10400.18/8311 |
Resumo: | This article belongs to the Special Issue Microbial Biofilms, Antimicrobials, and Virulence Determinants. |
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Antimicrobial Resistance and Biofilms Underlying Catheter-Related Bloodstream Coinfection by Enterobacter cloacae Complex and Candida parapsilosisBiofilmsCatheter-related Bloodstream InfectionsPolymicrobial BiofilmsAntimicrobial ResistanceMicroscopyWhole Genome SequencingResistência aos AntimicrobianosInfecções Sistémicas e ZoonosesThis article belongs to the Special Issue Microbial Biofilms, Antimicrobials, and Virulence Determinants.Biofilm-associated infections are a public health concern especially in the context of healthcare-associated infections such as catheter-related bloodstream infections (CRBSIs). We evaluated the biofilm formation and antimicrobials resistance (AMR) of Enterobacter cloacae complex and Candida parapsilosis co-isolated from a CRBSI patient. Antimicrobial susceptibility of central venous catheters (CVCs) and hemoculture (HC) isolates was evaluated, including whole genome sequencing (WGS) resistome analysis and evaluation of gene expression to obtain insight into their AMR determinants. Crystal violet assay was used to assess dual biofilm biomass and microscopy was used to elucidate a microorganism’s distribution within biofilms assembled on different materials. Bacteria were multidrug-resistant including resistance to colistin and beta-lactams, likely linked to the mcr-9-like phosphoethanolamine transferase and to an ACT family cephalosporin-hydrolyzing class C beta-lactamase, respectively. The R398I and Y132F mutations in the ERG11 gene and its differential expression might account for C. parapsilosis resistance to fluconazole. The phenotype of dual biofilms assembled on glass, polystyrene and polyurethane depends on the material and how biofilms were initiated by one or both pathogens. Biofilms assembled on polyurethane were denser and richer in the extracellular polymeric matrix, and microorganisms were differently distributed on the inner/outer surface of the CVC.This research was funded by Portuguese Fundação para a Ciência e a Tecnologia and by the Slovak Research and Development Agency under contracts of SK-PT-18-0006 as a part of the Bilateral Cooperation Program (2019–2022) and APVV-21-0302, and by the grant VEGA 1/0537/19 supported by the Ministry of Education, Science, Research and Sport of the Slovak Republic.MDPIRepositório Científico do Instituto Nacional de SaúdeŠtefánek, MatúšWenner, SigurdBorges, VítorPinto, MiguelGomes, João PauloRodrigues, JoãoFaria, IsabelPessanha, Maria AnaMartins, FilomenaSabino, RaquelVeríssimo, CristinaNogueira, Isabel D.Carvalho, Patrícia AlmeidaBujdáková, HelenaJordao, Luisa2022-11-15T12:31:51Z2022-09-142022-09-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8311engAntibiotics (Basel). 2022 Sep 14;11(9):1245. doi: 10.3390/antibiotics110912452079-638210.3390/antibiotics11091245info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:30Zoai:repositorio.insa.pt:10400.18/8311Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:57.878326Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Antimicrobial Resistance and Biofilms Underlying Catheter-Related Bloodstream Coinfection by Enterobacter cloacae Complex and Candida parapsilosis |
| title |
Antimicrobial Resistance and Biofilms Underlying Catheter-Related Bloodstream Coinfection by Enterobacter cloacae Complex and Candida parapsilosis |
| spellingShingle |
Antimicrobial Resistance and Biofilms Underlying Catheter-Related Bloodstream Coinfection by Enterobacter cloacae Complex and Candida parapsilosis Štefánek, Matúš Biofilms Catheter-related Bloodstream Infections Polymicrobial Biofilms Antimicrobial Resistance Microscopy Whole Genome Sequencing Resistência aos Antimicrobianos Infecções Sistémicas e Zoonoses |
| title_short |
Antimicrobial Resistance and Biofilms Underlying Catheter-Related Bloodstream Coinfection by Enterobacter cloacae Complex and Candida parapsilosis |
| title_full |
Antimicrobial Resistance and Biofilms Underlying Catheter-Related Bloodstream Coinfection by Enterobacter cloacae Complex and Candida parapsilosis |
| title_fullStr |
Antimicrobial Resistance and Biofilms Underlying Catheter-Related Bloodstream Coinfection by Enterobacter cloacae Complex and Candida parapsilosis |
| title_full_unstemmed |
Antimicrobial Resistance and Biofilms Underlying Catheter-Related Bloodstream Coinfection by Enterobacter cloacae Complex and Candida parapsilosis |
| title_sort |
Antimicrobial Resistance and Biofilms Underlying Catheter-Related Bloodstream Coinfection by Enterobacter cloacae Complex and Candida parapsilosis |
| author |
Štefánek, Matúš |
| author_facet |
Štefánek, Matúš Wenner, Sigurd Borges, Vítor Pinto, Miguel Gomes, João Paulo Rodrigues, João Faria, Isabel Pessanha, Maria Ana Martins, Filomena Sabino, Raquel Veríssimo, Cristina Nogueira, Isabel D. Carvalho, Patrícia Almeida Bujdáková, Helena Jordao, Luisa |
| author_role |
author |
| author2 |
Wenner, Sigurd Borges, Vítor Pinto, Miguel Gomes, João Paulo Rodrigues, João Faria, Isabel Pessanha, Maria Ana Martins, Filomena Sabino, Raquel Veríssimo, Cristina Nogueira, Isabel D. Carvalho, Patrícia Almeida Bujdáková, Helena Jordao, Luisa |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
| dc.contributor.author.fl_str_mv |
Štefánek, Matúš Wenner, Sigurd Borges, Vítor Pinto, Miguel Gomes, João Paulo Rodrigues, João Faria, Isabel Pessanha, Maria Ana Martins, Filomena Sabino, Raquel Veríssimo, Cristina Nogueira, Isabel D. Carvalho, Patrícia Almeida Bujdáková, Helena Jordao, Luisa |
| dc.subject.por.fl_str_mv |
Biofilms Catheter-related Bloodstream Infections Polymicrobial Biofilms Antimicrobial Resistance Microscopy Whole Genome Sequencing Resistência aos Antimicrobianos Infecções Sistémicas e Zoonoses |
| topic |
Biofilms Catheter-related Bloodstream Infections Polymicrobial Biofilms Antimicrobial Resistance Microscopy Whole Genome Sequencing Resistência aos Antimicrobianos Infecções Sistémicas e Zoonoses |
| description |
This article belongs to the Special Issue Microbial Biofilms, Antimicrobials, and Virulence Determinants. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-11-15T12:31:51Z 2022-09-14 2022-09-14T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/8311 |
| url |
http://hdl.handle.net/10400.18/8311 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Antibiotics (Basel). 2022 Sep 14;11(9):1245. doi: 10.3390/antibiotics11091245 2079-6382 10.3390/antibiotics11091245 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
MDPI |
| publisher.none.fl_str_mv |
MDPI |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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