Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10174/19608 |
Resumo: | The repertoires of bitter-taste receptor (T2R) gene have been described for several animal species, but these data are still scarce for Lagomorphs. The aim of the present work is to identify potential repertoires of T2R in several Lagomorph species, covering a wide geographical distribution. We studied these genes in Lepus timidus, L. europaeus, Oryctolagus cuniculus algirus, Romerolagus diazi, and Sylvilagus floridanus, using O. cuniculus cuniculus as control species for PCR and DNA sequencing. We studied the identities of the DNA sequences and built the corresponding phylogenetic tree. Sequencing was successful for both subspecies of O. cuniculus for all T2R genes studied, for five genes in Lepus, and for three genes in R. diazi and S. floridanus. We describe for the first time the partial repertoires of T2R genes for Lagomorphs species, other than the common rabbit. Our phylogenetic analyses indicate that sequence proximity levels follow the established taxonomic classification. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Identification of a bitter-taste receptor gene repertoire in different Lagomorphs speciesbitter-taste receptorsTAS2RLagomorphLepusOryctolagusRomerolagusSylvilagusThe repertoires of bitter-taste receptor (T2R) gene have been described for several animal species, but these data are still scarce for Lagomorphs. The aim of the present work is to identify potential repertoires of T2R in several Lagomorph species, covering a wide geographical distribution. We studied these genes in Lepus timidus, L. europaeus, Oryctolagus cuniculus algirus, Romerolagus diazi, and Sylvilagus floridanus, using O. cuniculus cuniculus as control species for PCR and DNA sequencing. We studied the identities of the DNA sequences and built the corresponding phylogenetic tree. Sequencing was successful for both subspecies of O. cuniculus for all T2R genes studied, for five genes in Lepus, and for three genes in R. diazi and S. floridanus. We describe for the first time the partial repertoires of T2R genes for Lagomorphs species, other than the common rabbit. Our phylogenetic analyses indicate that sequence proximity levels follow the established taxonomic classification.2017-01-09T12:42:22Z2017-01-092016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10174/19608http://hdl.handle.net/10174/19608enghttp://journal.frontiersin.org/article/10.3389/fgene.2016.00055/fullanammferreira@gmail.comndndndelsaba@uevora.ptndnddoi: 10.3389/fgene.2016.00055Ferreira, Ana M.Marques, Andreia TFontanesi, LucaThulin, Carl-GustafSales-Baptista, ElviraAraújo, Susana SAlmeida, André Minfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-08T04:22:43ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species |
title |
Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species |
spellingShingle |
Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species Ferreira, Ana M. bitter-taste receptors TAS2R Lagomorph Lepus Oryctolagus Romerolagus Sylvilagus |
title_short |
Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species |
title_full |
Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species |
title_fullStr |
Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species |
title_full_unstemmed |
Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species |
title_sort |
Identification of a bitter-taste receptor gene repertoire in different Lagomorphs species |
author |
Ferreira, Ana M. |
author_facet |
Ferreira, Ana M. Marques, Andreia T Fontanesi, Luca Thulin, Carl-Gustaf Sales-Baptista, Elvira Araújo, Susana S Almeida, André M |
author_role |
author |
author2 |
Marques, Andreia T Fontanesi, Luca Thulin, Carl-Gustaf Sales-Baptista, Elvira Araújo, Susana S Almeida, André M |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira, Ana M. Marques, Andreia T Fontanesi, Luca Thulin, Carl-Gustaf Sales-Baptista, Elvira Araújo, Susana S Almeida, André M |
dc.subject.por.fl_str_mv |
bitter-taste receptors TAS2R Lagomorph Lepus Oryctolagus Romerolagus Sylvilagus |
topic |
bitter-taste receptors TAS2R Lagomorph Lepus Oryctolagus Romerolagus Sylvilagus |
description |
The repertoires of bitter-taste receptor (T2R) gene have been described for several animal species, but these data are still scarce for Lagomorphs. The aim of the present work is to identify potential repertoires of T2R in several Lagomorph species, covering a wide geographical distribution. We studied these genes in Lepus timidus, L. europaeus, Oryctolagus cuniculus algirus, Romerolagus diazi, and Sylvilagus floridanus, using O. cuniculus cuniculus as control species for PCR and DNA sequencing. We studied the identities of the DNA sequences and built the corresponding phylogenetic tree. Sequencing was successful for both subspecies of O. cuniculus for all T2R genes studied, for five genes in Lepus, and for three genes in R. diazi and S. floridanus. We describe for the first time the partial repertoires of T2R genes for Lagomorphs species, other than the common rabbit. Our phylogenetic analyses indicate that sequence proximity levels follow the established taxonomic classification. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01T00:00:00Z 2017-01-09T12:42:22Z 2017-01-09 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10174/19608 http://hdl.handle.net/10174/19608 |
url |
http://hdl.handle.net/10174/19608 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://journal.frontiersin.org/article/10.3389/fgene.2016.00055/full anammferreira@gmail.com nd nd nd elsaba@uevora.pt nd nd doi: 10.3389/fgene.2016.00055 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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1777304624037888000 |