Nosocomial Outbreak of SARS-CoV-2 in a “Non-COVID-19” Hospital Ward: Virus Genome Sequencing as a Key Tool to Understand Cryptic Transmission

Detalhes bibliográficos
Autor(a) principal: Borges, Vítor
Data de Publicação: 2021
Outros Autores: Isidro, Joana, Macedo, Filipe, Neves, José, Silva, Luís, Paiva, Mário, Barata, José, Catarino, Judite, Ciobanu, Liliana, Duarte, Sílvia, Vieira, Luís, Guiomar, Raquel, Gomes, João Paulo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8054
Resumo: (This article belongs to the Collection SARS-CoV-2 and COVID-19)
id RCAP_39f578a7fe5bab570a65c523e2473d15
oai_identifier_str oai:repositorio.insa.pt:10400.18/8054
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Nosocomial Outbreak of SARS-CoV-2 in a “Non-COVID-19” Hospital Ward: Virus Genome Sequencing as a Key Tool to Understand Cryptic TransmissionCOVID-19SARS-CoV-2Contact TracingGenome SequencingHealthcare InstitutionNosocomial OutbreakInfecções Respiratórias(This article belongs to the Collection SARS-CoV-2 and COVID-19)Dissemination of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in healthcare institutions affects both patients and health-care workers (HCW), as well as the institutional capacity to provide essential health services. Here, we investigated an outbreak of SARS-CoV-2 in a "non-COVID-19" hospital ward unveiled by massive testing, which challenged the reconstruction of transmission chains. The contacts network during the 15-day period before the screening was investigated, and positive SARS-CoV-2 RNA samples were subjected to virus genome sequencing. Of the 245 tested individuals, 48 (21 patients and 27 HCWs) tested positive for SARS-CoV-2. HCWs were mostly asymptomatic, but the mortality among patients reached 57.1% (12/21). Phylogenetic reconstruction revealed that all cases were part of the same transmission chain. By combining contact tracing and genomic data, including analysis of emerging minor variants, we unveiled a scenario of silent SARS-CoV-2 dissemination, mostly driven by the close contact within the HCWs group and between HCWs and patients. This investigation triggered enhanced prevention and control measures, leading to more timely detection and containment of novel outbreaks. This study shows the benefit of combining genomic and epidemiological data for disclosing complex nosocomial outbreaks, and provides valuable data to prevent transmission of COVID-19 in healthcare facilities.This study is co-funded by Fundação para a Ciência e Tecnologia and Agência de Investigação Clínica e Inovação Biomédica (234_596874175) on behalf of the Research 4 COVID-19 call. This work is also a result of the GenomePT project (POCI-01-0145-FEDER-022184), supported by COMPETE 2020 - Operational Programme for Competitiveness and Internationalisation (POCI), Lisboa Portugal Regional Operational Programme (Lisboa2020), Algarve Portugal Regional Operational Programme (CRESC Algarve2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF), and by Fundação para a Ciência e a Tecnologia (FCT). This work was also supported by Fundos FEDER through the Programa Operacional Factores de Competitividade–COMPETE and by Fundos Nacionais through the Fundação para a Ciência e a Tecnologia within the scope of the project UID/BIM/00009/2019 (Centre for Toxicogenomics and Human Health -ToxOmics).MDPIRepositório Científico do Instituto Nacional de SaúdeBorges, VítorIsidro, JoanaMacedo, FilipeNeves, JoséSilva, LuísPaiva, MárioBarata, JoséCatarino, JuditeCiobanu, LilianaDuarte, SílviaVieira, LuísGuiomar, RaquelGomes, João Paulo2022-07-03T16:28:45Z2021-04-012021-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8054engViruses. 2021 Apr 1;13(4):604. doi: 10.3390/v13040604.1999-491510.3390/v13040604info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:25Zoai:repositorio.insa.pt:10400.18/8054Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:50.141044Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Nosocomial Outbreak of SARS-CoV-2 in a “Non-COVID-19” Hospital Ward: Virus Genome Sequencing as a Key Tool to Understand Cryptic Transmission
title Nosocomial Outbreak of SARS-CoV-2 in a “Non-COVID-19” Hospital Ward: Virus Genome Sequencing as a Key Tool to Understand Cryptic Transmission
spellingShingle Nosocomial Outbreak of SARS-CoV-2 in a “Non-COVID-19” Hospital Ward: Virus Genome Sequencing as a Key Tool to Understand Cryptic Transmission
Borges, Vítor
COVID-19
SARS-CoV-2
Contact Tracing
Genome Sequencing
Healthcare Institution
Nosocomial Outbreak
Infecções Respiratórias
title_short Nosocomial Outbreak of SARS-CoV-2 in a “Non-COVID-19” Hospital Ward: Virus Genome Sequencing as a Key Tool to Understand Cryptic Transmission
title_full Nosocomial Outbreak of SARS-CoV-2 in a “Non-COVID-19” Hospital Ward: Virus Genome Sequencing as a Key Tool to Understand Cryptic Transmission
title_fullStr Nosocomial Outbreak of SARS-CoV-2 in a “Non-COVID-19” Hospital Ward: Virus Genome Sequencing as a Key Tool to Understand Cryptic Transmission
title_full_unstemmed Nosocomial Outbreak of SARS-CoV-2 in a “Non-COVID-19” Hospital Ward: Virus Genome Sequencing as a Key Tool to Understand Cryptic Transmission
title_sort Nosocomial Outbreak of SARS-CoV-2 in a “Non-COVID-19” Hospital Ward: Virus Genome Sequencing as a Key Tool to Understand Cryptic Transmission
author Borges, Vítor
author_facet Borges, Vítor
Isidro, Joana
Macedo, Filipe
Neves, José
Silva, Luís
Paiva, Mário
Barata, José
Catarino, Judite
Ciobanu, Liliana
Duarte, Sílvia
Vieira, Luís
Guiomar, Raquel
Gomes, João Paulo
author_role author
author2 Isidro, Joana
Macedo, Filipe
Neves, José
Silva, Luís
Paiva, Mário
Barata, José
Catarino, Judite
Ciobanu, Liliana
Duarte, Sílvia
Vieira, Luís
Guiomar, Raquel
Gomes, João Paulo
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Borges, Vítor
Isidro, Joana
Macedo, Filipe
Neves, José
Silva, Luís
Paiva, Mário
Barata, José
Catarino, Judite
Ciobanu, Liliana
Duarte, Sílvia
Vieira, Luís
Guiomar, Raquel
Gomes, João Paulo
dc.subject.por.fl_str_mv COVID-19
SARS-CoV-2
Contact Tracing
Genome Sequencing
Healthcare Institution
Nosocomial Outbreak
Infecções Respiratórias
topic COVID-19
SARS-CoV-2
Contact Tracing
Genome Sequencing
Healthcare Institution
Nosocomial Outbreak
Infecções Respiratórias
description (This article belongs to the Collection SARS-CoV-2 and COVID-19)
publishDate 2021
dc.date.none.fl_str_mv 2021-04-01
2021-04-01T00:00:00Z
2022-07-03T16:28:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8054
url http://hdl.handle.net/10400.18/8054
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Viruses. 2021 Apr 1;13(4):604. doi: 10.3390/v13040604.
1999-4915
10.3390/v13040604
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132174685831168

Registros relacionados