Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.1/11669 |
Resumo: | Pantoea agglomerans CPA-2 is an effective biocontrol agent of postharvest diseases of citrus and pome fruit. A monitoring technique was developed for its identification and to quantify its populations. The methodology used consisted of (i) searching for a semi-selective medium, (ii) identification of molecular markers and (iii) monitoring population dynamics in a commercial trial. As a semi-selective medium, Malonate Broth Agar supplemented with tetracycline hydroxychloricle and incubation at high temperature (max. of 40 degrees C) facilitated the selective recovery of P agglomerans CPA-2 colonies. The RAPD technique was applied to a collection of 13 strains of P. agglomerans, including CPA-2. Among the 12 primers tested, OPL-11 amplified a fragment (about 720 bp) specific to strain CPA-2. On the basis of this fragment, two SCAR markers were amplified using a primer pair derived from OPL-11 elongation. A first SCAR marker of 720 bp was specifically amplified for the strain CPA-2 and a second one of 270bp was obtained for all P. agglomerans strains tested, including CPA-2. Commercial trials demonstrated a significant reduction of decay with the treatment of formulated cells of R agglomerans CPA-2. Population dynamics of CPA-2 in commercial trials were determined on fruit surfaces and in the environment using both the classical plating technique and PCR with SCAR primers. In general, no significant differences were observed between results obtained from the two methods. On fruit surfaces, 1 day after CPA-2 applied its population by classical methods was 4.37 x 10(6) cfu wound(-1) and at the end of the experiment the population increased to 5.8 x 10(5) cfu wound(-1). The percentages of colonies identified as P agglomerans CPA-2 at these sampling times using SCAR primers were 90 and 95%, respectively. Population dynamics in the environment to evaluate the environmental fate of R agglomerans CPA-2 showed that it has a limited persistence and limited capacity for dispersion. (c) 2007 Published by Elsevier B.V. |
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Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2Real-Time PcrBiological-Control AgentCandida-Sake Cpa-1Anomala Strain-KAureobasidium-PullulansApple FruitBlue MoldCombinationDiseasesViabilityPantoea agglomerans CPA-2 is an effective biocontrol agent of postharvest diseases of citrus and pome fruit. A monitoring technique was developed for its identification and to quantify its populations. The methodology used consisted of (i) searching for a semi-selective medium, (ii) identification of molecular markers and (iii) monitoring population dynamics in a commercial trial. As a semi-selective medium, Malonate Broth Agar supplemented with tetracycline hydroxychloricle and incubation at high temperature (max. of 40 degrees C) facilitated the selective recovery of P agglomerans CPA-2 colonies. The RAPD technique was applied to a collection of 13 strains of P. agglomerans, including CPA-2. Among the 12 primers tested, OPL-11 amplified a fragment (about 720 bp) specific to strain CPA-2. On the basis of this fragment, two SCAR markers were amplified using a primer pair derived from OPL-11 elongation. A first SCAR marker of 720 bp was specifically amplified for the strain CPA-2 and a second one of 270bp was obtained for all P. agglomerans strains tested, including CPA-2. Commercial trials demonstrated a significant reduction of decay with the treatment of formulated cells of R agglomerans CPA-2. Population dynamics of CPA-2 in commercial trials were determined on fruit surfaces and in the environment using both the classical plating technique and PCR with SCAR primers. In general, no significant differences were observed between results obtained from the two methods. On fruit surfaces, 1 day after CPA-2 applied its population by classical methods was 4.37 x 10(6) cfu wound(-1) and at the end of the experiment the population increased to 5.8 x 10(5) cfu wound(-1). The percentages of colonies identified as P agglomerans CPA-2 at these sampling times using SCAR primers were 90 and 95%, respectively. Population dynamics in the environment to evaluate the environmental fate of R agglomerans CPA-2 showed that it has a limited persistence and limited capacity for dispersion. (c) 2007 Published by Elsevier B.V.Elsevier Science BvSapientiaNunes, CarlaBajji, MohammedStepien, ValerieManso, TeresaTorres, RosarioUsall, JosepJijakli, M. Haissam2018-12-07T14:53:45Z2008-032008-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/11669eng0925-521410.1016/j.postharvbio.2007.07.016info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:23:31Zoai:sapientia.ualg.pt:10400.1/11669Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:03:08.636963Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2 |
title |
Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2 |
spellingShingle |
Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2 Nunes, Carla Real-Time Pcr Biological-Control Agent Candida-Sake Cpa-1 Anomala Strain-K Aureobasidium-Pullulans Apple Fruit Blue Mold Combination Diseases Viability |
title_short |
Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2 |
title_full |
Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2 |
title_fullStr |
Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2 |
title_full_unstemmed |
Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2 |
title_sort |
Development and application of a SCAR marker to monitor and quantify populations of the postharvest biocontrol agent Pantoea agglomerans CPA-2 |
author |
Nunes, Carla |
author_facet |
Nunes, Carla Bajji, Mohammed Stepien, Valerie Manso, Teresa Torres, Rosario Usall, Josep Jijakli, M. Haissam |
author_role |
author |
author2 |
Bajji, Mohammed Stepien, Valerie Manso, Teresa Torres, Rosario Usall, Josep Jijakli, M. Haissam |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Sapientia |
dc.contributor.author.fl_str_mv |
Nunes, Carla Bajji, Mohammed Stepien, Valerie Manso, Teresa Torres, Rosario Usall, Josep Jijakli, M. Haissam |
dc.subject.por.fl_str_mv |
Real-Time Pcr Biological-Control Agent Candida-Sake Cpa-1 Anomala Strain-K Aureobasidium-Pullulans Apple Fruit Blue Mold Combination Diseases Viability |
topic |
Real-Time Pcr Biological-Control Agent Candida-Sake Cpa-1 Anomala Strain-K Aureobasidium-Pullulans Apple Fruit Blue Mold Combination Diseases Viability |
description |
Pantoea agglomerans CPA-2 is an effective biocontrol agent of postharvest diseases of citrus and pome fruit. A monitoring technique was developed for its identification and to quantify its populations. The methodology used consisted of (i) searching for a semi-selective medium, (ii) identification of molecular markers and (iii) monitoring population dynamics in a commercial trial. As a semi-selective medium, Malonate Broth Agar supplemented with tetracycline hydroxychloricle and incubation at high temperature (max. of 40 degrees C) facilitated the selective recovery of P agglomerans CPA-2 colonies. The RAPD technique was applied to a collection of 13 strains of P. agglomerans, including CPA-2. Among the 12 primers tested, OPL-11 amplified a fragment (about 720 bp) specific to strain CPA-2. On the basis of this fragment, two SCAR markers were amplified using a primer pair derived from OPL-11 elongation. A first SCAR marker of 720 bp was specifically amplified for the strain CPA-2 and a second one of 270bp was obtained for all P. agglomerans strains tested, including CPA-2. Commercial trials demonstrated a significant reduction of decay with the treatment of formulated cells of R agglomerans CPA-2. Population dynamics of CPA-2 in commercial trials were determined on fruit surfaces and in the environment using both the classical plating technique and PCR with SCAR primers. In general, no significant differences were observed between results obtained from the two methods. On fruit surfaces, 1 day after CPA-2 applied its population by classical methods was 4.37 x 10(6) cfu wound(-1) and at the end of the experiment the population increased to 5.8 x 10(5) cfu wound(-1). The percentages of colonies identified as P agglomerans CPA-2 at these sampling times using SCAR primers were 90 and 95%, respectively. Population dynamics in the environment to evaluate the environmental fate of R agglomerans CPA-2 showed that it has a limited persistence and limited capacity for dispersion. (c) 2007 Published by Elsevier B.V. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-03 2008-03-01T00:00:00Z 2018-12-07T14:53:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.1/11669 |
url |
http://hdl.handle.net/10400.1/11669 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0925-5214 10.1016/j.postharvbio.2007.07.016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier Science Bv |
publisher.none.fl_str_mv |
Elsevier Science Bv |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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