Deficiency of Pkc1 activity affects glycerol metabolism in Saccharomyces cerevisiae

Detalhes bibliográficos
Autor(a) principal: Gomes, K. N.
Data de Publicação: 2005
Outros Autores: Freitas, S. M. A. C., Pais, T. M., Fietto, J. L. R., Totola, A. H., Arantes, R. M. E., Martins, A., Lucas, C., Schuller, Dorit Elisabeth, Casal, Margarida, Castro, I. M., Fietto, L. G., Brandão, R. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/2244
Resumo: In press
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spelling Deficiency of Pkc1 activity affects glycerol metabolism in Saccharomyces cerevisiaeProtein kinase CGlucose repressionGlycerol transportGlycerol metabolismScience & TechnologyIn pressProtein kinase C is apparently involved in the control of many cellular systems: the cell wall integrity pathway, the synthesis of ribosomes, the appropriated reallocation of transcription factors under specific stress conditions and also the regulation of N-glycosylation activity. All these observations suggest the existence of additional targets not yet identified. In the context of the control of carbon metabolism, previous data demonstrated that Pkc1 p might play a central role in the control of cellular growth and metabolism in yeast. In particular, it has been suggested that it might be involved in the derepression of genes under glucose-repression by driving an appropriated subcellular localization of transcriptional factors, such as Mig1 p. In this work, we show that pkc1∆ mutant is unable to grow on glycerol because it cannot perform the derepression of GUT1 gene that encodes for glycerol kinase. Additionally, active transport is also partially affected. Using this phenotype, we were able to isolate a new pkc1∆ revertant. We also isolated two transformants identified as the nuclear exportin Msn5 and the histone deacetylase Hos2 extragenic suppressors of this mutation. Based on these results, we postulate that Pkc1 p may be involved in the control of the cellular localization and/or regulation of the activity of nuclear proteins implicated in gene expression.Fundação Universidade Federal de Ouro Preto (FUFOP). Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG) - CBS-1875/95. Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) - 300998/89-9 to R.L.B., 301255/01-6 to L.G.F.Elsevier Science BVUniversidade do MinhoGomes, K. N.Freitas, S. M. A. C.Pais, T. M.Fietto, J. L. R.Totola, A. H.Arantes, R. M. E.Martins, A.Lucas, C.Schuller, Dorit ElisabethCasal, MargaridaCastro, I. M.Fietto, L. G.Brandão, R. L.2005-07-162005-07-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/2244eng"FEMS yeast research". ISSN 1567-1356. 5:8 (2005) 767-776.1567-135610.1016/j.femsyr.2005.01.00315851105http://www.elsevier.com/wps/find/homepage.cws_homeinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:52:40ZPortal AgregadorONG
dc.title.none.fl_str_mv Deficiency of Pkc1 activity affects glycerol metabolism in Saccharomyces cerevisiae
title Deficiency of Pkc1 activity affects glycerol metabolism in Saccharomyces cerevisiae
spellingShingle Deficiency of Pkc1 activity affects glycerol metabolism in Saccharomyces cerevisiae
Gomes, K. N.
Protein kinase C
Glucose repression
Glycerol transport
Glycerol metabolism
Science & Technology
title_short Deficiency of Pkc1 activity affects glycerol metabolism in Saccharomyces cerevisiae
title_full Deficiency of Pkc1 activity affects glycerol metabolism in Saccharomyces cerevisiae
title_fullStr Deficiency of Pkc1 activity affects glycerol metabolism in Saccharomyces cerevisiae
title_full_unstemmed Deficiency of Pkc1 activity affects glycerol metabolism in Saccharomyces cerevisiae
title_sort Deficiency of Pkc1 activity affects glycerol metabolism in Saccharomyces cerevisiae
author Gomes, K. N.
author_facet Gomes, K. N.
Freitas, S. M. A. C.
Pais, T. M.
Fietto, J. L. R.
Totola, A. H.
Arantes, R. M. E.
Martins, A.
Lucas, C.
Schuller, Dorit Elisabeth
Casal, Margarida
Castro, I. M.
Fietto, L. G.
Brandão, R. L.
author_role author
author2 Freitas, S. M. A. C.
Pais, T. M.
Fietto, J. L. R.
Totola, A. H.
Arantes, R. M. E.
Martins, A.
Lucas, C.
Schuller, Dorit Elisabeth
Casal, Margarida
Castro, I. M.
Fietto, L. G.
Brandão, R. L.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gomes, K. N.
Freitas, S. M. A. C.
Pais, T. M.
Fietto, J. L. R.
Totola, A. H.
Arantes, R. M. E.
Martins, A.
Lucas, C.
Schuller, Dorit Elisabeth
Casal, Margarida
Castro, I. M.
Fietto, L. G.
Brandão, R. L.
dc.subject.por.fl_str_mv Protein kinase C
Glucose repression
Glycerol transport
Glycerol metabolism
Science & Technology
topic Protein kinase C
Glucose repression
Glycerol transport
Glycerol metabolism
Science & Technology
description In press
publishDate 2005
dc.date.none.fl_str_mv 2005-07-16
2005-07-16T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/2244
url http://hdl.handle.net/1822/2244
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "FEMS yeast research". ISSN 1567-1356. 5:8 (2005) 767-776.
1567-1356
10.1016/j.femsyr.2005.01.003
15851105
http://www.elsevier.com/wps/find/homepage.cws_home
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Science BV
publisher.none.fl_str_mv Elsevier Science BV
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv
repository.mail.fl_str_mv
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