Evidence for the dissemination to humans of Methicillin-Resistant Staphylococcus aureus ST398 through the pork production chain : a study in a portuguese slaughterhouse

Detalhes bibliográficos
Autor(a) principal: Bouchami, Ons
Data de Publicação: 2020
Outros Autores: Fraqueza, M. J., Faria, Nuno Alexandre, Alves, Valquíria, Lawal, Opeyemi Uwangbaoje, Lencastre, Herminia de, Miragaia, Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/24117
Resumo: Research Areas: Microbiology
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spelling Evidence for the dissemination to humans of Methicillin-Resistant Staphylococcus aureus ST398 through the pork production chain : a study in a portuguese slaughterhouseMRSAST398SlaughterhousePork chain productionTransmissionInvasive diseaseResearch Areas: MicrobiologyLivestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) ST398 was recovered from infections in humans exposed to animals, raising public health concerns. However, contact with food producing chain as a means of transmission of LA-MRSA to humans remains poorly understood. We aimed to assess if pork production chain is a source of MRSA ST398 for human colonization and infection. MRSA from live pigs, meat, the environment, and slaughterhouse workers were analyzed by Pulsed-Field Gel Electrophoresis (PFGE), spa, MLST typing, SNPs and for antibiotic resistance and virulence gene profiles. We compared core and accessory genomes of MRSA ST398 isolated from slaughterhouse and hospital. We detected MRSA ST398 (t011, t108, t1451) along the entire pork production chain (live pigs: 60%; equipment: 38%; meat: 23%) and in workers (40%). All MRSA ST398 were multidrug resistant, and the majority carried genes encoding biocide resistance and enterotoxins. We found 23 cross-transmission events between live pigs, meat, and workers (6–55 SNPs). MRSA ST398 from infection and slaughterhouse environment belonged to the same clonal type (ST398, t011, SCCmec V), but differed in 321–378 SNPs. Pork production chain can be a source of MRSA ST398 for colonization of human slaughterhouse workers, which can represent a risk of subsequent meat contamination and human infection.MDPIRepositório da Universidade de LisboaBouchami, OnsFraqueza, M. J.Faria, Nuno AlexandreAlves, ValquíriaLawal, Opeyemi UwangbaojeLencastre, Herminia deMiragaia, Maria2022-04-19T22:47:01Z2020-11-292020-11-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/24117engBouchami O, Fraqueza MJ, Faria NA, Alves V, Lawal OU, Lencastre H, Miragaia M. 2020. Evidence for the dissemination to humans of Methicillin-Resistant Staphylococcus aureus ST398 through the pork production chain : a study in a portuguese slaughterhouse. Microorganisms, 8(12):1892. DOI 10.3390/microorganisms812189210.3390/microorganisms81218922076-2607info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:53:46Zoai:www.repository.utl.pt:10400.5/24117Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:08:14.213382Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Evidence for the dissemination to humans of Methicillin-Resistant Staphylococcus aureus ST398 through the pork production chain : a study in a portuguese slaughterhouse
title Evidence for the dissemination to humans of Methicillin-Resistant Staphylococcus aureus ST398 through the pork production chain : a study in a portuguese slaughterhouse
spellingShingle Evidence for the dissemination to humans of Methicillin-Resistant Staphylococcus aureus ST398 through the pork production chain : a study in a portuguese slaughterhouse
Bouchami, Ons
MRSA
ST398
Slaughterhouse
Pork chain production
Transmission
Invasive disease
title_short Evidence for the dissemination to humans of Methicillin-Resistant Staphylococcus aureus ST398 through the pork production chain : a study in a portuguese slaughterhouse
title_full Evidence for the dissemination to humans of Methicillin-Resistant Staphylococcus aureus ST398 through the pork production chain : a study in a portuguese slaughterhouse
title_fullStr Evidence for the dissemination to humans of Methicillin-Resistant Staphylococcus aureus ST398 through the pork production chain : a study in a portuguese slaughterhouse
title_full_unstemmed Evidence for the dissemination to humans of Methicillin-Resistant Staphylococcus aureus ST398 through the pork production chain : a study in a portuguese slaughterhouse
title_sort Evidence for the dissemination to humans of Methicillin-Resistant Staphylococcus aureus ST398 through the pork production chain : a study in a portuguese slaughterhouse
author Bouchami, Ons
author_facet Bouchami, Ons
Fraqueza, M. J.
Faria, Nuno Alexandre
Alves, Valquíria
Lawal, Opeyemi Uwangbaoje
Lencastre, Herminia de
Miragaia, Maria
author_role author
author2 Fraqueza, M. J.
Faria, Nuno Alexandre
Alves, Valquíria
Lawal, Opeyemi Uwangbaoje
Lencastre, Herminia de
Miragaia, Maria
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Bouchami, Ons
Fraqueza, M. J.
Faria, Nuno Alexandre
Alves, Valquíria
Lawal, Opeyemi Uwangbaoje
Lencastre, Herminia de
Miragaia, Maria
dc.subject.por.fl_str_mv MRSA
ST398
Slaughterhouse
Pork chain production
Transmission
Invasive disease
topic MRSA
ST398
Slaughterhouse
Pork chain production
Transmission
Invasive disease
description Research Areas: Microbiology
publishDate 2020
dc.date.none.fl_str_mv 2020-11-29
2020-11-29T00:00:00Z
2022-04-19T22:47:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/24117
url http://hdl.handle.net/10400.5/24117
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bouchami O, Fraqueza MJ, Faria NA, Alves V, Lawal OU, Lencastre H, Miragaia M. 2020. Evidence for the dissemination to humans of Methicillin-Resistant Staphylococcus aureus ST398 through the pork production chain : a study in a portuguese slaughterhouse. Microorganisms, 8(12):1892. DOI 10.3390/microorganisms8121892
10.3390/microorganisms8121892
2076-2607
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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