Spin-coated polysaccharide-based multilayered freestanding films with adhesive and bioactive moieties

Detalhes bibliográficos
Autor(a) principal: Moreira, Joana
Data de Publicação: 2020
Outros Autores: Vale, A. Catarina, Pires, R. A., Botelho, Gabriela, Reis, R. L., Alves, N. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/64578
Resumo: Freestanding films based on catechol functionalized chitosan (CHI), hyaluronic acid (HA), and bioglass nanoparticles (BGNPs) were developed by spin-coating layer-by-layer assembly (SA-LbL). The catechol groups of 3,4-dihydroxy-<span style="font-variant: small-caps;">l</span>-phenylalanine (DOPA) present in the marine mussels adhesive proteins (MAPs) are the main factors responsible for their characteristic strong wet adhesion. Then, the produced films were cross-linked with genipin to improve their stability in wet state. Overall, the incorporation of BGNPs resulted in thicker and bioactive films, hydrophilic and rougher surfaces, reduced swelling, higher weight loss, and lower stiffness. The incorporation of catechol groups onto the films showed a significant increase in the films’ adhesion and stiffness, lower swelling, and weight loss. Interestingly, a synergetic effect on the stiffness increase was observed upon the combined incorporation of BGNPs with catechol-modified polymers, given that such films were the stiffest. Regarding the biological assays, the films exhibited no negative effects on cellular viability, adhesion, and proliferation, and the BGNPs seemed to promote higher cellular metabolic activity. These bioactive LbL freestanding films combine enhanced adhesion with improved mechanical properties and could find applications in the biomedical field, such as guided hard tissue regeneration membranes.
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spelling Spin-coated polysaccharide-based multilayered freestanding films with adhesive and bioactive moietieslayer-by-layerspin-coatingfreestanding filmscatechol-modified polymersnacre-inspirednanocompositeScience & TechnologyFreestanding films based on catechol functionalized chitosan (CHI), hyaluronic acid (HA), and bioglass nanoparticles (BGNPs) were developed by spin-coating layer-by-layer assembly (SA-LbL). The catechol groups of 3,4-dihydroxy-<span style="font-variant: small-caps;">l</span>-phenylalanine (DOPA) present in the marine mussels adhesive proteins (MAPs) are the main factors responsible for their characteristic strong wet adhesion. Then, the produced films were cross-linked with genipin to improve their stability in wet state. Overall, the incorporation of BGNPs resulted in thicker and bioactive films, hydrophilic and rougher surfaces, reduced swelling, higher weight loss, and lower stiffness. The incorporation of catechol groups onto the films showed a significant increase in the films’ adhesion and stiffness, lower swelling, and weight loss. Interestingly, a synergetic effect on the stiffness increase was observed upon the combined incorporation of BGNPs with catechol-modified polymers, given that such films were the stiffest. Regarding the biological assays, the films exhibited no negative effects on cellular viability, adhesion, and proliferation, and the BGNPs seemed to promote higher cellular metabolic activity. These bioactive LbL freestanding films combine enhanced adhesion with improved mechanical properties and could find applications in the biomedical field, such as guided hard tissue regeneration membranes.This research was funded by the Portuguese Foundation for Science and Technology (FCT) and the European program FEDER/FEEI (PTDC/BTM-MAT/28123/2017 and PTDC/NAN-MAT/31036/2017) and FCT (MIT-EXPL/BIO/0089/2017).The authors acknowledge the Portuguese Foundation for Science and Technology (FCT) and the European program FEDER/FEEI for the financial support through projects PTDC/BTM-MAT/28123/2017 and PTDC/NAN-MAT/31036/2017. The authors also acknowledge FCT for the financial support through the exploratory project MIT-EXPL/BIO/0089/2017.Multidisciplinary Digital Publishing InstituteUniversidade do MinhoMoreira, JoanaVale, A. CatarinaPires, R. A.Botelho, GabrielaReis, R. L.Alves, N. M.2020-02-142020-02-14T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/64578engMoreira, J.; Vale, A.C.; A. Pires, R.; Botelho, G.; Reis, R.L.; Alves, N.M. Spin-Coated Polysaccharide-Based Multilayered Freestanding Films with Adhesive and Bioactive Moieties. Molecules 2020, 25, 840.1420-304910.3390/molecules2504084032075064https://www.mdpi.com/1420-3049/25/4/840info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:32:23Zoai:repositorium.sdum.uminho.pt:1822/64578Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:27:44.184849Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Spin-coated polysaccharide-based multilayered freestanding films with adhesive and bioactive moieties
title Spin-coated polysaccharide-based multilayered freestanding films with adhesive and bioactive moieties
spellingShingle Spin-coated polysaccharide-based multilayered freestanding films with adhesive and bioactive moieties
Moreira, Joana
layer-by-layer
spin-coating
freestanding films
catechol-modified polymers
nacre-inspired
nanocomposite
Science & Technology
title_short Spin-coated polysaccharide-based multilayered freestanding films with adhesive and bioactive moieties
title_full Spin-coated polysaccharide-based multilayered freestanding films with adhesive and bioactive moieties
title_fullStr Spin-coated polysaccharide-based multilayered freestanding films with adhesive and bioactive moieties
title_full_unstemmed Spin-coated polysaccharide-based multilayered freestanding films with adhesive and bioactive moieties
title_sort Spin-coated polysaccharide-based multilayered freestanding films with adhesive and bioactive moieties
author Moreira, Joana
author_facet Moreira, Joana
Vale, A. Catarina
Pires, R. A.
Botelho, Gabriela
Reis, R. L.
Alves, N. M.
author_role author
author2 Vale, A. Catarina
Pires, R. A.
Botelho, Gabriela
Reis, R. L.
Alves, N. M.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Moreira, Joana
Vale, A. Catarina
Pires, R. A.
Botelho, Gabriela
Reis, R. L.
Alves, N. M.
dc.subject.por.fl_str_mv layer-by-layer
spin-coating
freestanding films
catechol-modified polymers
nacre-inspired
nanocomposite
Science & Technology
topic layer-by-layer
spin-coating
freestanding films
catechol-modified polymers
nacre-inspired
nanocomposite
Science & Technology
description Freestanding films based on catechol functionalized chitosan (CHI), hyaluronic acid (HA), and bioglass nanoparticles (BGNPs) were developed by spin-coating layer-by-layer assembly (SA-LbL). The catechol groups of 3,4-dihydroxy-<span style="font-variant: small-caps;">l</span>-phenylalanine (DOPA) present in the marine mussels adhesive proteins (MAPs) are the main factors responsible for their characteristic strong wet adhesion. Then, the produced films were cross-linked with genipin to improve their stability in wet state. Overall, the incorporation of BGNPs resulted in thicker and bioactive films, hydrophilic and rougher surfaces, reduced swelling, higher weight loss, and lower stiffness. The incorporation of catechol groups onto the films showed a significant increase in the films’ adhesion and stiffness, lower swelling, and weight loss. Interestingly, a synergetic effect on the stiffness increase was observed upon the combined incorporation of BGNPs with catechol-modified polymers, given that such films were the stiffest. Regarding the biological assays, the films exhibited no negative effects on cellular viability, adhesion, and proliferation, and the BGNPs seemed to promote higher cellular metabolic activity. These bioactive LbL freestanding films combine enhanced adhesion with improved mechanical properties and could find applications in the biomedical field, such as guided hard tissue regeneration membranes.
publishDate 2020
dc.date.none.fl_str_mv 2020-02-14
2020-02-14T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/64578
url http://hdl.handle.net/1822/64578
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Moreira, J.; Vale, A.C.; A. Pires, R.; Botelho, G.; Reis, R.L.; Alves, N.M. Spin-Coated Polysaccharide-Based Multilayered Freestanding Films with Adhesive and Bioactive Moieties. Molecules 2020, 25, 840.
1420-3049
10.3390/molecules25040840
32075064
https://www.mdpi.com/1420-3049/25/4/840
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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