Aging is associated with impaired renal function, INF-gamma induced inflammation and with alterations in iron regulatory proteins gene expression

Detalhes bibliográficos
Autor(a) principal: Costa, E.
Data de Publicação: 2013
Outros Autores: Fernandes, J., Ribeiro, S., Garrido, P., Rocha-Pereira, P., Coimbra, S., Catarino, C., Reis, F., Belo, L., Bronze-da-Rocha, E., Vala, Helena, Alves, R., Santos-Silva, A.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.19/2727
Resumo: Our aim was to contribute to a better understanding of the pathophysiology of anemia in elderly, by studying how aging affects renal function, ironmetabolism, erythropoiesis and the inflammatory response, using an experimental animal model. The study was performed in male Wistar, a group of young rats with 2 months age and an old one with 18 months age. Old rats presented a significant higher urea, creatinine, interferon (INF)-gamma, ferritin and soluble transferrin receptor serum levels, as well as increased counts of reticulocytes and RDW. In addition, these rats showed significant lower erythropoietin (EPO) and iron serum levels. Concerning gene expression of iron regulatory proteins, old rats presented significantly higher mRNA levels of hepcidin (Hamp), transferrin (TF), transferrin receptor 2 (TfR2) and hemojuvelin (HJV); divalent metal transporter 1 (DMT1) mRNA levels were significantly higher in duodenal tissue; EPO gene expression was significantly higher in liver and lower in kidney, and the expression of the EPOR was significantly higher in both liver and kidney. Our results showed that aging is associated with impaired renal function, which could be in turn related with the inflammatory process and with a decline in EPO renal production. Moreover, we also propose that aging may be associatedwith INF-gamma-induced inflammation and with alterations upon iron regulatory proteins gene expression.
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spelling Aging is associated with impaired renal function, INF-gamma induced inflammation and with alterations in iron regulatory proteins gene expressionAnemiaelderlyerythropoietic disturbancesinflammationolder populationrenal failureOur aim was to contribute to a better understanding of the pathophysiology of anemia in elderly, by studying how aging affects renal function, ironmetabolism, erythropoiesis and the inflammatory response, using an experimental animal model. The study was performed in male Wistar, a group of young rats with 2 months age and an old one with 18 months age. Old rats presented a significant higher urea, creatinine, interferon (INF)-gamma, ferritin and soluble transferrin receptor serum levels, as well as increased counts of reticulocytes and RDW. In addition, these rats showed significant lower erythropoietin (EPO) and iron serum levels. Concerning gene expression of iron regulatory proteins, old rats presented significantly higher mRNA levels of hepcidin (Hamp), transferrin (TF), transferrin receptor 2 (TfR2) and hemojuvelin (HJV); divalent metal transporter 1 (DMT1) mRNA levels were significantly higher in duodenal tissue; EPO gene expression was significantly higher in liver and lower in kidney, and the expression of the EPOR was significantly higher in both liver and kidney. Our results showed that aging is associated with impaired renal function, which could be in turn related with the inflammatory process and with a decline in EPO renal production. Moreover, we also propose that aging may be associatedwith INF-gamma-induced inflammation and with alterations upon iron regulatory proteins gene expression.Repositório Científico do Instituto Politécnico de ViseuCosta, E.Fernandes, J.Ribeiro, S.Garrido, P.Rocha-Pereira, P.Coimbra, S.Catarino, C.Reis, F.Belo, L.Bronze-da-Rocha, E.Vala, HelenaAlves, R.Santos-Silva, A.2015-03-23T11:05:53Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.19/2727porCosta E, Fernandes J, Ribeiro S, Sereno J, Garrido P, Rocha-Pereira P, Coimbra S, Catarino C, Belo L, Bronze-da-Rocha E, Vala H, Alves R, Reis F, Santos-Silva A (2013). Aging is associated with impaired renal function, INF-gamma induced inflammation and with alterations in iron regulatory proteins gene expression Aging Dis: Dec 23;5(2):356-65 http://www.ncbi.nlm.nih.gov/pubmed/2548948810.14366/AD.2014.0500356info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-01-16T15:26:02ZPortal AgregadorONG
dc.title.none.fl_str_mv Aging is associated with impaired renal function, INF-gamma induced inflammation and with alterations in iron regulatory proteins gene expression
title Aging is associated with impaired renal function, INF-gamma induced inflammation and with alterations in iron regulatory proteins gene expression
spellingShingle Aging is associated with impaired renal function, INF-gamma induced inflammation and with alterations in iron regulatory proteins gene expression
Costa, E.
Anemia
elderly
erythropoietic disturbances
inflammation
older population
renal failure
title_short Aging is associated with impaired renal function, INF-gamma induced inflammation and with alterations in iron regulatory proteins gene expression
title_full Aging is associated with impaired renal function, INF-gamma induced inflammation and with alterations in iron regulatory proteins gene expression
title_fullStr Aging is associated with impaired renal function, INF-gamma induced inflammation and with alterations in iron regulatory proteins gene expression
title_full_unstemmed Aging is associated with impaired renal function, INF-gamma induced inflammation and with alterations in iron regulatory proteins gene expression
title_sort Aging is associated with impaired renal function, INF-gamma induced inflammation and with alterations in iron regulatory proteins gene expression
author Costa, E.
author_facet Costa, E.
Fernandes, J.
Ribeiro, S.
Garrido, P.
Rocha-Pereira, P.
Coimbra, S.
Catarino, C.
Reis, F.
Belo, L.
Bronze-da-Rocha, E.
Vala, Helena
Alves, R.
Santos-Silva, A.
author_role author
author2 Fernandes, J.
Ribeiro, S.
Garrido, P.
Rocha-Pereira, P.
Coimbra, S.
Catarino, C.
Reis, F.
Belo, L.
Bronze-da-Rocha, E.
Vala, Helena
Alves, R.
Santos-Silva, A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Politécnico de Viseu
dc.contributor.author.fl_str_mv Costa, E.
Fernandes, J.
Ribeiro, S.
Garrido, P.
Rocha-Pereira, P.
Coimbra, S.
Catarino, C.
Reis, F.
Belo, L.
Bronze-da-Rocha, E.
Vala, Helena
Alves, R.
Santos-Silva, A.
dc.subject.por.fl_str_mv Anemia
elderly
erythropoietic disturbances
inflammation
older population
renal failure
topic Anemia
elderly
erythropoietic disturbances
inflammation
older population
renal failure
description Our aim was to contribute to a better understanding of the pathophysiology of anemia in elderly, by studying how aging affects renal function, ironmetabolism, erythropoiesis and the inflammatory response, using an experimental animal model. The study was performed in male Wistar, a group of young rats with 2 months age and an old one with 18 months age. Old rats presented a significant higher urea, creatinine, interferon (INF)-gamma, ferritin and soluble transferrin receptor serum levels, as well as increased counts of reticulocytes and RDW. In addition, these rats showed significant lower erythropoietin (EPO) and iron serum levels. Concerning gene expression of iron regulatory proteins, old rats presented significantly higher mRNA levels of hepcidin (Hamp), transferrin (TF), transferrin receptor 2 (TfR2) and hemojuvelin (HJV); divalent metal transporter 1 (DMT1) mRNA levels were significantly higher in duodenal tissue; EPO gene expression was significantly higher in liver and lower in kidney, and the expression of the EPOR was significantly higher in both liver and kidney. Our results showed that aging is associated with impaired renal function, which could be in turn related with the inflammatory process and with a decline in EPO renal production. Moreover, we also propose that aging may be associatedwith INF-gamma-induced inflammation and with alterations upon iron regulatory proteins gene expression.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
2015-03-23T11:05:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.19/2727
url http://hdl.handle.net/10400.19/2727
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Costa E, Fernandes J, Ribeiro S, Sereno J, Garrido P, Rocha-Pereira P, Coimbra S, Catarino C, Belo L, Bronze-da-Rocha E, Vala H, Alves R, Reis F, Santos-Silva A (2013). Aging is associated with impaired renal function, INF-gamma induced inflammation and with alterations in iron regulatory proteins gene expression Aging Dis: Dec 23;5(2):356-65 http://www.ncbi.nlm.nih.gov/pubmed/25489488
10.14366/AD.2014.0500356
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.mail.fl_str_mv
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