With a little help from DNA barcoding

Bibliographic Details
Main Author: Borges, Luísa M. S.
Publication Date: 2016
Other Authors: Hollatz, Claudia, Lobo, Jorge, Cunha, Ana M., Vilela, Ana P., Calado, Gonçalo, Coelho, Rita, Costa, Ana C., Ferreira, Maria S. G., Costa, Maria H., Costa, Filipe O.
Format: Article
Language: eng
Source: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Download full: https://doi.org/10.1038/srep20226
Summary: The present study was financed by FEDER through POFC-COMPETE, in the scope the project FCOMP-01-0124-FEDER-015429 funded by "Fundacao para a Ciencia e a Tecnologia" (FCT), Portugal. Work at CBMA was supported by FCT I.P. through the strategic funding UID/BIA/04050/2013. Sequencing at the Biodiversity Institute of Ontario was funded by the International Barcode of Life (iBOL), through the Canadian Centre for DNA Barcoding, from the Ontario Genomics Institute, Genome Canada, the Ontario Ministry of Research and Innovation, and the Natural Sciences and Engineering Research Council of Canada. Claudia Hollatz was supported by a CAPES Post-doctoral fellowship (Ministry of Education, Brazil), while Jorge Lobo was supported by a PhD fellowship (SFRH/BD/69750/2010) from FCT.
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spelling With a little help from DNA barcodingInvestigating the diversity of Gastropoda from the Portuguese coastcytochrome c oxidaseGeneralSDG 14 - Life Below WaterThe present study was financed by FEDER through POFC-COMPETE, in the scope the project FCOMP-01-0124-FEDER-015429 funded by "Fundacao para a Ciencia e a Tecnologia" (FCT), Portugal. Work at CBMA was supported by FCT I.P. through the strategic funding UID/BIA/04050/2013. Sequencing at the Biodiversity Institute of Ontario was funded by the International Barcode of Life (iBOL), through the Canadian Centre for DNA Barcoding, from the Ontario Genomics Institute, Genome Canada, the Ontario Ministry of Research and Innovation, and the Natural Sciences and Engineering Research Council of Canada. Claudia Hollatz was supported by a CAPES Post-doctoral fellowship (Ministry of Education, Brazil), while Jorge Lobo was supported by a PhD fellowship (SFRH/BD/69750/2010) from FCT.The Gastropoda is one of the best studied classes of marine invertebrates. Yet, most species have been delimited based on morphology only. The application of DNA barcodes has shown to be greatly useful to help delimiting species. Therefore, sequences of the cytochrome c oxidase I gene from 108 specimens of 34 morpho-species were used to investigate the molecular diversity within the gastropods from the Portuguese coast. To the above dataset, we added available COI-5P sequences of taxonomically close species, in a total of 58 morpho-species examined. There was a good match between ours and sequences from independent studies, in public repositories. We found 32 concordant (91.4%) out of the 35 Barcode Index Numbers (BINs) generated from our sequences. The application of a ranking system to the barcodes yield over 70% with top taxonomic congruence, while 14.2% of the species barcodes had insufficient data. In the majority of the cases, there was a good concordance between morphological identification and DNA barcodes. Nonetheless, the discordance between morphological and molecular data is a reminder that even the comparatively well-known European marine gastropods can benefit from being probed using the DNA barcode approach. Discordant cases should be reviewed with more integrative studies.DCEA - Departamento de Ciências e Engenharia do AmbienteRUNBorges, Luísa M. S.Hollatz, ClaudiaLobo, JorgeCunha, Ana M.Vilela, Ana P.Calado, GonçaloCoelho, RitaCosta, Ana C.Ferreira, Maria S. G.Costa, Maria H.Costa, Filipe O.2018-05-09T22:04:44Z2016-02-152016-02-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1038/srep20226engPURE: 1637622http://www.scopus.com/inward/record.url?scp=84958280148&partnerID=8YFLogxKhttps://doi.org/10.1038/srep20226info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:19:41Zoai:run.unl.pt:10362/36311Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:26.288545Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv With a little help from DNA barcoding
Investigating the diversity of Gastropoda from the Portuguese coast
title With a little help from DNA barcoding
spellingShingle With a little help from DNA barcoding
Borges, Luísa M. S.
cytochrome c oxidase
General
SDG 14 - Life Below Water
title_short With a little help from DNA barcoding
title_full With a little help from DNA barcoding
title_fullStr With a little help from DNA barcoding
title_full_unstemmed With a little help from DNA barcoding
title_sort With a little help from DNA barcoding
author Borges, Luísa M. S.
author_facet Borges, Luísa M. S.
Hollatz, Claudia
Lobo, Jorge
Cunha, Ana M.
Vilela, Ana P.
Calado, Gonçalo
Coelho, Rita
Costa, Ana C.
Ferreira, Maria S. G.
Costa, Maria H.
Costa, Filipe O.
author_role author
author2 Hollatz, Claudia
Lobo, Jorge
Cunha, Ana M.
Vilela, Ana P.
Calado, Gonçalo
Coelho, Rita
Costa, Ana C.
Ferreira, Maria S. G.
Costa, Maria H.
Costa, Filipe O.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv DCEA - Departamento de Ciências e Engenharia do Ambiente
RUN
dc.contributor.author.fl_str_mv Borges, Luísa M. S.
Hollatz, Claudia
Lobo, Jorge
Cunha, Ana M.
Vilela, Ana P.
Calado, Gonçalo
Coelho, Rita
Costa, Ana C.
Ferreira, Maria S. G.
Costa, Maria H.
Costa, Filipe O.
dc.subject.por.fl_str_mv cytochrome c oxidase
General
SDG 14 - Life Below Water
topic cytochrome c oxidase
General
SDG 14 - Life Below Water
description The present study was financed by FEDER through POFC-COMPETE, in the scope the project FCOMP-01-0124-FEDER-015429 funded by "Fundacao para a Ciencia e a Tecnologia" (FCT), Portugal. Work at CBMA was supported by FCT I.P. through the strategic funding UID/BIA/04050/2013. Sequencing at the Biodiversity Institute of Ontario was funded by the International Barcode of Life (iBOL), through the Canadian Centre for DNA Barcoding, from the Ontario Genomics Institute, Genome Canada, the Ontario Ministry of Research and Innovation, and the Natural Sciences and Engineering Research Council of Canada. Claudia Hollatz was supported by a CAPES Post-doctoral fellowship (Ministry of Education, Brazil), while Jorge Lobo was supported by a PhD fellowship (SFRH/BD/69750/2010) from FCT.
publishDate 2016
dc.date.none.fl_str_mv 2016-02-15
2016-02-15T00:00:00Z
2018-05-09T22:04:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1038/srep20226
url https://doi.org/10.1038/srep20226
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PURE: 1637622
http://www.scopus.com/inward/record.url?scp=84958280148&partnerID=8YFLogxK
https://doi.org/10.1038/srep20226
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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