Differential proteomic analysis of lactic acid bacteria - Escherichia coli O157:H7 interaction and its contribution to bioprotection strategies in meat

Detalhes bibliográficos
Autor(a) principal: Orihuel, Alejandra
Data de Publicação: 2018
Outros Autores: Terán, Lucrecia, Renaut, Jenny, Vignolo, Graciela M., Almeida, André M. de, Saavedra, Maria L., Fadda, Silvina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/15761
Resumo: Original Research
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spelling Differential proteomic analysis of lactic acid bacteria - Escherichia coli O157:H7 interaction and its contribution to bioprotection strategies in meatlactic acid bacteria (LAB)bioprotective culturesenterohemorrhagic Escherichia coli (EHECmeat safetybacterial interactionproteomicsOriginal ResearchHuman infection by Enterohemorrhagic Escherichia (E.) coli (EHEC) occurs through the ingestion of contaminated foods such as milk, vegetable products, water-based drinks, and particularly minced meats. Indeed EHEC is a pathogen that threatens public health and meat industry. The potential of different Lactic Acid Bacteria (LAB) strains to control EHEC in a meat-based medium was evaluated by using a simple and rapid method and by analyzing the growth kinetics of co-cultures (LAB-EHEC) in a meat-based medium. The activity of LAB toward EHEC in co-cultures showed variable inhibitory effect. Although, LAB were able to control EHEC, neither the produced acid nor bacteriocins were responsible of the inhibition. The bacteriocinogenic Enteroccus (Ent.) mundtii CRL35 presented one of the highest inhibition activities. A proteomic approach was used to evaluate bacterial interaction and antagonistic mechanisms between Ent. mundtii and EHEC. Physiological observations, such as growth kinetics, acidification ability and EHEC inhibitory potential were supported by the proteomic results, demonstrating significant differences in protein expression in LAB: (i) due to the presence of the pathogen and (ii) according to the growth phase analyzed. Most of the identified proteins belonged to carbohydrate/amino acid metabolism, energy production, transcription/translation, and cell division. These results contribute to the knowledge of competition strategies used by Ent. mundtii during its co-culture with EHEC setting new perspectives for the use of LAB to control this pathogen in meatAldo CorsettiRepositório da Universidade de LisboaOrihuel, AlejandraTerán, LucreciaRenaut, JennyVignolo, Graciela M.Almeida, André M. deSaavedra, Maria L.Fadda, Silvina2018-07-02T12:47:56Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/15761engOrihuel A, Terán L, Renaut J, Vignolo GM, De Almeida AM, Saavedra ML and Fadda S (2018) Differential Proteomic Analysis of Lactic Acid Bacteria—Escherichia coli O157:H7 Interaction and Its Contribution to Bioprotection Strategies in Meat. Front. Microbiol. 9:108310.3389/fmicb.2018.01083info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:45:40ZPortal AgregadorONG
dc.title.none.fl_str_mv Differential proteomic analysis of lactic acid bacteria - Escherichia coli O157:H7 interaction and its contribution to bioprotection strategies in meat
title Differential proteomic analysis of lactic acid bacteria - Escherichia coli O157:H7 interaction and its contribution to bioprotection strategies in meat
spellingShingle Differential proteomic analysis of lactic acid bacteria - Escherichia coli O157:H7 interaction and its contribution to bioprotection strategies in meat
Orihuel, Alejandra
lactic acid bacteria (LAB)
bioprotective cultures
enterohemorrhagic Escherichia coli (EHEC
meat safety
bacterial interaction
proteomics
title_short Differential proteomic analysis of lactic acid bacteria - Escherichia coli O157:H7 interaction and its contribution to bioprotection strategies in meat
title_full Differential proteomic analysis of lactic acid bacteria - Escherichia coli O157:H7 interaction and its contribution to bioprotection strategies in meat
title_fullStr Differential proteomic analysis of lactic acid bacteria - Escherichia coli O157:H7 interaction and its contribution to bioprotection strategies in meat
title_full_unstemmed Differential proteomic analysis of lactic acid bacteria - Escherichia coli O157:H7 interaction and its contribution to bioprotection strategies in meat
title_sort Differential proteomic analysis of lactic acid bacteria - Escherichia coli O157:H7 interaction and its contribution to bioprotection strategies in meat
author Orihuel, Alejandra
author_facet Orihuel, Alejandra
Terán, Lucrecia
Renaut, Jenny
Vignolo, Graciela M.
Almeida, André M. de
Saavedra, Maria L.
Fadda, Silvina
author_role author
author2 Terán, Lucrecia
Renaut, Jenny
Vignolo, Graciela M.
Almeida, André M. de
Saavedra, Maria L.
Fadda, Silvina
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Orihuel, Alejandra
Terán, Lucrecia
Renaut, Jenny
Vignolo, Graciela M.
Almeida, André M. de
Saavedra, Maria L.
Fadda, Silvina
dc.subject.por.fl_str_mv lactic acid bacteria (LAB)
bioprotective cultures
enterohemorrhagic Escherichia coli (EHEC
meat safety
bacterial interaction
proteomics
topic lactic acid bacteria (LAB)
bioprotective cultures
enterohemorrhagic Escherichia coli (EHEC
meat safety
bacterial interaction
proteomics
description Original Research
publishDate 2018
dc.date.none.fl_str_mv 2018-07-02T12:47:56Z
2018
2018-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/15761
url http://hdl.handle.net/10400.5/15761
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Orihuel A, Terán L, Renaut J, Vignolo GM, De Almeida AM, Saavedra ML and Fadda S (2018) Differential Proteomic Analysis of Lactic Acid Bacteria—Escherichia coli O157:H7 Interaction and Its Contribution to Bioprotection Strategies in Meat. Front. Microbiol. 9:1083
10.3389/fmicb.2018.01083
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Aldo Corsetti
publisher.none.fl_str_mv Aldo Corsetti
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1777302145649868800

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