Doping β-TCP as a Strategy for Enhancing the Regenerative Potential of Composite β-TCP-Alkali-Free Bioactive Glass Bone Grafts. Experimental Study in Rats

Detalhes bibliográficos
Autor(a) principal: Ferreira, Manuel M.
Data de Publicação: 2018
Outros Autores: Brito, Ana F., Brazete, Daniela, Pereira, Inês C., Carrilho, Eunice, Abrantes, Ana M., Pires, Ana S., Aguiar, Maria J, Carvalho, Lina, Botelho, Filomena, Ferreira, José M. F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/107767
https://doi.org/10.3390/ma12010004
Resumo: The present work aims at evaluating the potential gains derived from partially replacing calcium in resorbable β-tricalcium phosphate (β-TCP) by two different molar percentages of strontium (5, 10) and zinc (1, 2), concomitantly with a fixed molar percentage (0.5) of manganese. Synthetic granular composite bone filling grafts consisting of doped β-TCP and an alkali-free bioactive glass were prepared and implanted in ~4 mm diameter bone defects drilled in the calvaria of Wistar rats used as animal models. The animals were sacrificed after 9 weeks of implantation and the calvaria was excised. Non-manipulated bone was used as positive control, while empty defects were used as a negative control group. The von Kossa staining revealed an enhanced new bone formation with increasing doping levels, supporting the therapeutic effects exerted by the doping elements. The percentage of newly formed bone was similar when the defects were filled with autologous bone, BG (previous results) or 3TCP2/7BG, which indicates that the latter two are excellent candidates for replacement of autologous bone as bone regeneration material. This finding confirms that doping with suitable doses of therapeutic ions is a good strategy towards transposing the bone graft materials to biomedical applications in humans.
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spelling Doping β-TCP as a Strategy for Enhancing the Regenerative Potential of Composite β-TCP-Alkali-Free Bioactive Glass Bone Grafts. Experimental Study in Ratscomposite bone graftscalcium phosphatesimplantsdentistrybiomedical engineeringosteogenesisThe present work aims at evaluating the potential gains derived from partially replacing calcium in resorbable β-tricalcium phosphate (β-TCP) by two different molar percentages of strontium (5, 10) and zinc (1, 2), concomitantly with a fixed molar percentage (0.5) of manganese. Synthetic granular composite bone filling grafts consisting of doped β-TCP and an alkali-free bioactive glass were prepared and implanted in ~4 mm diameter bone defects drilled in the calvaria of Wistar rats used as animal models. The animals were sacrificed after 9 weeks of implantation and the calvaria was excised. Non-manipulated bone was used as positive control, while empty defects were used as a negative control group. The von Kossa staining revealed an enhanced new bone formation with increasing doping levels, supporting the therapeutic effects exerted by the doping elements. The percentage of newly formed bone was similar when the defects were filled with autologous bone, BG (previous results) or 3TCP2/7BG, which indicates that the latter two are excellent candidates for replacement of autologous bone as bone regeneration material. This finding confirms that doping with suitable doses of therapeutic ions is a good strategy towards transposing the bone graft materials to biomedical applications in humans.MDPI2018-12-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/107767http://hdl.handle.net/10316/107767https://doi.org/10.3390/ma12010004eng1996-194430577440Ferreira, Manuel M.Brito, Ana F.Brazete, DanielaPereira, Inês C.Carrilho, EuniceAbrantes, Ana M.Pires, Ana S.Aguiar, Maria JCarvalho, LinaBotelho, FilomenaFerreira, José M. F.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-01T09:12:46Zoai:estudogeral.uc.pt:10316/107767Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:24:04.641575Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Doping β-TCP as a Strategy for Enhancing the Regenerative Potential of Composite β-TCP-Alkali-Free Bioactive Glass Bone Grafts. Experimental Study in Rats
title Doping β-TCP as a Strategy for Enhancing the Regenerative Potential of Composite β-TCP-Alkali-Free Bioactive Glass Bone Grafts. Experimental Study in Rats
spellingShingle Doping β-TCP as a Strategy for Enhancing the Regenerative Potential of Composite β-TCP-Alkali-Free Bioactive Glass Bone Grafts. Experimental Study in Rats
Ferreira, Manuel M.
composite bone grafts
calcium phosphates
implants
dentistry
biomedical engineering
osteogenesis
title_short Doping β-TCP as a Strategy for Enhancing the Regenerative Potential of Composite β-TCP-Alkali-Free Bioactive Glass Bone Grafts. Experimental Study in Rats
title_full Doping β-TCP as a Strategy for Enhancing the Regenerative Potential of Composite β-TCP-Alkali-Free Bioactive Glass Bone Grafts. Experimental Study in Rats
title_fullStr Doping β-TCP as a Strategy for Enhancing the Regenerative Potential of Composite β-TCP-Alkali-Free Bioactive Glass Bone Grafts. Experimental Study in Rats
title_full_unstemmed Doping β-TCP as a Strategy for Enhancing the Regenerative Potential of Composite β-TCP-Alkali-Free Bioactive Glass Bone Grafts. Experimental Study in Rats
title_sort Doping β-TCP as a Strategy for Enhancing the Regenerative Potential of Composite β-TCP-Alkali-Free Bioactive Glass Bone Grafts. Experimental Study in Rats
author Ferreira, Manuel M.
author_facet Ferreira, Manuel M.
Brito, Ana F.
Brazete, Daniela
Pereira, Inês C.
Carrilho, Eunice
Abrantes, Ana M.
Pires, Ana S.
Aguiar, Maria J
Carvalho, Lina
Botelho, Filomena
Ferreira, José M. F.
author_role author
author2 Brito, Ana F.
Brazete, Daniela
Pereira, Inês C.
Carrilho, Eunice
Abrantes, Ana M.
Pires, Ana S.
Aguiar, Maria J
Carvalho, Lina
Botelho, Filomena
Ferreira, José M. F.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira, Manuel M.
Brito, Ana F.
Brazete, Daniela
Pereira, Inês C.
Carrilho, Eunice
Abrantes, Ana M.
Pires, Ana S.
Aguiar, Maria J
Carvalho, Lina
Botelho, Filomena
Ferreira, José M. F.
dc.subject.por.fl_str_mv composite bone grafts
calcium phosphates
implants
dentistry
biomedical engineering
osteogenesis
topic composite bone grafts
calcium phosphates
implants
dentistry
biomedical engineering
osteogenesis
description The present work aims at evaluating the potential gains derived from partially replacing calcium in resorbable β-tricalcium phosphate (β-TCP) by two different molar percentages of strontium (5, 10) and zinc (1, 2), concomitantly with a fixed molar percentage (0.5) of manganese. Synthetic granular composite bone filling grafts consisting of doped β-TCP and an alkali-free bioactive glass were prepared and implanted in ~4 mm diameter bone defects drilled in the calvaria of Wistar rats used as animal models. The animals were sacrificed after 9 weeks of implantation and the calvaria was excised. Non-manipulated bone was used as positive control, while empty defects were used as a negative control group. The von Kossa staining revealed an enhanced new bone formation with increasing doping levels, supporting the therapeutic effects exerted by the doping elements. The percentage of newly formed bone was similar when the defects were filled with autologous bone, BG (previous results) or 3TCP2/7BG, which indicates that the latter two are excellent candidates for replacement of autologous bone as bone regeneration material. This finding confirms that doping with suitable doses of therapeutic ions is a good strategy towards transposing the bone graft materials to biomedical applications in humans.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-20
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/107767
http://hdl.handle.net/10316/107767
https://doi.org/10.3390/ma12010004
url http://hdl.handle.net/10316/107767
https://doi.org/10.3390/ma12010004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1996-1944
30577440
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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