Differential sensitivities to lactate transport inhibitors of breast cancer cell lines

Detalhes bibliográficos
Autor(a) principal: Santos, Filipa Morais
Data de Publicação: 2014
Outros Autores: Gonçalves, Vera M., Pinheiro, Sílvia, Vieira, André Filipe, Paredes, Joana, Schmitt, Fernando C., Baltazar, Fátima, Pinheiro, Céline
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/32898
Resumo: This is linked to the online version of the paper at: http://dx.doi.org/10.1530/ERC-13-0132.
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spelling Differential sensitivities to lactate transport inhibitors of breast cancer cell linesBreastMolecular biologyCarcinomaScience & TechnologyThis is linked to the online version of the paper at: http://dx.doi.org/10.1530/ERC-13-0132.The tumour microenvironment is known to be acidic due to high glycolytic rates of tumour cells. Monocarboxylate transporters (MCTs) play a role in extracellular acidification, which is widely known to be involved in tumour progression. Recently, we have described the upregulation of MCT1 in breast carcinomas and its association with poor prognostic variables. Thus, we aimed to evaluate the effect of lactate transport inhibition in human breast cancer cell lines. The effects of a-cyano-4-hydroxycinnamate, quercetin and lonidamine on cell viability, metabolism, proliferation, apoptosis, migration and invasion were assessed in a panel of different breast cancer cell lines. MCT1, MCT4 and CD147 were differently expressed among the breast cancer cell lines and, as expected, different sensitivities were observed for the three inhibitors. Interestingly, in the most sensitive cell lines, lactate transport inhibition induced a decrease in cell proliferation, migration and invasion, as well as an increase in cell death. Results were validated by silencing MCT1 expression using siRNA. The results obtained here support targeting of lactate transport as a strategy to treat breast cancer, with a special emphasis on the basal-like subtype, which so far does not have a specific molecular therapy.This work was supported by the Fundação para a Ciência e a Tecnologia (FCT) grant ref. PTDC/SAU-FCF/104347/2008, under the scope of ‘Programa Operacional Temático Factores de Competitividade’ (COMPETE) of ‘Quadro Comunitário de Apoio III’ and co-financed by the Fundo Europeu De Desenvolvimento Regional (FEDER).Society for EndocrinologyUniversidade do MinhoSantos, Filipa MoraisGonçalves, Vera M.Pinheiro, SílviaVieira, André FilipeParedes, JoanaSchmitt, Fernando C.Baltazar, FátimaPinheiro, Céline20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/32898eng1351-008810.1530/ERC-13-013224174370http://erc.endocrinology-journals.org/cgi/pmidlookup?view=long&pmid=24174370info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:10:20ZPortal AgregadorONG
dc.title.none.fl_str_mv Differential sensitivities to lactate transport inhibitors of breast cancer cell lines
title Differential sensitivities to lactate transport inhibitors of breast cancer cell lines
spellingShingle Differential sensitivities to lactate transport inhibitors of breast cancer cell lines
Santos, Filipa Morais
Breast
Molecular biology
Carcinoma
Science & Technology
title_short Differential sensitivities to lactate transport inhibitors of breast cancer cell lines
title_full Differential sensitivities to lactate transport inhibitors of breast cancer cell lines
title_fullStr Differential sensitivities to lactate transport inhibitors of breast cancer cell lines
title_full_unstemmed Differential sensitivities to lactate transport inhibitors of breast cancer cell lines
title_sort Differential sensitivities to lactate transport inhibitors of breast cancer cell lines
author Santos, Filipa Morais
author_facet Santos, Filipa Morais
Gonçalves, Vera M.
Pinheiro, Sílvia
Vieira, André Filipe
Paredes, Joana
Schmitt, Fernando C.
Baltazar, Fátima
Pinheiro, Céline
author_role author
author2 Gonçalves, Vera M.
Pinheiro, Sílvia
Vieira, André Filipe
Paredes, Joana
Schmitt, Fernando C.
Baltazar, Fátima
Pinheiro, Céline
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Santos, Filipa Morais
Gonçalves, Vera M.
Pinheiro, Sílvia
Vieira, André Filipe
Paredes, Joana
Schmitt, Fernando C.
Baltazar, Fátima
Pinheiro, Céline
dc.subject.por.fl_str_mv Breast
Molecular biology
Carcinoma
Science & Technology
topic Breast
Molecular biology
Carcinoma
Science & Technology
description This is linked to the online version of the paper at: http://dx.doi.org/10.1530/ERC-13-0132.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/32898
url http://hdl.handle.net/1822/32898
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1351-0088
10.1530/ERC-13-0132
24174370
http://erc.endocrinology-journals.org/cgi/pmidlookup?view=long&pmid=24174370
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Society for Endocrinology
publisher.none.fl_str_mv Society for Endocrinology
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instacron_str RCAAP
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.mail.fl_str_mv
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