Age-dependent energy metabolism and transcriptome changes in urine-derived stem cells

Detalhes bibliográficos
Autor(a) principal: Ferreiro, Elisabete
Data de Publicação: 2024
Outros Autores: Monteiro, Mariana, Pereira, Francisco, Barroso, Cristina, Egas, Conceição, Macedo, Paula, Valero, Jorge, Sardão, Vilma A., Oliveira, Paulo J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/163605
Resumo: Funding Information: This work was financed by the European Regional Development Fund ( ERDF ), through the Centro 2020 Regional Operational Programme, through the COMPETE 2020 - Operational Programme for Competitiveness and Internationalisation and Portuguese national funds via FCT – Fundação para a Ciência e a Tecnologia, under the project PTDC/BTM-SAL/29297/2017 , PTDC/MED-FAR/29391/2017 , PTDC/BTM-ORG/0055/2021 ( https://doi.org/10.54499/PTDC/BTM-ORG/0055/2021 ), POCI-01–0145-FEDER-029297 , POCI-01–0145-FEDER-029391 , POCI-01–0145-FEDER-022184 and UIDB/04539/2020 , UIDP/04539/2020 and LA/P/0058/2020 . EF was financed by Assistant Researcher contract from FCT ( CEECIND/00322/2017 https://doi.org/10.54499/CEECIND/00322/2017/CP1390/CT0001 ) and is supported by Assistant Researcher contracts from FCT ( 2022.00011 . CEECIND ). VS was supported by an Assistant Researcher contract from FCT (IF/01182/2015). The authors would like to thank all donors involved in the study. Publisher Copyright: © 2024 The Authors
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spelling Age-dependent energy metabolism and transcriptome changes in urine-derived stem cellsAgingMachine learningMetabolismMitochondriaTranscriptomeUrine-derived stem cellsWomenAgeingDevelopmental BiologyFunding Information: This work was financed by the European Regional Development Fund ( ERDF ), through the Centro 2020 Regional Operational Programme, through the COMPETE 2020 - Operational Programme for Competitiveness and Internationalisation and Portuguese national funds via FCT – Fundação para a Ciência e a Tecnologia, under the project PTDC/BTM-SAL/29297/2017 , PTDC/MED-FAR/29391/2017 , PTDC/BTM-ORG/0055/2021 ( https://doi.org/10.54499/PTDC/BTM-ORG/0055/2021 ), POCI-01–0145-FEDER-029297 , POCI-01–0145-FEDER-029391 , POCI-01–0145-FEDER-022184 and UIDB/04539/2020 , UIDP/04539/2020 and LA/P/0058/2020 . EF was financed by Assistant Researcher contract from FCT ( CEECIND/00322/2017 https://doi.org/10.54499/CEECIND/00322/2017/CP1390/CT0001 ) and is supported by Assistant Researcher contracts from FCT ( 2022.00011 . CEECIND ). VS was supported by an Assistant Researcher contract from FCT (IF/01182/2015). The authors would like to thank all donors involved in the study. Publisher Copyright: © 2024 The AuthorsThe global population over 60 years old is projected to reach 1.5 billion by 2050. Understanding age-related disorders and gender-specificities is crucial for a healthy aging. Reliable age-related biomarkers are needed, preferentially obtained through non-invasive methods. Urine-derived stem cells (UDSCs) can be easily obtained, although a detailed bioenergetic characterization, according to the donor aging, remain unexplored. UDSCs were isolated from young and elderly adult women (22–35 and 70–94 years old, respectively). Surprisingly, UDSCs from elderly subjects exhibited significantly higher maximal oxygen consumption and bioenergetic health index than those from younger individuals, evaluated through oxygen consumption rate. Exploratory data analysis methods were applied to engineer a minimal subset of features for the classification and stratification of UDSCs. Additionally, RNAseq of UDSCs was performed to identify age-related transcriptional changes. Transcriptional analysis revealed downregulation of genes related to glucuronidation and estrogen metabolism, and upregulation of inflammation-related genes in UDSCs from elderly individuals. This study demonstrates unexpected differences in the UDSCs’ OCR between young and elderly individuals, revealing improved bioenergetics in concurrent with an aged-like transcriptome signature. UDSCs offer a non-invasive model for studying age-related changes, holding promise for aging research and therapeutic studies.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)RUNFerreiro, ElisabeteMonteiro, MarianaPereira, FranciscoBarroso, CristinaEgas, ConceiçãoMacedo, PaulaValero, JorgeSardão, Vilma A.Oliveira, Paulo J.2024-02-16T01:10:41Z2024-042024-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/163605eng0047-6374PURE: 82800032https://doi.org/10.1016/j.mad.2024.111912info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:47:44Zoai:run.unl.pt:10362/163605Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:59:29.199515Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Age-dependent energy metabolism and transcriptome changes in urine-derived stem cells
title Age-dependent energy metabolism and transcriptome changes in urine-derived stem cells
spellingShingle Age-dependent energy metabolism and transcriptome changes in urine-derived stem cells
Ferreiro, Elisabete
Aging
Machine learning
Metabolism
Mitochondria
Transcriptome
Urine-derived stem cells
Women
Ageing
Developmental Biology
title_short Age-dependent energy metabolism and transcriptome changes in urine-derived stem cells
title_full Age-dependent energy metabolism and transcriptome changes in urine-derived stem cells
title_fullStr Age-dependent energy metabolism and transcriptome changes in urine-derived stem cells
title_full_unstemmed Age-dependent energy metabolism and transcriptome changes in urine-derived stem cells
title_sort Age-dependent energy metabolism and transcriptome changes in urine-derived stem cells
author Ferreiro, Elisabete
author_facet Ferreiro, Elisabete
Monteiro, Mariana
Pereira, Francisco
Barroso, Cristina
Egas, Conceição
Macedo, Paula
Valero, Jorge
Sardão, Vilma A.
Oliveira, Paulo J.
author_role author
author2 Monteiro, Mariana
Pereira, Francisco
Barroso, Cristina
Egas, Conceição
Macedo, Paula
Valero, Jorge
Sardão, Vilma A.
Oliveira, Paulo J.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
RUN
dc.contributor.author.fl_str_mv Ferreiro, Elisabete
Monteiro, Mariana
Pereira, Francisco
Barroso, Cristina
Egas, Conceição
Macedo, Paula
Valero, Jorge
Sardão, Vilma A.
Oliveira, Paulo J.
dc.subject.por.fl_str_mv Aging
Machine learning
Metabolism
Mitochondria
Transcriptome
Urine-derived stem cells
Women
Ageing
Developmental Biology
topic Aging
Machine learning
Metabolism
Mitochondria
Transcriptome
Urine-derived stem cells
Women
Ageing
Developmental Biology
description Funding Information: This work was financed by the European Regional Development Fund ( ERDF ), through the Centro 2020 Regional Operational Programme, through the COMPETE 2020 - Operational Programme for Competitiveness and Internationalisation and Portuguese national funds via FCT – Fundação para a Ciência e a Tecnologia, under the project PTDC/BTM-SAL/29297/2017 , PTDC/MED-FAR/29391/2017 , PTDC/BTM-ORG/0055/2021 ( https://doi.org/10.54499/PTDC/BTM-ORG/0055/2021 ), POCI-01–0145-FEDER-029297 , POCI-01–0145-FEDER-029391 , POCI-01–0145-FEDER-022184 and UIDB/04539/2020 , UIDP/04539/2020 and LA/P/0058/2020 . EF was financed by Assistant Researcher contract from FCT ( CEECIND/00322/2017 https://doi.org/10.54499/CEECIND/00322/2017/CP1390/CT0001 ) and is supported by Assistant Researcher contracts from FCT ( 2022.00011 . CEECIND ). VS was supported by an Assistant Researcher contract from FCT (IF/01182/2015). The authors would like to thank all donors involved in the study. Publisher Copyright: © 2024 The Authors
publishDate 2024
dc.date.none.fl_str_mv 2024-02-16T01:10:41Z
2024-04
2024-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/163605
url http://hdl.handle.net/10362/163605
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0047-6374
PURE: 82800032
https://doi.org/10.1016/j.mad.2024.111912
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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