Natural immobilisation of microorganisms for continuous ethanol production

Detalhes bibliográficos
Autor(a) principal: Baptista, C. M. S. G.
Data de Publicação: 2006
Outros Autores: Cóias, J. M. A., Oliveira, A. C. M., Oliveira, N. M. C., Rocha, J. M. S., Dempsey, M. J., Lannigan, K. C., Benson, P. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/3792
https://doi.org/10.1016/j.enzmictec.2005.12.025
Resumo: Using a growth medium based on cane blackstrap molasses, we compared ethanol production by two strains of Saccharomyces cerevisiae that were immobilized in polyurethane foam cubes in a fluidised-bed fermenter. One strain (NCYC 1119) was adhesive and extremely flocculent, whilst the other strain was not adhesive and only weakly flocculent. The strong flocs of NCYC 1119 caused blockage of the bed, so that stable operation could not be achieved beyond 15 days. Nevertheless, it was able to produce 40 g L-1 ethanol at a rate up to 16 g L-1 h-1 (D = 0.4 h-1), although this production period was limited to 192 h. In contrast, the non-adhesive strain was only capable of producing 28 g L-1 ethanol at a rate of 11 g L-1 h-1 at the same dilution rate, even though production continued for 576 h. Despite the conversion of sugars to ethanol not being complete during these trials (up to 47 g L-1 was expected), it was clearly demonstrated that the productivity of the adhesive strain was higher than that of the non-adhesive one. However, further work is required to develop this process into a robust, industrial system.
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spelling Natural immobilisation of microorganisms for continuous ethanol productionBioethanolFluidized bedMolassesTransport fuel2003/30/ECImmobilized cellUsing a growth medium based on cane blackstrap molasses, we compared ethanol production by two strains of Saccharomyces cerevisiae that were immobilized in polyurethane foam cubes in a fluidised-bed fermenter. One strain (NCYC 1119) was adhesive and extremely flocculent, whilst the other strain was not adhesive and only weakly flocculent. The strong flocs of NCYC 1119 caused blockage of the bed, so that stable operation could not be achieved beyond 15 days. Nevertheless, it was able to produce 40 g L-1 ethanol at a rate up to 16 g L-1 h-1 (D = 0.4 h-1), although this production period was limited to 192 h. In contrast, the non-adhesive strain was only capable of producing 28 g L-1 ethanol at a rate of 11 g L-1 h-1 at the same dilution rate, even though production continued for 576 h. Despite the conversion of sugars to ethanol not being complete during these trials (up to 47 g L-1 was expected), it was clearly demonstrated that the productivity of the adhesive strain was higher than that of the non-adhesive one. However, further work is required to develop this process into a robust, industrial system.http://www.sciencedirect.com/science/article/B6TG1-4K3CKGW-6/1/e0acf7d19e1fcfcc4e0bfeabfc9f39e52006info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleaplication/PDFhttp://hdl.handle.net/10316/3792http://hdl.handle.net/10316/3792https://doi.org/10.1016/j.enzmictec.2005.12.025engEnzyme and Microbial Technology. 40:1 (2006) 127-131Baptista, C. M. S. G.Cóias, J. M. A.Oliveira, A. C. M.Oliveira, N. M. C.Rocha, J. M. S.Dempsey, M. J.Lannigan, K. C.Benson, P. S.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-11-06T16:49:03Zoai:estudogeral.uc.pt:10316/3792Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:59:13.383466Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Natural immobilisation of microorganisms for continuous ethanol production
title Natural immobilisation of microorganisms for continuous ethanol production
spellingShingle Natural immobilisation of microorganisms for continuous ethanol production
Baptista, C. M. S. G.
Bioethanol
Fluidized bed
Molasses
Transport fuel
2003/30/EC
Immobilized cell
title_short Natural immobilisation of microorganisms for continuous ethanol production
title_full Natural immobilisation of microorganisms for continuous ethanol production
title_fullStr Natural immobilisation of microorganisms for continuous ethanol production
title_full_unstemmed Natural immobilisation of microorganisms for continuous ethanol production
title_sort Natural immobilisation of microorganisms for continuous ethanol production
author Baptista, C. M. S. G.
author_facet Baptista, C. M. S. G.
Cóias, J. M. A.
Oliveira, A. C. M.
Oliveira, N. M. C.
Rocha, J. M. S.
Dempsey, M. J.
Lannigan, K. C.
Benson, P. S.
author_role author
author2 Cóias, J. M. A.
Oliveira, A. C. M.
Oliveira, N. M. C.
Rocha, J. M. S.
Dempsey, M. J.
Lannigan, K. C.
Benson, P. S.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Baptista, C. M. S. G.
Cóias, J. M. A.
Oliveira, A. C. M.
Oliveira, N. M. C.
Rocha, J. M. S.
Dempsey, M. J.
Lannigan, K. C.
Benson, P. S.
dc.subject.por.fl_str_mv Bioethanol
Fluidized bed
Molasses
Transport fuel
2003/30/EC
Immobilized cell
topic Bioethanol
Fluidized bed
Molasses
Transport fuel
2003/30/EC
Immobilized cell
description Using a growth medium based on cane blackstrap molasses, we compared ethanol production by two strains of Saccharomyces cerevisiae that were immobilized in polyurethane foam cubes in a fluidised-bed fermenter. One strain (NCYC 1119) was adhesive and extremely flocculent, whilst the other strain was not adhesive and only weakly flocculent. The strong flocs of NCYC 1119 caused blockage of the bed, so that stable operation could not be achieved beyond 15 days. Nevertheless, it was able to produce 40 g L-1 ethanol at a rate up to 16 g L-1 h-1 (D = 0.4 h-1), although this production period was limited to 192 h. In contrast, the non-adhesive strain was only capable of producing 28 g L-1 ethanol at a rate of 11 g L-1 h-1 at the same dilution rate, even though production continued for 576 h. Despite the conversion of sugars to ethanol not being complete during these trials (up to 47 g L-1 was expected), it was clearly demonstrated that the productivity of the adhesive strain was higher than that of the non-adhesive one. However, further work is required to develop this process into a robust, industrial system.
publishDate 2006
dc.date.none.fl_str_mv 2006
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/3792
http://hdl.handle.net/10316/3792
https://doi.org/10.1016/j.enzmictec.2005.12.025
url http://hdl.handle.net/10316/3792
https://doi.org/10.1016/j.enzmictec.2005.12.025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Enzyme and Microbial Technology. 40:1 (2006) 127-131
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dc.format.none.fl_str_mv aplication/PDF
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