Azole-resistant Aspergillus fumigatus harboring the TR34/L98H mutation : first report in Portugal in environmental samples

Detalhes bibliográficos
Autor(a) principal: Gonçalves, Paulo
Data de Publicação: 2020
Outros Autores: Melo, Aryse, Dias, Marta, Almeida, Beatriz, Caetano, Liliana Aranha, Veríssimo, Cristina, Viegas, Carla, Sabino, Raquel Filipa Pinheiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/45688
Resumo: Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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spelling Azole-resistant Aspergillus fumigatus harboring the TR34/L98H mutation : first report in Portugal in environmental samplesAspergillus fumigatusAzolesAntifungal resistanceCyp51A geneEnvironmentOccupational exposureCopyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).The frequency in detection of azole-resistant Aspergillus fumigatus isolates has increased since 2010. In Portugal, the section Fumigati is one of the most frequent, and resistant strains to have been found in clinical and environmental contexts. Although several cryptic species within the Fumigati section show intrinsic resistance to azoles, one factor driving (acquired) resistance is selective pressure deriving from the extensive use of azoles. This is particularly problematic in occupational environments where high fungal loads are expected, and where there is an increased risk of human exposure and infection, with impact on treatment success and disease outcome. The mechanisms of resistance are diverse, but mainly associated with mutations in the cyp51A gene. Despite TR34/L98H being the most frequent mutation described, it has only been detected in clinical specimens in Portugal. Methods: We analyzed 99 A. fumigatus isolates from indoor environments (healthcare facilities, spas, one dairy and one waste sorting unit) collected from January 2018 to February 2019 in different regions of Portugal. Isolates were screened for resistance to itraconazole, voriconazole and posaconazole by culture, and resistance was confirmed by broth microdilution. Sequencing of the cyp51A gene and its promoter was performed to detect mutations associated with resistance. Results: Overall, 8.1% of isolates were able to grow in the presence of at least one azole, and 3% (isolated from the air in a dairy and from filtering respiratory protective devices in a waste sorting industry) were pan-azole-resistant, bearing the TR34/L98H mutation. Conclusion: For the first time in Portugal, we report environmental isolates bearing the TR34/L98H mutation, isolated from occupational environments. Environmental surveillance of the emergence of azole-resistant A. fumigatus sensu stricto strains is needed, to ensure proper and timely implementation of control policies that may have a positive impact on public and occupational health.H&TRC authors gratefully acknowledge the FCT/MCTES national supportthrough the UIDB/05608/2020 and UIDP/05608/2020MDPIRepositório da Universidade de LisboaGonçalves, PauloMelo, AryseDias, MartaAlmeida, BeatrizCaetano, Liliana AranhaVeríssimo, CristinaViegas, CarlaSabino, Raquel Filipa Pinheiro2021-01-07T12:27:02Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/45688engMicroorganisms 2021, 9(1), 5710.3390/microorganisms90100572076-2607info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-14T15:33:11ZPortal AgregadorONG
dc.title.none.fl_str_mv Azole-resistant Aspergillus fumigatus harboring the TR34/L98H mutation : first report in Portugal in environmental samples
title Azole-resistant Aspergillus fumigatus harboring the TR34/L98H mutation : first report in Portugal in environmental samples
spellingShingle Azole-resistant Aspergillus fumigatus harboring the TR34/L98H mutation : first report in Portugal in environmental samples
Gonçalves, Paulo
Aspergillus fumigatus
Azoles
Antifungal resistance
Cyp51A gene
Environment
Occupational exposure
title_short Azole-resistant Aspergillus fumigatus harboring the TR34/L98H mutation : first report in Portugal in environmental samples
title_full Azole-resistant Aspergillus fumigatus harboring the TR34/L98H mutation : first report in Portugal in environmental samples
title_fullStr Azole-resistant Aspergillus fumigatus harboring the TR34/L98H mutation : first report in Portugal in environmental samples
title_full_unstemmed Azole-resistant Aspergillus fumigatus harboring the TR34/L98H mutation : first report in Portugal in environmental samples
title_sort Azole-resistant Aspergillus fumigatus harboring the TR34/L98H mutation : first report in Portugal in environmental samples
author Gonçalves, Paulo
author_facet Gonçalves, Paulo
Melo, Aryse
Dias, Marta
Almeida, Beatriz
Caetano, Liliana Aranha
Veríssimo, Cristina
Viegas, Carla
Sabino, Raquel Filipa Pinheiro
author_role author
author2 Melo, Aryse
Dias, Marta
Almeida, Beatriz
Caetano, Liliana Aranha
Veríssimo, Cristina
Viegas, Carla
Sabino, Raquel Filipa Pinheiro
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Gonçalves, Paulo
Melo, Aryse
Dias, Marta
Almeida, Beatriz
Caetano, Liliana Aranha
Veríssimo, Cristina
Viegas, Carla
Sabino, Raquel Filipa Pinheiro
dc.subject.por.fl_str_mv Aspergillus fumigatus
Azoles
Antifungal resistance
Cyp51A gene
Environment
Occupational exposure
topic Aspergillus fumigatus
Azoles
Antifungal resistance
Cyp51A gene
Environment
Occupational exposure
description Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
2021-01-07T12:27:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/45688
url http://hdl.handle.net/10451/45688
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microorganisms 2021, 9(1), 57
10.3390/microorganisms9010057
2076-2607
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.mail.fl_str_mv
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