Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution

Detalhes bibliográficos
Autor(a) principal: Jota Baptista, C.
Data de Publicação: 2023
Outros Autores: Seixas, F., Gonzalo-Orden, J. M., Patinha, C., Pato, P., Ferreira da Silva, E., Casero, M., Brazio, E., Brandão, R., Costa, D., Mateus, T. L., Oliveira, P. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.48797/sl.2023.57
Resumo: Background: Heavy metal(loid)s pollution is a One Health concern [1]. Hedgehogs (Erinaceus europaeus) are promising candidates for biomonitoring programs, due to their habits, abundance, distribution and resilience [2]. Objective: This work aims to evaluate heavy metal(loid)s pollution, using E. europaeus as a bioindicator. Methods: Necropsies of 46 hedgehogs from three distinct rescue centres (CERVAS, LxCRAS and RIAS) were performed. Provenance and clinical data (when available) were recorded. Sex and age group were estimated. Liver, kidney and external spines (2-10 grams) were collected and stored under -20ºC. Internal organs were completely freeze-dried for two days at -56ºC (LaboGeneCoolSafe®) and stored frozen until further analysis. Spines were washed in an ultrasound machine (Sonorex RK 106®) and dried overnight in an oven (55ºC). Acid digestion was performed in a digestion plate (DigiPrep-MS®) and metal(loid)s concentrations (As, Cd, Cr, Cu, and Pb) were determined with ICP-MS. Liver and kidney were also collected for histopathology routine examination. Results: High levels of Cu were found in the kidney (24.74±21.05 mg kg-1 dry weight [dw]) and liver (35.66 ± 19.65 mg kg-1dw), with some animals passing 100 mg kg-1 dw, which is a high value for insectivores [3]. Significant correlations have been found between spines and liver and between spines and kidney, for Co (p<0.001, in both organs) and Pb (p=0.020 and p=0.019), suggesting spines as a non-invasive sample to access internal metal(loid) concentrations. Biliary hyperplasia was the most frequent lesion observed (36%)- Animals presenting biliary hyperplasia show higher levels of metal(loid)s, with a significant difference for Cd (p=0.007) and Co (p=0.019). Conclusions: Further research, including different locations and organs, is mandatory to comprehend the real impact of metal(loid)s pollution in different Portuguese locations, under a One Health perspective. 
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spelling Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollutionSelected Oral CommunicationBackground: Heavy metal(loid)s pollution is a One Health concern [1]. Hedgehogs (Erinaceus europaeus) are promising candidates for biomonitoring programs, due to their habits, abundance, distribution and resilience [2]. Objective: This work aims to evaluate heavy metal(loid)s pollution, using E. europaeus as a bioindicator. Methods: Necropsies of 46 hedgehogs from three distinct rescue centres (CERVAS, LxCRAS and RIAS) were performed. Provenance and clinical data (when available) were recorded. Sex and age group were estimated. Liver, kidney and external spines (2-10 grams) were collected and stored under -20ºC. Internal organs were completely freeze-dried for two days at -56ºC (LaboGeneCoolSafe®) and stored frozen until further analysis. Spines were washed in an ultrasound machine (Sonorex RK 106®) and dried overnight in an oven (55ºC). Acid digestion was performed in a digestion plate (DigiPrep-MS®) and metal(loid)s concentrations (As, Cd, Cr, Cu, and Pb) were determined with ICP-MS. Liver and kidney were also collected for histopathology routine examination. Results: High levels of Cu were found in the kidney (24.74±21.05 mg kg-1 dry weight [dw]) and liver (35.66 ± 19.65 mg kg-1dw), with some animals passing 100 mg kg-1 dw, which is a high value for insectivores [3]. Significant correlations have been found between spines and liver and between spines and kidney, for Co (p<0.001, in both organs) and Pb (p=0.020 and p=0.019), suggesting spines as a non-invasive sample to access internal metal(loid) concentrations. Biliary hyperplasia was the most frequent lesion observed (36%)- Animals presenting biliary hyperplasia show higher levels of metal(loid)s, with a significant difference for Cd (p=0.007) and Co (p=0.019). Conclusions: Further research, including different locations and organs, is mandatory to comprehend the real impact of metal(loid)s pollution in different Portuguese locations, under a One Health perspective. IUCS-CESPU Publishing2023-04-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.48797/sl.2023.57https://doi.org/10.48797/sl.2023.57Scientific Letters; Vol. 1 No. Sup 1 (2023)2795-5117reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttps://publicacoes.cespu.pt/index.php/sl/article/view/57https://publicacoes.cespu.pt/index.php/sl/article/view/57/111Copyright (c) 2023 C. Jota Baptista, F. Seixas, J. M. Gonzalo-Orden, C. Patinha, P. Pato, E. Ferreira da Silva, M. Casero, E. Brazio, R. Brandão, D. Costa, T. L. Mateus, P. A. Oliveirainfo:eu-repo/semantics/openAccessJota Baptista, C.Seixas, F.Gonzalo-Orden, J. M.Patinha, C.Pato, P.Ferreira da Silva, E.Casero, M.Brazio, E.Brandão, R.Costa, D.Mateus, T. L.Oliveira, P. A.2023-04-29T08:46:03Zoai:publicacoes.cespu.pt:article/57Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:50:22.346693Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution
title Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution
spellingShingle Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution
Jota Baptista, C.
Selected Oral Communication
title_short Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution
title_full Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution
title_fullStr Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution
title_full_unstemmed Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution
title_sort Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution
author Jota Baptista, C.
author_facet Jota Baptista, C.
Seixas, F.
Gonzalo-Orden, J. M.
Patinha, C.
Pato, P.
Ferreira da Silva, E.
Casero, M.
Brazio, E.
Brandão, R.
Costa, D.
Mateus, T. L.
Oliveira, P. A.
author_role author
author2 Seixas, F.
Gonzalo-Orden, J. M.
Patinha, C.
Pato, P.
Ferreira da Silva, E.
Casero, M.
Brazio, E.
Brandão, R.
Costa, D.
Mateus, T. L.
Oliveira, P. A.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Jota Baptista, C.
Seixas, F.
Gonzalo-Orden, J. M.
Patinha, C.
Pato, P.
Ferreira da Silva, E.
Casero, M.
Brazio, E.
Brandão, R.
Costa, D.
Mateus, T. L.
Oliveira, P. A.
dc.subject.por.fl_str_mv Selected Oral Communication
topic Selected Oral Communication
description Background: Heavy metal(loid)s pollution is a One Health concern [1]. Hedgehogs (Erinaceus europaeus) are promising candidates for biomonitoring programs, due to their habits, abundance, distribution and resilience [2]. Objective: This work aims to evaluate heavy metal(loid)s pollution, using E. europaeus as a bioindicator. Methods: Necropsies of 46 hedgehogs from three distinct rescue centres (CERVAS, LxCRAS and RIAS) were performed. Provenance and clinical data (when available) were recorded. Sex and age group were estimated. Liver, kidney and external spines (2-10 grams) were collected and stored under -20ºC. Internal organs were completely freeze-dried for two days at -56ºC (LaboGeneCoolSafe®) and stored frozen until further analysis. Spines were washed in an ultrasound machine (Sonorex RK 106®) and dried overnight in an oven (55ºC). Acid digestion was performed in a digestion plate (DigiPrep-MS®) and metal(loid)s concentrations (As, Cd, Cr, Cu, and Pb) were determined with ICP-MS. Liver and kidney were also collected for histopathology routine examination. Results: High levels of Cu were found in the kidney (24.74±21.05 mg kg-1 dry weight [dw]) and liver (35.66 ± 19.65 mg kg-1dw), with some animals passing 100 mg kg-1 dw, which is a high value for insectivores [3]. Significant correlations have been found between spines and liver and between spines and kidney, for Co (p<0.001, in both organs) and Pb (p=0.020 and p=0.019), suggesting spines as a non-invasive sample to access internal metal(loid) concentrations. Biliary hyperplasia was the most frequent lesion observed (36%)- Animals presenting biliary hyperplasia show higher levels of metal(loid)s, with a significant difference for Cd (p=0.007) and Co (p=0.019). Conclusions: Further research, including different locations and organs, is mandatory to comprehend the real impact of metal(loid)s pollution in different Portuguese locations, under a One Health perspective. 
publishDate 2023
dc.date.none.fl_str_mv 2023-04-21
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.48797/sl.2023.57
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url https://doi.org/10.48797/sl.2023.57
dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv https://publicacoes.cespu.pt/index.php/sl/article/view/57
https://publicacoes.cespu.pt/index.php/sl/article/view/57/111
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dc.publisher.none.fl_str_mv IUCS-CESPU Publishing
publisher.none.fl_str_mv IUCS-CESPU Publishing
dc.source.none.fl_str_mv Scientific Letters; Vol. 1 No. Sup 1 (2023)
2795-5117
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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