Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.48797/sl.2023.57 |
Resumo: | Background: Heavy metal(loid)s pollution is a One Health concern [1]. Hedgehogs (Erinaceus europaeus) are promising candidates for biomonitoring programs, due to their habits, abundance, distribution and resilience [2]. Objective: This work aims to evaluate heavy metal(loid)s pollution, using E. europaeus as a bioindicator. Methods: Necropsies of 46 hedgehogs from three distinct rescue centres (CERVAS, LxCRAS and RIAS) were performed. Provenance and clinical data (when available) were recorded. Sex and age group were estimated. Liver, kidney and external spines (2-10 grams) were collected and stored under -20ºC. Internal organs were completely freeze-dried for two days at -56ºC (LaboGeneCoolSafe®) and stored frozen until further analysis. Spines were washed in an ultrasound machine (Sonorex RK 106®) and dried overnight in an oven (55ºC). Acid digestion was performed in a digestion plate (DigiPrep-MS®) and metal(loid)s concentrations (As, Cd, Cr, Cu, and Pb) were determined with ICP-MS. Liver and kidney were also collected for histopathology routine examination. Results: High levels of Cu were found in the kidney (24.74±21.05 mg kg-1 dry weight [dw]) and liver (35.66 ± 19.65 mg kg-1dw), with some animals passing 100 mg kg-1 dw, which is a high value for insectivores [3]. Significant correlations have been found between spines and liver and between spines and kidney, for Co (p<0.001, in both organs) and Pb (p=0.020 and p=0.019), suggesting spines as a non-invasive sample to access internal metal(loid) concentrations. Biliary hyperplasia was the most frequent lesion observed (36%)- Animals presenting biliary hyperplasia show higher levels of metal(loid)s, with a significant difference for Cd (p=0.007) and Co (p=0.019). Conclusions: Further research, including different locations and organs, is mandatory to comprehend the real impact of metal(loid)s pollution in different Portuguese locations, under a One Health perspective. |
id |
RCAP_43b4d9ebd0cd5f62a8331657c9099836 |
---|---|
oai_identifier_str |
oai:publicacoes.cespu.pt:article/57 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollutionSelected Oral CommunicationBackground: Heavy metal(loid)s pollution is a One Health concern [1]. Hedgehogs (Erinaceus europaeus) are promising candidates for biomonitoring programs, due to their habits, abundance, distribution and resilience [2]. Objective: This work aims to evaluate heavy metal(loid)s pollution, using E. europaeus as a bioindicator. Methods: Necropsies of 46 hedgehogs from three distinct rescue centres (CERVAS, LxCRAS and RIAS) were performed. Provenance and clinical data (when available) were recorded. Sex and age group were estimated. Liver, kidney and external spines (2-10 grams) were collected and stored under -20ºC. Internal organs were completely freeze-dried for two days at -56ºC (LaboGeneCoolSafe®) and stored frozen until further analysis. Spines were washed in an ultrasound machine (Sonorex RK 106®) and dried overnight in an oven (55ºC). Acid digestion was performed in a digestion plate (DigiPrep-MS®) and metal(loid)s concentrations (As, Cd, Cr, Cu, and Pb) were determined with ICP-MS. Liver and kidney were also collected for histopathology routine examination. Results: High levels of Cu were found in the kidney (24.74±21.05 mg kg-1 dry weight [dw]) and liver (35.66 ± 19.65 mg kg-1dw), with some animals passing 100 mg kg-1 dw, which is a high value for insectivores [3]. Significant correlations have been found between spines and liver and between spines and kidney, for Co (p<0.001, in both organs) and Pb (p=0.020 and p=0.019), suggesting spines as a non-invasive sample to access internal metal(loid) concentrations. Biliary hyperplasia was the most frequent lesion observed (36%)- Animals presenting biliary hyperplasia show higher levels of metal(loid)s, with a significant difference for Cd (p=0.007) and Co (p=0.019). Conclusions: Further research, including different locations and organs, is mandatory to comprehend the real impact of metal(loid)s pollution in different Portuguese locations, under a One Health perspective. IUCS-CESPU Publishing2023-04-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.48797/sl.2023.57https://doi.org/10.48797/sl.2023.57Scientific Letters; Vol. 1 No. Sup 1 (2023)2795-5117reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttps://publicacoes.cespu.pt/index.php/sl/article/view/57https://publicacoes.cespu.pt/index.php/sl/article/view/57/111Copyright (c) 2023 C. Jota Baptista, F. Seixas, J. M. Gonzalo-Orden, C. Patinha, P. Pato, E. Ferreira da Silva, M. Casero, E. Brazio, R. Brandão, D. Costa, T. L. Mateus, P. A. Oliveirainfo:eu-repo/semantics/openAccessJota Baptista, C.Seixas, F.Gonzalo-Orden, J. M.Patinha, C.Pato, P.Ferreira da Silva, E.Casero, M.Brazio, E.Brandão, R.Costa, D.Mateus, T. L.Oliveira, P. A.2023-04-29T08:46:03Zoai:publicacoes.cespu.pt:article/57Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:50:22.346693Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution |
title |
Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution |
spellingShingle |
Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution Jota Baptista, C. Selected Oral Communication |
title_short |
Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution |
title_full |
Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution |
title_fullStr |
Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution |
title_full_unstemmed |
Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution |
title_sort |
Hedgehogs (Erinaceus europaeus) as bioindicators of heavy metal(loid) pollution |
author |
Jota Baptista, C. |
author_facet |
Jota Baptista, C. Seixas, F. Gonzalo-Orden, J. M. Patinha, C. Pato, P. Ferreira da Silva, E. Casero, M. Brazio, E. Brandão, R. Costa, D. Mateus, T. L. Oliveira, P. A. |
author_role |
author |
author2 |
Seixas, F. Gonzalo-Orden, J. M. Patinha, C. Pato, P. Ferreira da Silva, E. Casero, M. Brazio, E. Brandão, R. Costa, D. Mateus, T. L. Oliveira, P. A. |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Jota Baptista, C. Seixas, F. Gonzalo-Orden, J. M. Patinha, C. Pato, P. Ferreira da Silva, E. Casero, M. Brazio, E. Brandão, R. Costa, D. Mateus, T. L. Oliveira, P. A. |
dc.subject.por.fl_str_mv |
Selected Oral Communication |
topic |
Selected Oral Communication |
description |
Background: Heavy metal(loid)s pollution is a One Health concern [1]. Hedgehogs (Erinaceus europaeus) are promising candidates for biomonitoring programs, due to their habits, abundance, distribution and resilience [2]. Objective: This work aims to evaluate heavy metal(loid)s pollution, using E. europaeus as a bioindicator. Methods: Necropsies of 46 hedgehogs from three distinct rescue centres (CERVAS, LxCRAS and RIAS) were performed. Provenance and clinical data (when available) were recorded. Sex and age group were estimated. Liver, kidney and external spines (2-10 grams) were collected and stored under -20ºC. Internal organs were completely freeze-dried for two days at -56ºC (LaboGeneCoolSafe®) and stored frozen until further analysis. Spines were washed in an ultrasound machine (Sonorex RK 106®) and dried overnight in an oven (55ºC). Acid digestion was performed in a digestion plate (DigiPrep-MS®) and metal(loid)s concentrations (As, Cd, Cr, Cu, and Pb) were determined with ICP-MS. Liver and kidney were also collected for histopathology routine examination. Results: High levels of Cu were found in the kidney (24.74±21.05 mg kg-1 dry weight [dw]) and liver (35.66 ± 19.65 mg kg-1dw), with some animals passing 100 mg kg-1 dw, which is a high value for insectivores [3]. Significant correlations have been found between spines and liver and between spines and kidney, for Co (p<0.001, in both organs) and Pb (p=0.020 and p=0.019), suggesting spines as a non-invasive sample to access internal metal(loid) concentrations. Biliary hyperplasia was the most frequent lesion observed (36%)- Animals presenting biliary hyperplasia show higher levels of metal(loid)s, with a significant difference for Cd (p=0.007) and Co (p=0.019). Conclusions: Further research, including different locations and organs, is mandatory to comprehend the real impact of metal(loid)s pollution in different Portuguese locations, under a One Health perspective. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-04-21 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.48797/sl.2023.57 https://doi.org/10.48797/sl.2023.57 |
url |
https://doi.org/10.48797/sl.2023.57 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://publicacoes.cespu.pt/index.php/sl/article/view/57 https://publicacoes.cespu.pt/index.php/sl/article/view/57/111 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
IUCS-CESPU Publishing |
publisher.none.fl_str_mv |
IUCS-CESPU Publishing |
dc.source.none.fl_str_mv |
Scientific Letters; Vol. 1 No. Sup 1 (2023) 2795-5117 reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131583714689024 |