Dermal papilla cells and melanocytes response to physiological oxygen levels depends on their interactions

Detalhes bibliográficos
Autor(a) principal: Abreu, C. M.
Data de Publicação: 2021
Outros Autores: Reis, R. L., Marques, A. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/75130
Resumo: Background: Human dermal papilla (DP) cells and melanocytes (hMel) are central players in hair growth and pigmentation, respectively. In hair follicles (HFs), oxygen (O2) levels average 5%, being coupled with the production of reactive oxygen species (ROS), necessary to promote hair growth.Materials and Methods: DP cell and hMel proliferation and phenotype were stud-ied under physiological (5%O2, physoxia) or atmospheric (21%O2, normoxia) oxygen levels. hMel-DP cells interactions were studied in indirect co-culture or by directly co-culturing hMel with DP spheroids, to test whether their interaction affected the response to physoxia.Results: Physoxia decreased DP cell senescence and improved their secretome and phenotype, as well as hMel proliferation, migration, and tyrosinase activity. In indi-rect co- cultures, physoxia affected DP cellsâ alkaline phosphatase (ALP) activity but their signalling did not influence hMel proliferation or tyrosinase activity. Additionally, ROS production was higher than in monocultures but a direct correlation between ROS generation and ALP activity in DP cells was not observed. In the 3D aggregates, where hMel are organized around the DP, both hMel tyrosinase and DP cells ALP ac-tivities, their main functional indicators, plus ROS production were higher in physoxia than normoxia.Conclusions: Overall, we showed that the response to physoxia differs according to hMel-DP cells interactions and that the microenvironment recreated when in direct contact favours their functions, which can be relevant for hair regeneration purposes.
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spelling Dermal papilla cells and melanocytes response to physiological oxygen levels depends on their interactionsdermal papillahair folliclemelanocytescellular interactionsdermal papilla cellsphysoxiaEngenharia e Tecnologia::Outras Engenharias e TecnologiasScience & TechnologyBackground: Human dermal papilla (DP) cells and melanocytes (hMel) are central players in hair growth and pigmentation, respectively. In hair follicles (HFs), oxygen (O2) levels average 5%, being coupled with the production of reactive oxygen species (ROS), necessary to promote hair growth.Materials and Methods: DP cell and hMel proliferation and phenotype were stud-ied under physiological (5%O2, physoxia) or atmospheric (21%O2, normoxia) oxygen levels. hMel-DP cells interactions were studied in indirect co-culture or by directly co-culturing hMel with DP spheroids, to test whether their interaction affected the response to physoxia.Results: Physoxia decreased DP cell senescence and improved their secretome and phenotype, as well as hMel proliferation, migration, and tyrosinase activity. In indi-rect co- cultures, physoxia affected DP cellsâ alkaline phosphatase (ALP) activity but their signalling did not influence hMel proliferation or tyrosinase activity. Additionally, ROS production was higher than in monocultures but a direct correlation between ROS generation and ALP activity in DP cells was not observed. In the 3D aggregates, where hMel are organized around the DP, both hMel tyrosinase and DP cells ALP ac-tivities, their main functional indicators, plus ROS production were higher in physoxia than normoxia.Conclusions: Overall, we showed that the response to physoxia differs according to hMel-DP cells interactions and that the microenvironment recreated when in direct contact favours their functions, which can be relevant for hair regeneration purposes.We thank Dr Luca Gasperini for his technical assistance in the image analysis using the CellProfiler™ software. The authors also thank the financial support given by the European Research Council through the consolidator grant “ECM_INK” (ERC-2016-COG-726061) and by FCT/MCTES (Fundação para a Ciência e a Tecnologia/ Ministério da Ciência, Tecnologia, e Ensino Superior) through the PD/59/2013, PD/BD/113800/2015 (C. Abreu) and IF/00945/2014 (A. P. Marques) grants.John Wiley & Sons LtdUniversidade do MinhoAbreu, C. M.Reis, R. L.Marques, A. P.2021-022021-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/75130engAbreu C. M., Reis R. L., Marques A. P. Dermal papilla cells and melanocytes response to physiological oxygen levels depends on their interactions, Cell Proliferation, pp. e13013, doi:10.1111/cpr.13013, 20211365-218410.1111/cpr.1301334101928e13013https://onlinelibrary.wiley.com/doi/epdf/10.1111/cpr.13013info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:54:41Zoai:repositorium.sdum.uminho.pt:1822/75130Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:44:09.832947Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Dermal papilla cells and melanocytes response to physiological oxygen levels depends on their interactions
title Dermal papilla cells and melanocytes response to physiological oxygen levels depends on their interactions
spellingShingle Dermal papilla cells and melanocytes response to physiological oxygen levels depends on their interactions
Abreu, C. M.
dermal papilla
hair follicle
melanocytes
cellular interactions
dermal papilla cells
physoxia
Engenharia e Tecnologia::Outras Engenharias e Tecnologias
Science & Technology
title_short Dermal papilla cells and melanocytes response to physiological oxygen levels depends on their interactions
title_full Dermal papilla cells and melanocytes response to physiological oxygen levels depends on their interactions
title_fullStr Dermal papilla cells and melanocytes response to physiological oxygen levels depends on their interactions
title_full_unstemmed Dermal papilla cells and melanocytes response to physiological oxygen levels depends on their interactions
title_sort Dermal papilla cells and melanocytes response to physiological oxygen levels depends on their interactions
author Abreu, C. M.
author_facet Abreu, C. M.
Reis, R. L.
Marques, A. P.
author_role author
author2 Reis, R. L.
Marques, A. P.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Abreu, C. M.
Reis, R. L.
Marques, A. P.
dc.subject.por.fl_str_mv dermal papilla
hair follicle
melanocytes
cellular interactions
dermal papilla cells
physoxia
Engenharia e Tecnologia::Outras Engenharias e Tecnologias
Science & Technology
topic dermal papilla
hair follicle
melanocytes
cellular interactions
dermal papilla cells
physoxia
Engenharia e Tecnologia::Outras Engenharias e Tecnologias
Science & Technology
description Background: Human dermal papilla (DP) cells and melanocytes (hMel) are central players in hair growth and pigmentation, respectively. In hair follicles (HFs), oxygen (O2) levels average 5%, being coupled with the production of reactive oxygen species (ROS), necessary to promote hair growth.Materials and Methods: DP cell and hMel proliferation and phenotype were stud-ied under physiological (5%O2, physoxia) or atmospheric (21%O2, normoxia) oxygen levels. hMel-DP cells interactions were studied in indirect co-culture or by directly co-culturing hMel with DP spheroids, to test whether their interaction affected the response to physoxia.Results: Physoxia decreased DP cell senescence and improved their secretome and phenotype, as well as hMel proliferation, migration, and tyrosinase activity. In indi-rect co- cultures, physoxia affected DP cellsâ alkaline phosphatase (ALP) activity but their signalling did not influence hMel proliferation or tyrosinase activity. Additionally, ROS production was higher than in monocultures but a direct correlation between ROS generation and ALP activity in DP cells was not observed. In the 3D aggregates, where hMel are organized around the DP, both hMel tyrosinase and DP cells ALP ac-tivities, their main functional indicators, plus ROS production were higher in physoxia than normoxia.Conclusions: Overall, we showed that the response to physoxia differs according to hMel-DP cells interactions and that the microenvironment recreated when in direct contact favours their functions, which can be relevant for hair regeneration purposes.
publishDate 2021
dc.date.none.fl_str_mv 2021-02
2021-02-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/75130
url http://hdl.handle.net/1822/75130
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Abreu C. M., Reis R. L., Marques A. P. Dermal papilla cells and melanocytes response to physiological oxygen levels depends on their interactions, Cell Proliferation, pp. e13013, doi:10.1111/cpr.13013, 2021
1365-2184
10.1111/cpr.13013
34101928
e13013
https://onlinelibrary.wiley.com/doi/epdf/10.1111/cpr.13013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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